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Study on the IL-1-Mediated Signal Transduction System

Research Project

Project/Area Number 01570273
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionJichi Medical School

Principal Investigator

KASAHARO Tadashi  Jichi Medical School, Associate Prof., 医学部, 助教授 (60049096)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Yuji  Jichi Medical School, Assistant, 医学部, 助手 (00220286)
ONOZAKI Kikuo  Nagoya City Univ. Dept. Pharmaceu. Sci. Prof., 薬学部, 教授 (20101313)
YAGISAWA Hitoshi  Jichi Medical School, Instructor, 医学部, 講師 (40192380)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsIL-1 / cAMP / IL-6 / IL-8 / astrocytes / signal transduction / IL1 / IL6 / コレラトキシン
Research Abstract

IL-1 has a variety of functions not only in the immune response but also in neuroendocrine system, affecting proliferation, differentiation and inducing inflammatory mediators. Although IL-1 receptor and its antagonist cDNAs have been cloned, IL-1-mediated signal transduction system has not yet been clarified. In these regards, we have done following studies :
(1) Role of IL-1 and its action have been investigated in the human T cell leukemia cell line, HSB. 2 cells, particularly for the synergistic action of IL-1 in the induction of IL-2 and IFN-r mRNA production (Microbiol. Immunol. 1989 ; Immunol. 1990).
(2) We have demonstrated that human astrocytoma cell line, U373MG, responds to IL-1 with high level production of IL-6. The action of IL-1 was not mediated by cAMP, but was augmented by cAMP, showing modulatory role of cAMP in this system (B. B. R. C. 1990).
(3) In addition, U373 cells produce high levels of IL-8 and MCAF (Mo chemotactic and activating factor) mRNA expression in response to IL-1. Transcriptional regulation of these genes by IL-1 and stabilization have been studied in this system as well as several hepatoma cell lines (manuscript submitted).
(4) In relation to the cytokine network, role and action of other cytokines (IL-2, 4 and 5), particularly in the context of B cell activation and parasites-induced eosinophila have been studied (J. Immunol. 1990 ; Exp. Hematol. 1990 ; Life Science Adv. in press).
We believe that these studies contribute very much to the understanding of role and action of cytokines in vitro and in vivo, and to the manipulation of inflammatory diseases including infection, autoimmune and neoplastic diseases.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (50 results)

All Other

All Publications (50 results)

