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The new biological estimation for air pollution, indoor pollution and working environment

Research Project

Project/Area Number 01570304
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionUniversity of Occupational and Environmental Healt

Principal Investigator

KODAMA Yasushi  University of Occupational and Environmental Health, Sch Med, Professor, 医学部, 教授 (20037360)

Co-Investigator(Kenkyū-buntansha) ARASHIDANI Keiichi  University of Occupational and Environmental Health, Sch Nurs Med Tech, Assoc Pr, 医療技術短期大学, 助教授 (10141748)
KAYAMA Fujio  University of Occupational and Environmental Health, Sch Med, Associate Professo, 医学部, 助手 (50177614)
MATSUNO Koji  University of Occupational and Environmental Health, Sch Med, Associate Professo, 医学部, 助手 (40131940)
KAWAMOTO Toshihiro  University of Occupational and Environmental Health, Sch Med, Associate Professo, 医学部, 助教授 (60177748)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
KeywordsAir Pollution / Indoor Pollution / EGF / Cytochrome P-450 / Nitrogen Dioxide / AHH / Cigarette Smoke / PAH / イソ酵素 / Hela細胞
Research Abstract

In this research project, we studied new biological indicators which were useful to assess the risk of chronic obstructive lung diseases and cancer promoting action by air pollution, indoor pollution and working environment. The biological effects of main pollutans, i. e. cigarette smoke, nitorgen dioxide (NO_2), benzo[a]pyrene (BaP), etc, were investigated in vivo and in vitro. Rats were exposed to side stream of cigarette smoke and NO_2 in an experimental chamber, 8 hours/day for 5 days. The activities of aryl hydrocarbon hydroxylase of lung, kidney and liver were increased by the exposure to cigarette smoke, but not by NO_2. Though total contents of cytochrome P-450 were not induced by either cigarette smoke or NO@_2, the dose-dependent inductions of cytochrome P-450b, c, d isozymes by the cigarette smoke wererecognized using Western Immunoblot analysis. The protein concentration, alkaline phosphatase and lactate dehydrogenase acticities in the bronchoalveolar lavage fluid (BALF) were also increased in the rats exposed to cigarette smoke or NO_2. The change of BALF was the most sensitive indicator that we examined in this study. The binding assay of epidermal growth factor (EGF) was also performed using HeLa cell and C3H/1OT1/2 cell. The binding of EGF to EGF receptor reached at a plateau 60-80 min after starting the incubation. This binding was varied by the density of cells on the plate. The plate with low density of the cells shows high binding capacity per cell number. We examined the effect of BaP on the EGF binding in the condition of the same density of the cells, however, it was not recognized that BaP inhibits EGF binding. In this experiment, some biological indicators did not show the result that we had expected. Further investigations were required to clarify the reason.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Masahiro Yoshikawa,Keiichi Arachidani,Toshihiro Kawamoti,Yasushi Kodama: "Organ specificity of aryl hydrocarbon hydroxylase induction by cigarette smoke" Bulletin of Environmental Contamination and Toxicology. 44. 940-947 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 土屋 健三郎,児玉 泰他: "地球環境問題から室内汚染までーリスクアセスメントー" 産業医科大学雑誌. 12. 457-461 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小山 倫浩,川本 俊弘,児玉 泰他: "二酸化窒素およびタバコ煙曝露による肺胞洗浄液の変化" 日本衛生学雑誌. 45. 363 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 平井 学,川本 俊弘,児玉 泰他: "大気汚染物質による上皮成長因子の細胞表面受容体への結合抑制について" 産業医学. 32. 715 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahiro Yoshikawa, Keiichi Arashidani, Toshihiro Kawamoto, Yasushi Kodama: "Organ specifificity of aryl hydrocabonhydroxylase induction by cigarette smoke" Bulletin of Environmental Contamination and Toxicology. 44. 940-947 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kenzaburo Tsuchiya, Yasushi Kodama et al.: "From global environment to indoor pollution-Risk assessment-" Journal of UOEH. 12. 457-461 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsunehiro Oyama, Toshihiro Kawamoto, Yasushi Kodama et al.: "The changes of lung lavage fluid following NO_2 and cigarette smoke exposure" Japanese Journal of Hygiene. 45. 363 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Manabu Hirai, Toshihiro Kawamoto, Yasushi Kodama et al.: "The inhibition of EGF binding by air pollutants" Japanese Journal of Industrial Health. 3i. 715 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahiro Yoshikawa;Keiichi Arashidani;Toshihiro Kawamoto;Yasushi Kodama: "Organ specificity of aryl hydrocarbon hydroxylase induction by cigarette smoke" Bulletin of Environmental Contamination and Toxicology. 44. 940-947 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 土屋 健三郎,児玉 泰 他: "地球環境問題から室内汚染までーリスクアセスメントー" 産業医科大学雑誌. 12. 457-461 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小山 倫浩,川本 俊弘,児玉 泰 他: "二酸化窒素およびタバコ煙曝露による肺胞洗浄液の変化" 日本衛生学雑誌. 45. 363 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 平井 学,川本 俊弘,児玉 泰 他: "大気汚染物質による上皮成長因子の細胞表面受容体への結合抑制について" 産業医学. 32. 715 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Masahiro Yoshikawa,Keiichi Arashidani,Toshihiro Kawamoto,Yasushi Kodama: "Organ specificity of aryl hydrocarbon hydroxylase induction by cigarette smoke" Bulletin of Environmental Contamnation and Toxicology. 44. 940-947 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 土屋 健三郎・児玉 泰 他: "地球環境問題から室内汚染までーリスクアセスメントー" 産業医科大学雑誌. 12. 457-461 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ed.by T.Yoshimura et al.: "Industrialization and Emetging Environmental Health IssuesーRisk Assessment and Risk Managementー" Princeton Scientific Pub.Co.,

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahiro Yoshikawa,Keiichi Arashidani,Toshihiro Kawamoto,Yasushi Kodama: "Organ specificity of aryl hydrocarbon hydroxylase induction by cigarette smoke" Bulletin of Environmental Contamnation and Toxicology. 44. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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