| Project/Area Number |
01570193
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Hiroshima University |
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Principal Investigator |
KAMIYA Kenji Research Associate Department of Pathology, Research Institute for Nuclear Medicine and Biology, Hiroshima University, 原爆放射能医学研究所, 助手 (60116564)
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| Co-Investigator(Kenkyū-buntansha) |
YOKORO Kenjiro Professor Department of Pathology, Research Institute for Nuclear Medicine and B, 原爆放射能医学研究所, 教授 (70034618)
NIWA Ohtsura Associate Professor Department of Pathology, Research Institute for Nuclear Medi, 原爆放射能医学研究所, 助教授 (80093293)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Rat mammary epithelial cell line / Rat pituitary cell line / Autocrine growth factor / Glucocorticoid / Chemically defined medium / 成長因子 / 乳腺上皮細胞 / 下垂体由来増殖因子 / 乳腺由来増殖因子 |
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| Research Abstract |
Normal rat mammary epithelial cell line was established from continuous cultures of rat mammary cells in chemically defined medium. These cells were shown to be mammary epithelial origin by in vivo transplantation assay, electron microscopic examination and immunocytochemical analysis. Using these mammary cells as target cells, in vitro bioassay system for the detection of the mitogenic activity on mammary cells was developed.
Four rat pituitary cell lines, derived from mammotropic pituitary tumors (MtT-F4, MtT-F84), and radiation induced somatomammotropic pituitary tumors (PT1,PT3), were also established in chemically defined medium. As the results of in vitro analysis of mitogenic activity of conditioned medium from established pituitary cell cultures, conditioned medium from PT3 was shown to have strong growth-promoting effects on mammary cells. cc152 cells, isolated from PT3, were found to have unique growth characteristics that cc152 cells could proliferate in the chemically defined medium supplemented with insslin and hydrocortisone without any other growth-promoting factors. Furthermore, cc152-c3 cells derived from cc152 were able to proliferate in the chemically defined medium supplemented with hydrocortisone alone. Conditioned medium from cc152-c3 cells stimulated the growth of their own cells. The growth-stimulating effects of hydrocortisone on cc152 and cc152-c3 cells was inhibited by the addition of a glucocorticoid receptor antagonist, RV486. RV486 also blocked hydrocortisone-induced growth-stimulating effects of conditioned medium. These results indicated that stimulation of cc152-c3 cells with hydrocortisone resulted in secretion of an autocrine growth factor (s).
A preliminary characterization of the growth factor (s) in the conditioned medium suggested that growth factor (s) was a polypeptide. Experiments are being carried out to purify and further characterize the glucocorticoid-induced growth factor (s).
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