  • [Publications] Kashara,T.,Yagisawa,H.,Mukaida,N.,ほか: "IL1 regulation of IL2 and IFNーγ gene activation in a human leukemic HSB.2 HSB.2 subclone." Microbiol.Immunol.33. 229-243 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyagi,J.,Minato,N.,Sumiya,M.,Kasahara,T.,Kano,S.: "Two types of antibodies innibiting IL2 production by normallymhocytes in patients with systemic lipus ery thematoosus." Arthritis Rheumat.32. 1356-1364 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara,T.,Yagisawa,H.,Yamashita,k.,Yamaguchi,Y.Akiyama,Y.: "IL1 induces proliferation and IL6 mRNA expression in a human astrocytoma cell line:positive and negative modulation by chorela toxin and cAMP." Biochem.Biophys.Res.Commun.167. 1242-1248 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsunoda,H.,Tamatani,T.,Oomoto,Y.,Hirai,Y.,Kasahara,T.,ほか: "Changes in IL1 levels in amniotic fluid with gestational ages and delivery." Microbiol.Immunol.34. 377-385 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamaguchi,Y.,Suda,T.,Shiozak,H.,Kasahara,T.,ほか: "In vivo and in vitro induction of IL5 mRNA by IL2 that causes eosinophilia in mice." J.Immunol.145. 873-877 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamaguchi,Y.,Matsui,T.,Kasahara,T.,ほか: "In vivo changes of hematopoietic progenitors and the ecpression of ILー5 gene in eosinophilic mice inTected with Toxocara canis." Exp,Hematol.18. 1152-1157 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yagisawa,H.,Kasahara,T.,Mukaida,N.,ほか: "ILー1ーinduced production of ILー2 and IFNーγ in subclones of human T cell cellーderived leukemia HSB.2 cels:regulation by PHAーmediated(poly)phosphoinasitide breakdown and cーAMP." Immunology. 71. 242-250 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamashita,K.,Kasahara,T.,Okazaki,H.,ほか: "Effect of various interleukins on human B cell activation:analysis of inhibitory action of ILー4." Life Science Avances.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara,T.,mukaida,N.,Yamashita,k.,ほか: "ILー1ーinduction of ILー8 and monocyte chemotactic and activating factor(MCAF)mRNA expression in a human astorocytoma cell."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 八木沢 仁,笠原 忠: "ILー1リセプタ-" 臨床免疫. 21. 1665-1676 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 笠原 忠,赤星 透,角田肇,小野崎 菊夫: "炎症反応と臨床検査ーインタ-ロイキン1" 臨床病理. 38. 243-248 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 笠原 忠,寺田 泰比古: "造血機構とその調節因子1ー4.IL1の作用の機構(実験医学 臨時増刊号)" 羊土社, 204 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 芳郎,笠原 忠: "新生化学実験講座 第7巻増殖分化因子とその受容体1.3.2. インタ-ロイキン1" 東京化学同人(日本生化学会編),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 笠原 忠: "Current review 臨床検査1990ー1991(4.サイトカイン)" 中外医学社, 6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 笠原 忠,向田 直史: "蛋白質・核酸・酵素 臨時増刊号「細胞増殖因子の基礎と臨床」(1Lー1の産生と作用の機構)" 共立出版, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara, T., Yagisawa, H., Mukaida, N. et al.: "Interleukin 1 regulation of interleukin 2 and interferon-gamma gene activation in a human leukemic HSB. 2 subclone." Microbiol. Immunol.33. 229-243 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyagi, J. Minato, N., Sumiya, M. et al.: "Two types of antibodies inhibiting interlekin-2 production by normal lymphocytes in patients with systemic lupus erythematosus." Arthritis Rheumat. 32. 1356-1364 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara, T., Yagisawa, H., Yamashita, K. et al.: "IL-1 induces proliferation and IL-6 mRNA expression in a human atrocytoma cell line : Positive and negative modulation by chorela toxin and cAMP." B. B. R. C.167. 1242-1248 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yagisawa, H., Kasahara, T., Mukaida, N. et al.: "IL-1-induced production of IL-2 and IFN-gamma in subclones of human T cell derived leukemia HSB. 2 cells : regulation by PHA-mediated (poly)phosphoinositide breakdown and cyclic AMP." Immunology. 71. 242-250 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsunoda, H., Tamatani, T., Oomoto, Y. et al.: "Changes in interleukin 1 levels in human amniotic fluid with gestational ages and delivery." Microbiol. Immunol.34. 377-385 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Suzu, S., Yanai, N., Kawashima, T. et al.: "Enhancement of the antibody-dependent tumoricidal activity of human monocytes by human M-CSF." Jap. J. Cancer Res.81. 79-84 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamaguchi, Y., Suda, T., Shiozaki, H. et al.: "In vivo and in vitro induction of IL-5 mRNA by IL-2 that causes eosinophilia in mice." J. Immunol.145. 873-877 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamaguchi, Y. Matsui, T., Kasahara, T. et al.: "In vivo changes of hematopoietic progenitors and the expression of IL-5 gene in eosinophilic mice infected with Toxocara canis." Exp. Hematol.18. 1152-1157 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamashita, K., Kasahara, T., Okazaki, H. et al.: "Effect of various interleukins on human B cell activation : analysis of inhibitory action of IL-4." Life Science Adv.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara T., Mukaida, N., Yamashita, K. et al.: "IL-1 induction of IL-8 and monocyte chemotactic and activating factor (MCAF) mRNA expression in a human astrocytoma cell line."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yagisawa H. & Kasahara, T.: "IL-1 receptor." Clin. Immunol.21. 1665-1676 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara, T., Akahosi, T., Tsunoda, H. et al.: "Interleukin-1." Jpn. J. Clin. Pathol.38. 243-248 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara,T.,Yagisawa,H.,Mukaida,N.,ほか: "IL1 regulation of IL2 and IFNーγ gene activation in a human leukemic HSB.2 subclone." Microbiol.Immunol.33. 229-243 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyagi,J.,Minato,N.,Sumiya,M.,Kasahara,T.,Kano,S.: "Two types of sntibodies anhibiting IL2 production by normal lymphocytes in patients with systemic lupus ery thematosus." Arthitis Rheumat.32. 1356-1364 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kasahara,T.,Yagisawa,H.,Yamashita,K.,Yamaguchi,Y.Akiyama,Y.: "IL1 induces proliferation and IL6 mRNA expression in a human astrocytoma cell line:Positive and negative modulation by chorela toxin and cAMP." Biochem.Biophys.Res.Commun.167. 1242-1248 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tsunoda,H.,Tamatani,T.,Oomoto,Y.,Hirai,Y.,Kasahara,T.,ほか: "Changes in IL1 levels in amniotic fluid with gestational ages and delivery." Microbiol.Immunol.34. 377-385 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamaguchi,Y.,Suda,T.,Shiozaki,H.,Kasahara,T.,ほか: "In vivo and in vitro induction of IL5 mRNA by IL2 that causes eosinophilia in mice." J.Immunol.145. 873-877 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamaguchi,Y.,Matsui,T.,Kasahara,T.,ほか: "In vivo changes of hematopoietic progenitors and the ecpression of ILー5 gene in eosinophilic mice intected with Toxocara canis." Exp.Hematol.18. 1152-1157 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yagisawa,H.,Kasahara,T.,Mukaida,N.,ほか: "ILー1ーinduced production of ILー2 and IFNーγ in subclones of human T cellーderived leukemia HSB.2 cells:regulation by PHAーmediated (poly)phosphoinasitide breakdown and cーAMP." Immunology. 71. 242-250 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamashita,K.,Kasahara,T.,Okazaki,H.,ほか: "Effect of various interleukins on human B cell activation:analysis of inhibitory action of ILー4." Life Science Advances.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kaahara,T.,mukaida,N.,Yamashita,K.,ほか: "ILー1ーinduction of ILー8 and monocyte chemotactic and activating factor(MCAF)mRNA expression in a human astorocytoma cell."

