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Augmentation of antitumor activity by Trypanosome derived substances.

Research Project

Project/Area Number 01570226
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionHyogo College of Medicine

Principal Investigator

SHINKA Souhei  Hyogo College of Medicine, Dept. of Immunoloy and Medical Zoology, Professor, 医学部, 教授 (10029770)

Co-Investigator(Kenkyū-buntansha) YAMADA Kazuhiko  Hyogo College of Medicine, Dept. of Immunology and Medical Zoology, Assistant fe, 医学部, 助手 (60220365)
MIYAMOTO Hikosiro  Hyogo College of Medicine, Dept. of Immunology and Medical Zoology, Assistant fe, 医学部, 助手 (90175620)
KOMATSU Tosinori  Hyogo College of Medicine, Dept. of Immunology and Medical Zoology, Lecturer, 医学部, 講師 (40131578)
FUKUMA Tosihide  Kurume University, School of Medicine, Dept. of Parasitology, Professor, 医学部, 教授 (90125146)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
KeywordsTrypanosoma brucei / Culture Supernatant / IFN / NK cell activity / mice / serum / トリパノゾ-マ原虫 / 感染血清 / 虫体ホモジネイト / インタ-フェロンα / β / Trypanosoma brucei gambiense / 抗腫瘍活性
Research Abstract

Although it is known that interferon (IFM) are released into serum during experimental African Trypanascmiasis in mice, there is no report indicating that solube component released fran the parasite into the culture supernatant (CS) is able to induce IFN activity in mouse serum. In this study, we found first that administration of CS of Trypanoscma brucei gambiense (Tg) to mice results in transient induction of IFN alpha/beta in serum 24 to 48h after injection and augmentation of splenic natural killer activity against YAC-1 lymphoma cell 1 day later in similar kinetics as the infection with the parasite.
While the degree and kinetics of serum IEN induction by CS in 3 inbred mice were almost same, augmentation profit of NK activity was different among them indicating the participation of genetic control in it.
CS also exerts influence on immune responses of mice as administered at the time of immunization with specific antigens, that is, IgE antibody. response to hen egg lysozyme and con … More tact sensitivity to DNFB were suppressed while IGG/M antibody responses to the lysozyme and sheep red blood cell. Blastogenesis of spleen cell by LPS or Con A was not affected by addition of CS.
However, as the yield and stability of the IFN inducing activity in CS were considerably poor, it seemed to be difficult to isolate and analyze the active canponent in CS. Then, the serun of Tg infected mice which was shown to have a very high and stable ability to induce IFN in similar manner as CS was subjected to further studies and it was shown that 1) the activity of the serum is transferable "M mice but not to rats. 2) the activity is inactivated by heat treatment or trypsin digestion and precipitable in 50-70% anmnium sulface. 3) Acoording to the molecular size, it is devided to two fractions, one is low molecular (MW. ca2OOO) and oher is high molecular (mw. calo). Moreover, it is speculated that the activity in the serum is derived f rom parasite infected host itself as similar low molecular fraction separated fran CS or Tg homogenate did not show such IEN inducing activity. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 福間 利英: "原虫のもつ免疫回避機構の分子生物学" Medical immunoligy. 22ー5. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 宮本 彦四郎他3名: "ヒト及びモルモット血清中に認められる殺トリパノゾ-マ成分の検討" 長崎大学熱帯医学研究所 共同研究報告集. 31-33 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 宮本 彦四郎他1名: "トリバノゾ-マ原虫に対するNK細胞" 医学のあゆみ. 152ー7. 436 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 福間 利英: "熱帯原虫病の分子生物学" 遺伝. 44ー8. 37-42 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新家 荘平他1名: "トリパノゾ-マの抗原変異" 遺伝. 143ー4. 39-42 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 新家 荘平他5名: "Trypanosoma brucei gambiense感染マウス血清中の1Lー2の産生抑制物質" 日本熱帯医学会雑誌. 17. 9-15 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 福間 利英: "原虫のもつ免疫回避機構の分子生物学" Medical immunoligy. 22ー5. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 宮本 彦四郎他3名: "ヒト及びモルモット血清中に認められる殺トリパノゾ-マ成分の検討" 長崎大学熱帯医学研究所共同研究報告集. 31-33 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 宮本 彦四郎他1名: "トリパノゾ-マ原虫に対するNK細胞" 医学のあゆみ. 152ー7. 436 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 福間 利英: "熱帯原虫病の分子生物学" 遺伝. 44ー8. 37-42 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 新家 荘平他1名: "トリパノゾ-マの抗原変異" 遺伝. 143ー4. 39-42 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 新家 荘平他5名: "Trypanosoma brucei gambiense感染マウス血清中のILー2産生抑制物質" 日本熱帯医学会雑誌. 17. 9-15 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 新家 荘平: "トリパノゾ-マの抗原変異" 遺伝. 43. 39-42 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 福間 利英: "ヒトおよびモルモット血清中に認められる殺トリパノゾ-マ成分の検討" 長崎大学熱帯医学研究所共同研究報告集. 31-33 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 宮本 彦四郎: "トリバノゾ-マ原虫に対するNK細胞" 遺伝. 152. 436 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 福間 利英: "熱帯原虫病の分子生物学" 遺伝. 44. 31-33 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshihide Fukuma: "Simple and effective measure on initiative cultivation of Trypanosoma b.gambiense in vitro." Tropical Medicine. 33. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshihide Fukuma: "Is serum albumin toxic for bloodstream forms of African Trypanosomes in vitro?" Tropical Medicine. 33. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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