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Neural Regulation of Glucose Transport System in Peripheral Tissues.

Research Project

Project/Area Number 01570161
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionEhime University

Principal Investigator

SHIMAZU Takashi (1990)  Ehime School of University Medicine, Professor, 医学部, 教授 (30090400)

高橋 章 (1989)  愛媛大学, 医学部, 助手 (40171467)

Co-Investigator(Kenkyū-buntansha) IWAI Masaru  Ehime School of University Medicine, Assistant Professor, 医学部, 助手 (00184854)
OKUMURA Nobuaki  Ehime School of University Medicine, Assistant Professor, 医学部, 助手 (20224173)
嶋津 孝  愛媛大学, 医学部, 教授 (30090400)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsGlucose transporter / Tissue glucose uptake / Cytochalasin B binding / Ventromedial hypothalamus / Sympathetic nerve / Brown adipose tissue / Heart muscle / Sarcolemmal membrane / 糖輸送活性 / 骨格筋 / 視床下部腹内側核(VMH) / 2-デオキシグルコ-ス / 糖の取り込み / 糖輸送体動態 / 心筋,骨格筋 / 褐色脂肪組織(BAT)
Research Abstract

To clarify the role of the ventromedial hypothalamus (VMH) -sympathetic nervous system in insulin-independent glucose transport in peripheral tissues, tissue glucose uptake was assessed in vivo by the 2-[^3H]deoxyglucose method during electrical and chemical stimulation of the VMH in anesthetized rats. VMH stimulation increased the rate constant of glucose uptake in brown adipose tissue (BAT) (about 10-fold), heart (3-fold) and skeletal muscles (1.5-fold), but not in white adipose tissue, diaphragm and brain. The effect of VMH stimulation on glucose uptake in BAT was abolished by local sympathetic denervation, indicating that the increase in glucose uptake is mediated by the sympathetic nerves. Electrical stimulation of the lateral hypothalamus, on the other hand, had no appreciable effects on 2-[^3H] deoxyglucose uptake in any tissues.
The mechanism of hypothalamic effect on glucose uptake was analyzed by measuring D-glucose transport in vitro, using sarcolemmal membrane vesicles isolated from heart muscle. Non-specific L-glucose transport was subtracted from D-glucose value to obtain specific D-glucose transport. It was found that VMH stimulation caused significant increase in D-glucose transport in heart plasma membrane, as observed after insulin treatment.
Changes in glucose transporters after VMH stimulation were also examined by the [^3H] cytochalasin B binding method using heart sarcolemmal membrane. Scatchard analysis of cytochalasin B binding indicated that VMH stimulation did not alter both the number and affinity (dissociation constant) of glucose transporters in the heart sarcolemmal membrane, whereas insulin administration increased the number of transporters in the membrane. These results suggest that the mechanism by which VMH-sympathetic nervous system enhances glucose uptake in certain tissues is different from that of insulin, probably deriving from an activation of glucose transporter molecule in the plasma membrane.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Saito,M.,Minokoshi,Y.and Shimazu,T.: "Accelerated norepinephirine turnover in peripheral tissues after ventromedial hypothalamic stimulation in rats." Brain Res.481. 298-303 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe,Y.,Takahashi,A.and Shimazu,T.: "Neural control of biosythesis and secretion of serum transferrin in perfused rat liver." Biochem. J.267. 545-548 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe,M.,Saito,M.and Shimazu,T.: "Neuropeptide Y in the specific hypothalamic nuclei of rats treated neonatally with monosodium glutamate." Brain Res.Bull.24. 289-291 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Minokoshi,Y.,Saito,M.and Shimazu,T.: "Adrenergic blockade paradoxically increases lipogenic response of brown adipose tissue to sympathetic nerve stimulation." Neurosci.Lett.109. 341-346 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe,M.,Saito,M.,Ikeda,H.and Shimazu,T.: "Increased neuropeptide Y content in the arcuateーparaventricular hypothalamic neuronal system in both insulinーdependent and nonーinsulinーdependent diabetic rats." Brain Res.539. 