| Project/Area Number |
01570380
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Faculty of Medicine, University of Tokyo |
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Principal Investigator |
TERANO Akira Faculty of Medicine, Univ. of Tokyo, Instructor Assistant Member, 医学部(病), 講師 (50155470)
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| Co-Investigator(Kenkyū-buntansha) |
MUTOH Hiroyuki Faculty of Medicine, Univ. of Tokyo, instructor Assistant, 医学部(病), 医員
OTA Shinichi Faculty of Medicine, Univ. of Tokyo, instructor Assistant Member, 医学部(病), 助手 (30185269)
中田 良 東京大学, 医学部, 助手
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Mucosal defense / Stomaca / Cell culture / prostaglandin / free rodical / Cytoprotection / 粘腫防御 / 細胞培養 / プロスタグランディ- / 胃粘膜 / viability / プロスタグラデイン / MTTアッセイ法 / 培養細胞 / 胃粘膜細胞保護作用 / 情報伝達機構 / グルタチオン |
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| Research Abstract |
The treatment of peptic ulcer disease has been changed by the recent use of H2 blocker and proton pump inhibitor. The recurrence rate of peptic ulcer. however, has been still high and of important problem from the medical as well as social view point. For the elucidation of this issue, the mechanisms of gastric mucosal protection would be crucial. With respect to the protective mechanisms of gastroduodenal mucosa, the concept of prostaglandin cytoprotection or the role of free radicals would be of great importance. The most important point of this issue so far, is whether this protection depends on microcirculation of gastric mucosa and whether or not prostaglandins or other cytoprotective agents truly protect gastric mucosal cells per se. In this investigation, we have clarified that gastric mucosal cells were truly protected in invitro model, using gastric monolayer culture system developed by us previously. Furthermore, the mechanisms of endogenous prostaglandin production have been revealed by employing this system from the standpoint of molecular biology.
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