| Project/Area Number |
01570253
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Kanazawa University, School of Medicine |
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Principal Investigator |
OGURA Hisashi Kanazawa University, School of Medicine, Associate Professor, 医学部, 助教授 (10115222)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Hiroshi Kanazawa University, Cancer Research Institute, Research Associate, がん研究所, 助手 (00115239)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Measles Virus / M Protein / Elevated Temperatures / Gene Transfer / Transient Expression / 麻疹ウィルス |
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| Research Abstract |
We analyzed a phenomenon that selective translational inhibition of measles virus Membrane (M) protein synthesis in the infected cells at elevated temperatures by transfection of measles virus fullーlength cDNA clones. We used four kinds of eukaryotic expression vectors for transient expression of measles virus Hemagglutinin (H) and M proteins. Among them, only pSG5 vector under the control of the SV40 early promoter was expressible. H protein was well expressed in the H cDNA-transfected COS-1 cells at both 35^ﾟC and 39^ﾟC. On the other hand, although M protein was expressed in the M cDNA-transfected cells at 35^ﾟC, its synthesis became undetected immediately after shiftーup to 39^ﾟC in spite of the presence of the M mRNA. Thus, it was demonstrated that the selective translational inhibition of M protein synthesis is reprosuced in the M cDNA-transfected cells by temperature elevation in the absence of the other MV specific RNAs and proteins.
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