223-227 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimazu,T.,Sudo,M.,Minokoshi,Y.and Takahashi,A.: "Role of the hypothalamus in insulinーindependent glucose uptake in peripheral tissues." Brain Res.Bull.in press. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimazu,T.,Minokoshi,T.,Saito,M. and Sudo,M.: "Progress in Obesity Research 1990" John Libbey&Co.(London), (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito,M.,Shimazu,Y.,Minokoshi,Y.and Shimazu,T.: "Progress in Obesity Research 1990" John Libbey&Co.(London), (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito, M., Minokoshi, Y., and Shimazu, T.: "Accelerated norepinephrine turnover in peripheral tissues after ventromedial hypothalamic stimulation in rats." Brain Res.481. 298-303 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe, Y., Takahashi A., and Shimazu, T.: "Neural control of biosynthesis and secretion of serum transferrin in perfused rat liver." Biochem. J.267. 545-548 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe, M., Saito, M., and Shimazu, T.: "Neuropeptide Y in the specific hypothalamic nuclei of rats treated neonatally with monosodium glutamate." Brain Res. Bull.24. 289-291 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Minokoshi, Y., Saito, M., and Shimazu, T.: "Adrenergic blockade paradoxically increases lipogenic response of brown adipose tissue to sympathetic nerve stimulation." Neurosci. Lett.109. 341-346 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe, M., Saito, M., Ikeda, H., and Shimazu, T.: "Increased neuropeptide Y content in the arcuate-paraventricular hypothalamic neuronal system in both insulin-dependent and non-insulin-dependent diabetic rats." Brain Res.539. 223-227 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimazu, T., Sudo, M., Minokochi Y., and Takahashi, A.: "Role of the hypothalamus in insulin-independent glucose uptake in peripheral tissues." Brain Res. Bull.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimazu, T., Minokoshi, Y., Saito, M., and Sudo, M.: "The role of the brain in the control of energy expenditure." Progress in Obesity Research 1990. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Saito, M., Shimazu, Y., Minokoshi, Y., and Shimazu, T.: "Hypothalamic and sympathetic nerve control of brown adipose tissue metabolism and thermogenesis." Progress in Obesity Research 1990. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe,M.,Saito,M.and Shimazu,T.: "Neuropeptide Y in the specific hypothalamic nuclei of rats treated neonatally with monosodium glutamate." Brain Res.Bull.24. 289-291 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Minokoshi,Y.,Saito,M.and Shimazu,T.: "Adrenergic blockade paradoxically increases lipogenic response of brown adipose tissue to sympathetic nerve stimulation." Neurosci.Lett.109. 341-346 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Abe,M.,Saito,M.,Ikeda,H.and Shimazu,T.: "Incrased neuropeptide Y content in the arcuateーparaventricular hypothalamic neuronal system in both insulinーdependent and nonーinsulinーdependent diabetic rats." Brain Res.539. 223-227 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimazu,T.,Sudo,M.,Minokoshi,Y.and Takahashi,A.: "Role of the hypothalamus in insulinーindependent glucose uptake in peripheral tissues." Brain Res.Bull.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高橋 章,嶋津 孝: "筋肉のエネルギ-代謝調節と自律神経系" 代謝. 27. 287-299 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimazu,T.,Minokoshi,Y.,Saito,M.and Sudo,M.: "Progress in Obesity Research 1990" John Libbey & Co.(London), (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Saito,M.,Shimizu,Y.,Minokoshi,Y.and Shimazu,T.: "Progress in Obesity Research 1990" John Libbey & Co.(London), (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Watanabe,Y.,Takahashi,A.and Shimazu,T.: "Neural control of biosynthesis and secretion of serum transferrin in perfused rat liver." Biochem.J.267. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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