| Project/Area Number |
01570142
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Juntendo University School of Medicine |
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Principal Investigator |
KITA Kiyoshi Juntendo University, Department of Parasitology, Assistant Professor, 医学部, 講師 (90134444)
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| Co-Investigator(Kenkyū-buntansha) |
FURUSHIMA Rieko Juntendo University, Department of Parasitology Research Assistant, 医学部, 助手 (50146776)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | complex II / cytochrome b / mitochondria / cloning / cDNA sequence / サブユニットの解離 |
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| Research Abstract |
Complex II (succinate-ubiquinone oxidoreductase) is an important enzyme complex of both the TCA cycle and of the aerobic respiratory chain of mitochondria in eukaryotic cell and prokaryotic organisms. In this study, we have characterized the cytochrome b in complex II as described below.
1) To dissociate the subunits of complex II, ionic detergent "sarkosyl" was most useful.
2) In vivo reconstitution system of complex II from sdh genes separated in multiple plasmids in the Escherichia coli Cell has been established.
3) cDNA library of Ascarismuscle has been constructed and positive clones for cytochorme b subunit of compleX II have been screened immunologically.
4) Amino acid sequence of iron-sulfur subunit in human liver was deduced from cDNA which was isolated by immunoscreening.
5) Oxidation-reduction potentials of cytochromes in muscle mitochondria of Ascaris and purified complex II were determined and compared with those in mammalian mitochondria. Oxidation-reduction potential of cytochrome b_<558> of Ascaris complex II was higher than that of bovine heart complex II.
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