| Project/Area Number |
01570335
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | Saga Medical School |
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Principal Investigator |
FUJITANI Noboru Saga medical school, Department of Forensic Medicine, Research Associate, 医学部, 助手 (10156888)
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| Co-Investigator(Kenkyū-buntansha) |
SODESAKI Ken-ichiro Saga medical school, Department of Forensic Medicine, Research Associate, 医学部, 助手 (20216584)
YOSHIDA Ken-ichi Osaka University Medical School, Department of Legal Medicine, Research Associat, 医学部, 助手 (40166947)
MATOBA Ryoji Saga medical school, Department of Forensic Medicine, Professor, 医学部, 教授 (20107056)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Methamphetamine / Heart / Myosin isozyme / Actomyosin ATPase / Methampletamine / Myosinisoenzym / Methamphetamino |
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| Research Abstract |
We have reported that long-term administration of methamphetamine (MA) of which main pharmacologic reaction is releasing catecholamine from nerve endings induces rat cardiac lesions such as hypertrophy, myolysis, contraction band necrosis and disarray of myofibers. To further investigate the mechanism of MA induced cardiac injury we examined the myosin isozyme pattern and the actomyosin ATPase activity of the heart. The histological examination of r-at heart administered MA 1 mg/kg b. w. /day for 8 weeks and 12 weeks revealed hypertrophy, atrophy, myolysis and disarray of myofibers. Isozyme patterns of myosin were investigated by polyacrylamide gel electrophoresis in the presence of pyrophosphate as described by Hoh et al. and were estimated by densitometry. Though there was a significant difference between VI value of controls which were injected saline same volume for 8 w and 12 w (69.3 +- 8.0 % vs 56.2 +- 5.7 %, respectively), no significant difference was observed between controls and MA treated rat hearts in both groups. Mg^2-ATPase activity in the presence of 10 or 0.1muM Ca^<2+> were measured by previously reported method. In result, there were no significant differences in Mg^<2+>-ATPase activity at 10muM Ca^<2+> between 8 w and 12 w controls and each MA treated rats, but there was a significant increase of Mg^<2+>-ATPase activity at 0.1muM Ca^<2+> in 12 w HA treated rats compared with controls. The result suggest that there may be some changes in cardiac function after long-term MA administration as well as histologic changes.
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