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Molecular Biological Study on the Mechanism of Hepatocellular Injury of type B Hepatitis

Research Project

Project/Area Number 01570390
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

FUSAMOTO Hideyuki  Osaka University Medical School Lecturer, 医学部, 講師 (90124776)

Co-Investigator(Kenkyū-buntansha) SASAKI Yutaka  Osaka University Medical School Assistant, 医学部, 助手
KASAHARA Akinori  Osaka University Medical School Assistant, 医学部, 助手 (70214286)
HAYASHI Norio  Osaka University Medical School Lecturer, 医学部, 講師 (00144478)
片山 和宏  大阪大学, 医学部附属病院, 医員
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsType B Hepatitis / HBV / Molecular Biology
Research Abstract

In order to clarify the mechanism of hepatocellular injury in patients with type B hepatitis, we examined gamma-interferon production of peripheral blood mononuclear cells (PBMCs) from patients with type B hepatitis. Gamma-interferon production of PBMCs from patients with active liver injury were enhanced by HBcAg but not HBsAg, whereas those with inactive liver injury were not affected by HBcAg nor HBsAg. Further, These responsiveness of PBMCs to HBcAg closely correlated to serum levels of aminotransferase during their clinical course, but those to HBsAg did not at all. These results indicated that cellular immune response to HBcAg was responsible for hepatocellular injury in patients with type B hepatitis. Moreover, activated T cells in the liver had significantly increased compared with those in peripheral blood, and there were significantly more activated T cells in the livers of the patients with active liver disease than in those with inactive liver injury. HLA class I Ag was also expressed on the membrane of hapatocyte of patients with active liver injury. In contrast, we also showed that, in HBV-DNA integrated hepatoblastoma cell line, HBV replication suppressed the expression of HLA class I Ag on the membrane induced by gamma-interferon, and that pre-incubation of PBMCs with HBcAg/HBeAg suppressed gamma-interferon production of PHA-stimulated PBMCs. Our conclusion reached is that cellular immune response to HBcAg may cause liver injury and the poor responsiveness in patients with type B hepatitis can be responsible for establishment of chronicity of HBV infection.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 片山 和宏、他: "B型肝疾患における肝細胞障害機構の検討" 肝臓. 31. 1139-1142 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 竹原 徹郎、他: "肝組織浸潤リンパ球表面抗原のtwo color flew cytometric analysis" 肝臓. 31. 1038-1045 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takehara T.et al.: "TwoーDrmensional Flow Cytrometrio Analysis of Intrrhepstic Lymphocyte Substs from patients with Chronic Hepstitis" Digestive Disese and Science. 36. 87-91 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuki N.et al.: "Detection of Antibodies againet the Polynemese Gene Product in Hepatitis B Virus lnfection" Hipatclogy. 12. 193-198 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuki N.et al.: "Quantitstive analysis of preーS1 and preーS2 in relation to HBSAg expression" Hapatology. 11. 38-43 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 結城 暢一、他.: "B型肝炎ウィルス感染者におけるHBV関連DNAポリメラ-ゼ抗体の検討" 肝臓. 30. 1645-1651 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 春名 能通、他.: "B型慢性肝炎患者に対するインタ-フェロン療法における血中PreーS1抗原、PreーS2抗原の検討" 肝臓. 31. 5-11 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 三田 英治、他.: "慢性肝疫患におけるCーmyc および CーHaーras発現に関する検討" 肝臓. 31. 118-12 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takehara T, Hayashi N, Katayama K, Kasahara A, Fusamoto H, Kato M, Masuzawa M, Kamada T.: "Two-Dimensional Flow Cytometric Analysis of Intrahepatic Lymphocyte Subsets from Patients with Chronic Hepatitis." Dig Dis Sci. 36. 87-91 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuki N, Hayashi N, Kasahara A, Katayama K, Ueda K, Fusamoto H, Kamada T.: "Detection of Antibodies against the Polymerase Gene Product in Hepatitis B Virus Infection." Hepatology. 12. 193-198 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuki N, Hayashi N, Katayama K, Kasahara A, Ueda K, Fusamoto H, Sato N, Kamada T.: "Quantitative analysis of pre-S1 and pre-S2 in relation to HBsAg expression." Hepatology. 11. 38-43 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 片山 和宏,他: "B型肝疾患における肝細胞障害機構の検討" 肝臓. 31. 1139-1142 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹原 徹郎,他: "肝組織浸潤リンパ球表面抗原のtwoーcolor flow cytometric analysis" 肝臓. 31. 1038-1045 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takehara T,et al.: "TwoーDimensional Flow Cytometric Analysis of Intrahepctic Lymphocyte Subsets from Patients with Chromic Hepatitis" Digestive Disease and Science. 36. 87-91 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yuki N,et al.: "Detection of Antibodies against the Polymerase Gene Product in Hepatitis B Virus Infection" Hepatology. 12. 193-198 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yuki N,et al.: "Quantitatine analysis of preーS1 and preーS2 in relation to HBsAg expression." Hepatology. 11. 38-43 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 結城 暢一,他: "B型肝炎ウィルス感染者におけるHBV関連DNAポリメラ-ゼ抗体の検討" 肝臓. 30. 1645-1651 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 春名 能通,他: "B型慢性肝炎患者に対するインタ-フェロン療法における血中PreーS1抗原、PreーS2抗原の検討" 肝臓. 31. 5-11 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 三田 英治,他: "慢性肝疾患におけるcーmycおよびcーHaーras発現に関する検討" 肝臓. 31. 118-122 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹原徹郎,他.: "肝継織浸潤リンパ球表面抗原のtwoーcolor flow cytonetric analysis." 肝臓.

    • Related Report
      1989 Annual Research Report
  • [Publications] 片山和宏,他.: "B型肝疾患における肝細胞障害機構の検討" 肝臓.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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