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Immunogenicity, Pathogenicity, and Epidemiological Application of Rotavirus Protective Antigens

Research Project

Project/Area Number 01570256
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionSchool of Medicine, Sapporo Medical College

Principal Investigator

TANIGUCHI Koki  School of Medicine, Sapporo Medical College, Assistant Professor, 医学部, 講師 (40094213)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Nobumichi  School of Medicine, Sapporo Medical College, Instructor, 医学部, 助手 (80186759)
URASAWA Tomoko  School of Allied Health Professions, Sapporo Medical College, Professor, 衛生短大部, 教授 (90045378)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsRotavirus / Plaque Size / Antibody Response / VP4 / VP7 / RNA-RNA Hybridization / Serotype / VP4 / VP7 / ハイブリダイゼ-ション / 病原性
Research Abstract

1. Nucleotide Sequence determination of the 4th RNA segment of SA11 clones with different plaque size identified five amino acids difference between large (L2) and small (S1) clones. In the mice administered orally with L2 clone, incubation time for diarrhea was shorter and the degree of diarrhea was severer than in those infected with S1 clone.
2. In a competitive immunoassay using serotype-specific and cross-reactive neutralizing monoclonal antibodies directed at VP4 and VP7, antibody response to VP7 epitopes of the infecting serotype of the virus was found in both infants and children in a high frequency. In contrast, antibody response to VP4 and to heterotypic VP7 was observed only when the individuals possessed antibodies to any serotype of rotavirus in their acute-phase or pre-vaccination sera.
3. We sequenced the genes coding for VP4 and VP7 of human rotavirus strains L26 and L27 with subgroup I specificity but the long RNA pattern. The deduced VP7 amino acid sequence of strains L … More 26 and L27 showed a low homology (73.6 to 81.9%) to those of rotavirus strains of the established serotypes. This finding, together with the previous serological characterizations, suggests that the VP7 (G) serotype of the L26 and L27 strains is distinct from those of strains of the previously established serotypes. In contrast, the VP4 sequences of the L26 and L27 strains were quite similar to those of virulent serotype 2 strains. RNA-RNA hybridization tests indicated an overall genetical relationship of the L26 and L27 strains with serotype 2 human strains.
4. Antigenic and genetic properties of non-serotype 6 bovine rotaviruses isolated in Thailand were studied by cross-neutralization tests, nucleotide sequence determination of VP7 gene, and RNA-RNA hybridization. Two strains (61A and A44) were serologically and genetically related to strain KK3 of serotype 10. In contrast, A5 strain was found to be antigenically similar to human serotype 8 strain 69M. VP7 sequence analysis confirmed this finding. These bovine strains exhibited a moderate overall genetic relatedness with serotype 2 and serotype 8 human rotavirus strains. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Shozo Urasawa et al.: "Presumptive seventh serotype of human rotavirus" Archives of Virology. 113. 279-282 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koki Taniguchi et al.: "Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern:Implication for new G serotype specificity" Journal of Virology. 64. 5640-5644 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koki Taniguchi et al.: "Nucleotide sequence of the VP7 gene of a bovine rotavirus(strain61A)with different serotype specificity from serotype 6" Nucleic Acids Research. 18. 4613 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koki Taniguchi et al.: "Antibody response to serotype‐speific and cross‐reactive neutralization epitopes on VP4 and VP7 after rotavirus infection or vaccination" Journal of Clinical Microbiology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koki taniguchi et al.: "Association of plaque size and VP4 sequence of SA11 virus clones" Archives of Virology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koki Taniguchi et al.: "Molecular and antigenic analyses of non‐serotype 6 bovine rotaviruses:Evidence for the prevalence of serotype 8 and 10 bovine strains in THailand" Virology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, N., Lintag, I. C., Urasawa, T., Taniguchi, K., Saniel, M. C., and Urasawa, S.: "Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype." Arch. Virol. 109. 11-24 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Urasawa, S. Urasawa, T. Wakasugi, F., Kobayashi, N., Taniguchi, K., Lintag, I. C., Saniel, M. C., and Goto, H.: "Presumptive seventh serotype of human rotavirus." Arch. Virol. 113. 279-282 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, K., Urasawa, T., Kobayashi, N., Gorziglia, M., and Urasawa, S.: "Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern : Implication for new G serotype specificity" J. Virol.64. 5640-5644 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, K., Pongsuwanna, Y., Choonthanom, M., and Urasawa, S.: "Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6." Nucleic Acids Res.18. 4614 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, N., Taniguchi, K., and Urasawa, S.: "Identification of operationally overlapping and independent cross-reactive neutralization regions on human rotavirus VP4." J. Gen. Virol.71. 2615-2623 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kobayashi, N., Taniguchi, K., and Urasawa, S.: "Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1." J. Gen. Virol.72. 117-124 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Urasawa, T., Taniguchi, K., Kobayashi, N., Wakasugi, F., Oishi, I., Minekawa, Y., Oseto, M., Ahmed, M. U., and Urasawa, S.: "Antigenic and genetic analyses of human rotavirus with dual subgroup specificity." J. Clin. Microbiol.28. 2837-2841 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Pongsuwanna, Y., Taniguchi, K., Choonthanom, M., Chiwakul, Malliga, Jayavasu, Chuinrudee, Snodgrass, D., and Urasawa, S.: "Serological and genetical characterization of bovine rotaviruses in Thailand by ELISA and RNA-RNA hybridization : Detection of numerous non-serotype 6 strains." Southeast Asian J. Trop. Med. Publ. Health.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, K., urasawa, T., Kobayashi, N., Ahmed, M. U., Adachi, N., Chiba, S., and Urasawa, S.: "Antibody response to serotype-specific and cross-reactive neutralization epitopes on VP4 and VP7 after rotavirus infection or vaccination." J. Clin. Microbiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, K., Nishikawa, K., Kobayashi, N., Urasawa, T., Gorziglia, M., Urasawa, S.: "Association of plaque size with VP4 sequence of SA11 virus clones." Arch. Virol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taniguchi, K., Urasawa, T., Pongsuwanna, Y., Choonthanom, M., Jayavasu, C., and Urasawa, T.: "Molecular and antigenic analyses of non-serotype 6 bovine rotaviruses : Evidence for the prevalence of serotype 8 and 10 bovine strains in Thailand." Virology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shozo Urasawa et al.: "Presumptive seventh serotype of human rotavirus" Archives of Virology. 113. 279-282 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup I specificity and long RNA pattern:Implication for new G serotype specificity" Journal of Virology. 64. 5640-5644 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6" Nucleic Acids Research. 18. 4613- (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yaowapa Pongsuwanna et al.: "Serological and genetical characterizations of bovine rotaviruses in Thailand by ELISA and RNAーRNA hybridization:Detectin of numerous nonーserotype 6 strains" Southeast Asian Journal of Tropical Medicine and Public Health.

