| Project/Area Number |
01570345
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Legal medicine
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| Research Institution | University of Occupational and Environmental Health, Japan (1990)
Kanazawa Medical University (1989) |
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Principal Investigator |
TANAKA Noriyuki UOEH Professor, 医学部, 教授 (60126597)
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| Co-Investigator(Kenkyū-buntansha) |
KASAI Kentaro Kanazawa Instructor Medical university, 医学部, 助手 (40169397)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | ethanol degradation / ethanol production / contents of intestinal tract / intestinal bacteria / antibiotics / Toxycology / Ethanol decomposition / Ethanol metabolism / Intestinal microorganism |
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| Research Abstract |
In this study, the intestinal tract contents in rabbit were examined bacteriologically in vitro and in vivo to clarify the mechanism of ethanol metabolism in it.
1. In vivo study, it was demonstrated that ethanol was degraded and produced in the contents of cecum, colon and rectum.
2. Alcohol dehydrogenase participated in the ethanol degradation in cecum content. Catalase had little effect on the degradation.
3. These enzymes were created by enteric bacteria, and affected by chloramphenicol and colistin in vivo.
4. The ethanol degradation in cecum content accounted for 3.5% of all ethanol degradation in vivo.
5. In vitro study, enteric bacteria was demonstrated to degrade and produce the ethanol.
6. The ethanol degradation of enteric bacteria (E. Coli) in vitro was mediated by alcohol dehydrogenase and catalase.
The enteric bacteria that had a ability to degrade the ethanol was inhibited to multiply by chloramphenicol, streptomycin and colistin. It was suggested that the decrease of ethanol degradation by these antibiotics was the result of the inhibition to multiply the enteric bacteria.
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