Project/Area Number |
01570125
|
Research Category |
Grant-in-Aid for General Scientific Research (C)
|
Allocation Type | Single-year Grants |
Research Field |
General medical chemistry
|
Research Institution | University of Tokyo |
Principal Investigator |
WATANABE Tsuyoshi Assistant Professor Dept. of Int. Med., University of Tokyo, 医学部(病)・第一内科, 助手 (80158641)
|
Co-Investigator(Kenkyū-buntansha) |
TERAMOTO Tamio Assistant Professor, Dept. of Int. Med., University of Tokyo, 医学部(病)第一内科, 助手 (20133077)
SHIMIZU Takao Associate Professor, Dept. of Nutrition and Physiological Chemistry, University, 医学部栄養学, 助教授 (80127092)
|
Project Period (FY) |
1989 – 1990
|
Project Status |
Completed (Fiscal Year 1990)
|
Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
Keywords | Leukoriene / Lung / Receptor / Solubilization / Pertussis Toxin / Molecular Cloning / Oocytes / GTP-binding protein / ロイコトリエンD_4 / モルモット肺 |
Research Abstract |
The objective of this project is the molecular cloning or purification of leukotriene (LT) D_4 or B_4 receptor for the understanding of signal transduction mechanism of leukotiene at the molecular level. We solubilized LTD_4 receptor in an active form from guinea pig lung and LTB_4 receptor from porcine spleen using a detergent (CHAPS) for the first tine. Using this solubilized preparation, we could partially purify the receptor and characterize the receptor protein and its associated GTP-binding protein (Ref. 1,2). We constructed cDNA library from guinea pig lung mRNA. Using zenopus oocyte expression system, we tried to get a clone encoding LTD_4 receptor and also Platelet-Activating Factor(PAF) receptor from this library. We are still trying for LTD_4 receptor, but have gotten a clone encoding PAF receptor. We clarified the structure of the receptor protein and also succeeded to express the receptor in a mammalian (COS) cells.
|