    • Related Report
      1990 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Association of plaque size with VP4 sequence of SA11 virus clones" Archives of Virology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Molecular and antigenic analyses of nonーserotype 6 bovine rotaviruses:Evidence for the prevalence of serotype 8 and 10 bovine strains in THailand" Virology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Muzahed Uddin Ahmed et al.: "Characterization by enzyme-linked immunosorbent assay using subgroup-and serotype-specific monoclonal anti-bodies of human rotavirus obtained from diararheic patients in Bangladesh" Journal of Clinical Microbiology. 27. 1678-1681 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yaowapa Pongsuwanne et al.: "Subgroup and serotype distributions of human,bovine,and porcine rotavirus in Thailand" Journal of Clinical Microbiology. 27. 1956-1960 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Complete nucleotide sequence of the gene encoding VP4 of a human rotavirus(strain K8)which has unique VP4 neutralization epitopes" Journal of Virology. 63. 4101-4106 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nobumichi Kobayashi et al.: "Unusual human rotavirus strains having subgroup I specificity and “long" RNA electropherotype" Archives of Virology. 109. 11-23 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Antibody response to the serotype-specific and cross-reactive neutralization epitopes on VP4 and VP7 after rotavirus infection or vaccination" Journal of Clinical Microbiology.

    • Related Report
      1989 Annual Research Report
  • [Publications] Koki Taniguchi et al.: "Nucleotide sequence analysis of VP4 and VP7 genes of strains L26 and L27 having subgroup I specificity and long RNA pattern" Journal of Virology.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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