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Mechanism of Amyloid Fibril Formation and Resorption - A Protein A-Gold Immunoelectron Microscopic Study -

Research Project

Project/Area Number 01570195
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionYamaguchi University

Principal Investigator

TAKAHASHI Mutsuo  Yamaguchi University Clinical Laboratories Associate Professor, 医学部, 助教授 (50112230)

Co-Investigator(Kenkyū-buntansha) UCHINO Fumiya  Yamaguchi University Pathology Professor, 医学部, 教授 (20034902)
ISHIHARA Tokuhiro  Yamaguchi University Pathology Associate Professor, 医学部, 助教授 (70089910)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsAmyloidosis / Kupffer cell / Immunoelectron microscopy / Intracellular formation / アミロイド-シス / kupffer細胞
Research Abstract

Amyloid formation and resorption in the murine liver were examined by the technique of protein A-gold immunoelectron microscopy using anti-mouse amyloid antiserum. The liver biopsy was done 4 days after the injections of amyloid enhancing factor and casein solution, and the mice were sacrificed 1 an 2 weeks after the biopsy.
In the biopsied liver, amyloid fibrils were seen mainly in the Disse's space along the cytoplasmic invaginations of Kupffer cells. By immunoelectron microscopy, gold particles labeled several inclusions within the cytoplasm of the Kupffer cells. These inclusions, being regularly round to fusiform in shape and measuring 0.5-1.0 mum in width and 1.0-3.0 mum in length, were constantly membrane-bounded. Some of them contained fibrillar structures, corresponding to amyloid fibrils, while most were composed of a homogeneous, granular matrix. These inclusions were thought to be derived from lysosomes or phagolysosomes based on their ultrastructural configurations. In the stage of amyloid resorption, gold particles labeled irregularly-shaped heterophagosomes in the Kupffer cells. These heterophagosomes contained flocculent and phagocytized membranous structures and amyloid fibrils as well, showing various stages of digestion.
The present results provide an additional support for the concept that some amyloid fibrils are formed within lysosome-derived organelles of the Kupffer cells following proteolytic cleavage of pinocytized serum amyloid A protein (a precursor protein of secondary amyloidosis).

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] M.Takahashi,et al.: "Ultrastructural evidence for intracellular formation of amyloid dibrils in macrophages." Virchows Arch A. 415. 411-419 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takahashi,et al:"A comparative study of amyloid formation and resorption using immunoelectron microscopy." In Amyloidosis ed by J.B.Natvig,et al.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takahashi: "Intracelluar formation of amyloid fibrils by reticuloendothelial cells ーAn immunoelectron microscopic studyー" J Clin Electron Microscopy.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takahashi, T. Yokota, T. Ishihara, H. Kawano, T. Gondo, Y. Yamashita, F. Uchino and T. Iwata: "A comparative study of amyloid formation and resorption using immunoelectron microscopy" In Amyloidosis ed. by J. B. Natvig, et al.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takahashi: "Intracellular formation of amyloid ribrils by reticuloendothelial cells - An immunoelectron microscopic study -" J Clin Electron Microscopy.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Takahashi, T. Yokota, H. Kawano, T. Gondo, T. Ishihara, and F. Uchino: "Ultrastructural evidence for intracellular formation of amyloid fibrils in macrophages" Virchows Arch A. 415. 411-419 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Takahashi,et al.: "Ultrastructural evidence for intracellular formation of amyloid fibrils in macrophages." Virchows Archiv A. 415. 411-419 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Takahashi,et al.: "A comparative study of amyloid formation and resorption using immunoelectron microscopy." In Amyloidosis ed.by J.B.Natvig,et al.

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Takahashi: "Intracellular formation of amyloid fibrils by reticuloendーothelial cells ーAn immunoelectron microscopic studyー" J.Clin Electron Microscopy.

    • Related Report
      1990 Annual Research Report
  • [Publications] Mutsuo Takahashi,et al.: "Ultrastructural evidence for intracellular formation of amyloid fibrils in macrophages" Virchows Archiv A Pathological Anatomy and Histopathology. 415. 411-419 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mutsuo Takahashi,et al.: "Intracellular formation of amyloid fibrils in Kupffer cells" Amyloidosis.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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