• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic and Clinical Studies on the Mechanism of Liver Cell Necrosis in Hepatits Type B

Research Project

Project/Area Number 01570396
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionOkayama University

Principal Investigator

TSUJI Takao  Okayama University First Department of Internal Medicine Professor, 医学部, 教授 (80033306)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kazuhide  Okayama University First Department of Internal Medicine Assist Prof, 医学部附属病院, 助手 (90140491)
TOBE Kazuo  Okayama University Health Research Center Professor, 保健管理センター, 教授 (60135978)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHepatitis type B / Viral Hepatitis / Autoimmune Hepatitis / Liver Membrane Antigen / Anti-liver Membrane Antibody / Mechanism of Liver Cell Necrosis / Polymerized Human Serum Albumin / Adhesion Molecules / ICAMー1 / LFAー1 / 肝細胞レセプタ- / ウイルスレセプタ-
Research Abstract

(1)Purification and Analysis of Hepatocyte Receptor for Polymerized Human Serum Albumin : Supernatant of homogenate from the normal human liver was separated by SDS-PAGE and transferred to nitrocellulose membrane. The transferred proteins were reacted with polymerized human serum albumin(pHSA)labeled with horseradish peroxidase and two bands with molecular weight of 15 kD and 14.8 kD were detected. The two proteins were hepatocyte receptors for pHSA(hepatocyte-pAR). Immunofluorescence microscopy using antibody to hepatocyte-pAR revealed hepatocyte-pAR was localized in cytoplasm and cell membrane of normal human hepatocytes.
(2)Clinical Significance of Serum Hepatocyte-pAR and Antibody to Hepatocyte-pAR in Patients with Liver Diseases : Purified hepatocyte-pAR was coated on a microplate and serum antibody to hepatocyte-pAR was detected by ELISA. All sera from normal subjects were negative for antibody to hepatocyte-pAR. In patients with positive HBsAg. 56 % in HBeAg positive patients and … More 58 % in HBeAb-positive patients were positive for the antibody. Patients with positive HBsAb. especially with positive anti-liver membrane antibody, showed high frequency in hepatocyte-pAR antibody. Hepatocyte-pAR was detected in sera of some patients with positive HBeAg, suggesting the intimate relationship with liver cell injury.
(3)Fate of Polymerized Human Serum Albumin(pHSA)in vivo : pHSA was conjugated with colloidal gold and injected into the portal vein of rats. Human liver biopsy specimen was also perfused with colloidal gold conjugated with pHSA. Colloidal gold particles were taken up by sinusoidal endothelial cells and Kupffer cells through coated pits and vesicles. Further studies are needed to elucidate the interaction between pHSA and human hepatocytes.
(4)Significance of Adhesion Molecules in Liver Cell Necrosis : Adhesion molecules such as lymphocyte associate antigen-1(LFA-1)and intercellular adhesion molecules-1(ICAM-1)were examined in biopsy specimens from patients with chronic hepatitis type B. LFA-1 was expressed on lymphocytes infiltrating into the area such as piecemeal necrosis and focal necrosis. In such area, ICAM-1 was expressed on liver cell membrane in association with HLA class 1 antigen. These results suggest that adhesion molecule may play an important role in the process of liver cell necrosis. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 辻 孝夫他: "自己免疫性肝炎における肝細胞膜抗原(肝細胞ーpAR)とHCV抗体の意義に関する研究(1)" 難治性の肝炎調査研究班 平成元年度研究報告書. 37-39 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 辻 孝夫他: "自己免疫性肝炎における肝細胞膜抗体の意義に関する研究" 難治性の肝炎調査研究班 平成2年度研究報告書. 29-31 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩崎 良章他: "ELISA法によるB型肝炎ウイルスのpreーS(2)抗原定量化ーポリマ-化アルブミンレセプタ-(pAR)活性との検討" 消化器と免疫. 22. 150-154 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩崎 良章他: "B型肝炎ウイルスの血清preーS(1)抗原とpreーS(2)抗原の臨床的意義の検討" 消化器と免疫. 23. 173-177 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 辻 孝夫: "PreーS抗原(アルブミンレセプタ-)" 内科. 63. 1327 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 辻 孝夫他: "ウイルス肝炎と免疫機能" 病態生理. 10. 