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Purification and Characterization of a Rat Brain Aldehyde Dehydrogenase Able to Metabolize gamma-Amino Butyraldehyde to gamma-Aminobutyric Acid

Research Project

Project/Area Number 01570167
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionJikei University School of Medicine

Principal Investigator

OHKAWA Kiyoshi  Jikei University School of Medicine Instructor, 医学部, 講師 (90112812)

Co-Investigator(Kenkyū-buntansha) TAKADA Koji  Jikei University School of Medicine Assistant, 医学部, 助手 (30179452)
ASAKURA Tadashi  Jikei University School of Medicine Assistant, 医学部, 助手 (30138705)
MATSUDA Makoto  Jikei University School of Medicene Professor, 医学部, 教授 (80056506)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsAldehyde dehydrogenase / GABA / 4-aminobutyraldehyde / 4ーアミノブチルアルデヒド / 4-アミノブチルアルデヒド
Research Abstract

An enzyme which catalyses dehydrogenation of gamma-aminobutyraldehyde (ABAL) to gamma-aminobutyric acid (GABA) was purified to homogeneity from rat brain tissues by using DEAE cellulose and affinity chromatography on 5'-AMP-Sepharose and Blue agarose, followed by gel filtration. Such an enzyme was first purified from mammalian brain tissues, and was identified as isoenzyme of aldehyde dehydrogenase. It has an 210 kDa determined by PAEG, and appeared to be composed of subunits of 50 kDa. Substrate specificity of the enzyme toward ABAL was higher than those of aldehyde dehydrogenases from human liver (E2), and was lower than those of ABAL dehydrogenase from human liver (E3). The molecular weight and relative amino acid composition of the enzyme are also similar to those of E1 and E2. Polyclonal and monoclonal antibodies against the purified enzyme were generated. Both antibodies reacted weakly but significantly with a molecule of 50 kDa band of the enzyme by SDS-PAGE, western blot analysis. By immunohistochemical analysis of the rat brains, antibodies showed positive, homogenous staining in the cytoplasms of the nerve cells without any special localization or tissue distribution of the reactive products. The existence of this enzyme in mammalian brain seems to be related to a glutamate decarboxylase-independent pathway for GABA synthesis from putrescine.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Abe,T.: "Purification and characterization of a rat aldehyde dehvdrogenase able to metabolize γーaminobutvraldehvde to γーaminbutvric acid" Biochemical J.269. 25-29 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Asakura,T.: "Effect of gabaculine on metabolism and release of γーaminobutric acid in synaptosomes" Neurochem.Res.15. 295-300 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 阿部 俊夫: "鶏胚脳発生とγーアミノブチルアルデヒド脱水素酵素活性" 医学のあゆみ. 155. in press (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takada,K.: "Substrate specificity of enzymes oxidizing 4ーaminobutyraldenyde to GABA in rat brain" Jikeikai Med.J.36. 317-325 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Asakura,T.: "Distribution of activity converting 4ーaminobutyraldehyde to GABA in subcellular fractions of mouse brain" J.Neurochem.52. 448-452 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe, T., Takada, K., Ohkawa, K., & Matsuda, M.: "Purification and characterization of a rat brain aldehyde dehydrogenase able to metabolize gamma-aminobutyraldehyde to gamma-aminobutyric acid" Biochemical J.269. 25-29 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takada, K, Abe, T., Ohkawa, K., & Matsuda, M.: "Substrate specificity of enzymes oxidizing 4-aminobutyraldehyde to GABA in rat brain" Jikeikai Med. J.36 :. 317-325 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Asakura, T., Ikeda, Y., & Matsuda, M.: "Distribution of activity converting 4-aminobutyraldehyde to GABA in subcellular fractions of mouse brain" J. Neurochem.52. 448-452 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Asakura, T., & Matsuda, M.: "Effect of gabaculine on metabolism and release of gamma-aminobutyric acid in synaptosomes" Neurochem. Res.15. 295-300 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Abe, T.: "Purification and charcterization of a rat aldehyde dehyrogenase able to metabolize γーaminobutyraldehyde to γーaminobutyric acid" Biochemical J.269. 25-29 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Asakura, T.: "Effect of gabaculine on metabolism and release of γーaminobutyric acid in synaptsome" Neurochem. Res.15. 295-300 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 阿部 俊夫: "鶏胚脳発生とγーアミノブチルアルデヒド脱水素酵素活性" 医学のあゆみ. 155. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Abe,T.: "Purification and characterization of a rat brain aldehyde dehydrogenase able to metabolize γ-aminobutyraldehyde to γ-aminobutyric acid" Biochemical J.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takada,K.: "Substrate specificity of enzymes oxidizing 4-aminobutyraldehyde to GABA in rat brain" Jikeikai Med.J.36. 317-325 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Asakura,T.: "Distribution of activity converting 4-aminobutyraldehyde to GABA in subcellular fractions of mouse brain" J.Neurochem.52. 448-452 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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