    • Related Report
      1990 Annual Research Report
  • [Publications] 八木沢 仁,笠原 忠: "ILー1リセプタ-" 臨床免疫. 21. 1665-1676 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 笠原 忠,赤星 透,角田 肇,小野崎 菊夫: "炎症反応と臨床検査ーインタ-ロイキン1" 臨床病理. 38. 243-248 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 笠原 忠,寺田 泰比古: "造血機構とその調節因子Iー4.IL1の作用の機講 (実験医学 臨時増刊号) 204頁のうちの6頁" 羊土社, 6 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 芳郎,笠原 忠: "新生化学実験講座 第7巻増殖分化因子とその受容体 1.3.2. インタ-ロイキン1" 東京化学同人(日本生化学会編),

    • Related Report
      1990 Annual Research Report
  • [Publications] 笠原 忠: "Current review 臨床検査1990ー1991(4.サイトカイン)" 中外医学社, 6 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 笠原 忠,向田 直史: "蛋白質・核酸・酵素 臨時増刊号「細胞増殖因子の基礎と臨床」(ILー1の産生と作用の機構)" 共立出版, 9 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kasahara,T.,Yagisawa,H.,Mukaida,N.,ほか: "IL1 regulation of IL2 and IFN-γ gene activation in a human leukemic HSB.2 subclone." Microbiol.Immunol.33. 229-243 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyagi,J.,Minato,N.,Sumiya,M.,Kasahara,T.,Kano,S.: "Two types of antibodies inhibiting IL2 production by normal lymphocytes in patients with systemic lupus ery thematosus." Arthritis Rheumat.32. 1356-1364 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kasahara,T.,Yagisawa,H.,Yamashita,K.,Yamaguchi,Y.Akiyama,Y.: "IL1 induces proliferation and IL6 mRNA expression in a human astrocytoma cell line:Positive and negative modulation by chorela toxin and cAMP." Biochem.Biophys.Res.Commun.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsunoda,H.,Tamatani,T.,Oomoto,Y.,Hirai,Y.,Kasahara,T.,ほか: "Changes in IL1 levels in amniotic fluid with gestational ages and delivery"

    • Related Report
      1989 Annual Research Report
  • [Publications] Yamaguchi,Y.,Suda,T.,Shiozaki,H.,kasahara,T.,ほか: "In vivo and in vitro induction of IL5 mRNA by IL2 that causes eosinophillia in mice."

    • Related Report
      1989 Annual Research Report
  • [Publications] 笠原忠,赤星透,角田肇,小野崎菊夫ほか: "炎症反応と臨床検査一インタ-ロイキン1" 臨床病理.

    • Related Report
      1989 Annual Research Report
  • [Publications] 笠原忠,寺田泰比古: "造血機構とその調節因子1-4.IL1の作用の機構(実験医学 臨時増刊号)" 羊土社, 6 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林芳郎,笠原忠: "新生化学実験講座 第7巻増殖分化因子とその受容体1.3.2.インタ-ロイキン1" 東京化学同人(日本生化学会編),

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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