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Stimulation Mechanism of Calcifiction by Prostagland in D_2 in Human Osteoblasts

Research Project

Project/Area Number 01570146
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

KOSHIHARA Yasuko  Dept. of Biosignal Research, Senior Research, 生体情報部門, 主任研究員 (20073025)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsProstaglandin / Prostaglandin D_2 / Calcification / Osteoblast / Human
Research Abstract

It is important to study on the age-related mechanism to cause osteoporosis and on the medical treatment for it, as well as to study on senile dementia occurring among increasingly aged persons.
In recent years, several investigators have demonstrated that bone metabolism is regulated with various kinds of hormones and autacoids, which have multitudious effects in a small amount. We have been studying a intriguing regulation mechanism of bone formation by prostaglandins. Meanwhile we recently found that prostaglandin D_2 stimulates mineralization in cultured human osteoblasts, and then have the interesting results on stimulation mechanism, demonstrating for these two years as follows.
1) Prostaglandin D_2 in culture medium is found to be converted to ^<12>*-prostaglandin J_2 with serum albumin. Finally the active form has identified as ^<12>*-prostaglandin J_2. Cyclopentenon ring of the compound is essential to give the effect, but C-15 hydroxy is not, as the experimental results that various kinds of derivatives have been used to clarify the effect.
2) ^<12>*-prostaglandin J_2 actually increases calcium ion influx into cells, participating in the mechanism on mineralization. The treatment with ^<12>*-prostagladin J_2 for 20 days increases miniralization by 2-3 folds.
3) The mineralizaion is based on bone collagen. It depends on an enhanced expression of collagen genes, demonstrating by the northern blot analysis for procollagen alpha^1 (I) mRNA.
4) Osteocalsin, a non-collagenous protein, physiologically active in bone metabolism, is not affected on its release from cells, when ^<12>*-prostaglandin J_2 is added. However, Its accumulaton in cells is increased as well as an active form of vitamin D_3.
5) Presumably, ^<12>*-prostaglandin J_2 directly penetrates into cells without specific receptor(s), since it does not affect on intracellular CAMP level.
Further functions of prostaglandins involving the age-related metabolism in bone should be examined by causal analyses.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Yasuko Koshihara,et al.: "Prostaglandin D_2 stimulates calcification of human osteoblastic cells" Biochem.Biophys.Res.Commun.159. 1206-1212 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara,et al.: "Establishment of human osteoblastic cells derived from periosteum in culture" In Vitro Cell.Develop.Biol.25. 37-43 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara,et al.: "Prostaglandin D_2 stimulates calcification by human osteoblastic cells" Adv.Prost.Thromb.Leuk.Res.21. 847-850 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara,et al.: "Mineralization ability of cultured human osteoblastlike periosteal cells does not decline with aging" J.Gerontology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoko Tasaki,et al.: "Prostaglandin D_2 metabolite stimulates collagen synthesis by human osteoblasts during calcification" Prostaglandins. 41. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 腰原 康子: "培養ヒト骨芽細胞の形態と機能" 代謝. 28. i-ii (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 腰原 康子(分担): "骨の老化" (財)東京都老人総合研究所, 182 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 腰原 康子(分担): "骨粗鬆症研究の進歩" トプコ出版, 137 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara, Mieko Kawamura: "Prostaglandin D_2 stimulates calcification of human osteoblastic cells." Biochem. Biophys. Res. Commun. 159. 1206-1212 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara, Mieko Kawamura, Sachiko Endo, Chiharu Tsutsumi, Hiroaki Kodama, Hiromi Oda, Shohzo Higaki: "Establishment of human osteoblastic cells derived from periosteum in culture." In Vitro Cell. Develop. Biol.25. 37-43 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara, Toshikazu Amano, Riko Takamori: "Prpostaglandin D_2 stimulates calcification by human osteoblastic cells." Adv. Prost. Thromb. Leuk. Res.21. 847-850 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoko Tasaki, Riko Takamori, Yasuko Koshihara: "Prostaglandin D_2 metabolite stimulates collagen synthesis by osteoblasts during calcification." Prostaglandins. 41. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara, Michiko Hirano, Mieko Kawamura Hiromi Oda, Shohzo Higaki: "Mineralization ability of cultured human osteoblastlike periosteal cells does not decline with aging." J. Gerontology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara, Riko Takamori, Kohji Nomura, Satoshi Sugiura, Seizi Kurozumi: "Enhancement of in vitro mineralization in human osteoblasts by a novel prostaglandin A_1 derivative TEI-3313 :" J. Pharmac. Exp. Ther.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasuko Koshihara et al.: "Prostagladin D_2 stimulates calcification by human osteoblastic cells" Adv.Prost.Thromb.Leuk.Res.21. 847-850 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuko Koshihara et al.: "Mineralization ability of cultured human osteoblastーlike periosteal cells does not decline with aging" J.Gerontology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoko Tasaki et al.: "Prostaglandin D_2 metabolite stimulates collagen synthesis by human osteoblasts during calcification" Prostaglandins. 41. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuko Koshihara et al.: "Enhancement of in vitro mineralization in human osteoblasts by a novel prostaglandin A_1 derivative TEI 3313" J.Pharmac.Exp.Ther.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 黒木 健文 他: "ヒト骨芽細胞の石灰化に対するサイトカインの抑制作用" 炎症. 10. 403-404 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 腰原 康子: "培養ヒト骨芽細胞の形態と機能" 代謝. 28. i-ii (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 腰原 康子(分担): "骨粗鬆症研究の進鉄" トプコ出版, 137 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 腰原 康子(分担): "骨の老化" (財)東京都老人総合研究所, 182 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yasuko Koshihara: "Prostaglandin D_2 Stimulates calcification of human osteoblastic cells" Biochem,Biophys,Res,Commun.159. 1206-1212 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yasuko Koshihara: "Hydrocortisone enhances calcification of human osteolasts" J.Cell Physiol.

    • Related Report
      1989 Annual Research Report
  • [Publications] 腰原康子: "ヒト培養骨芽細胞の老化に伴う機能の変化II 特に石灰化について" 基礎老化研究. 13. 169-170 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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