110-121 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshioka T,et al: "Frontiers of Mucosal Immunology Vol2" Tsuchiya M,Nagura H,Hibi T,Moro I, 660 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 辻 孝夫他: "最新内科学大系(48)ウイルス肝炎" 山村 雄一,吉利 和, 392 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji T, et al: "Significance of liver membrane antigen (hepatocyte-pAR) and anti-HCV in autoimmune hepatitis." Annual Report of Research Committee on Intractable Hepatitis. 1990. 37-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji T, et al: "Significance of liver membrane antibody in autoimmune hepatitis." Annual Report of Research Committee on Intractable Hepatitis. 1991. (29-31)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasaki Y, et al: "Quantitative assay of HBV-preS(2) antigen by ELISA-Its correlation with polymerized albumin receptor activity" Digestive Organ and Immunology. 22. 150-154 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasaki Y, et al: "Clinical significance of serum HBV-pre-S(1) and pre-S(2) antigens." Digestive Organ and Immunology. 23. 173-177 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji T: "Pre-S antigen (albumin receptor)." Medicine. 63. 1327 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasaki Y, et al: "Clinical significance of serum HBV-pre-S(1) and pre-S(2) antigens." Jpn J Clin Immun. 12. 259-263 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasaki Y, et al: "Clinical significance of serum hepatocyte-polymerized albumin receptor (hepatocyte-pAR) and antibody to hepatocyte-pAR." Digestive Organ and Immunology. 24. 182-186 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshioka T, et al.: "Fate of chemically modified albumin in liver sinusoidal cells." Progress in Hepatic Sinusoidal Cell Studies. 3. 88-92 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji T, et al.: "Viral hepatitis and immune function." Medicina Philosophica. 10. 110-121 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshioka T, et al.: "Receptor-mediated endocytosis of formaldehyde-treated albumin by rat and human liver sinusoidal cells." Frontiers of Mucosal Immunology. 2. 217-218 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsuji T, et al.: "Acute hepatitis type B." Internal Medicine. 119-137 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 吉岡 敏文,他: "肝類洞壁細胞における化学修降アルブミソの動態" 肝類洞壁細胞研究の進歩. 3. 88-91 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshio Ito,et al.: "Long term intermittent administration of interferon I'm patients with lromi nonーA,nonーB hepatitis" Gastroenterologin Japonica. 26. 187-193 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hajime Hada,et al: "Seguenes Variations in the Envelope Pratain of the Heprtitis C Virus:Comparison with Partial cDNA Segunce of a New Variant Virus Obtained by the Polymures Chain keaction" Acta Med Okayama. 45. 347-355 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 山田 剛太郎,辻 孝夫: "C型肝炎の病理像の特微" 内科. 67. 860-866 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshioka T et al: "Frontiers of mucosal imnunology" M.Tsuchiya et al, 660 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 辻 孝夫 他: "自己免疫性肝炎における肝細胞膜抗原(肝細胞ーpAR)の意義に関する研究" 平成元年度難治性の肝炎調査研究斑自己免疫性肝炎分科会研究報告書.

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩崎 良章 他: "血清中の肝細胞ポリマ-化アルブミンレセプタ-(肝細胞ーpAR)・肝細胞ーpAR抗体の臨床的意義" 消化器と免疫. 24. 73-77 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 辻 孝夫 他: "自己免疫性肝炎における肝細胞膜抗体の意義に関する研究" 平成2年度難治性肝炎調査研究斑自己免疫性肝炎分科会研究報告書.

    • Related Report
      1990 Annual Research Report
  • [Publications] Ito,T,et al: "Longーterm intermittent administration of interferonーα in patients with chronic nonーA,nonB hepatitis" Gastroenterolgia Japonica. 26. 1090-1096 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 辻孝夫 他: "自己免疫性肝炎における肝細胞膜抗原(肝細胞ーpAR)の意義に関する研究" 平成元年度 難治性の肝炎調査研究班 自己免疫性肝炎分科会 研究報告書.

    • Related Report
      1989 Annual Research Report
  • [Publications] 岩崎良章 他: "血清中の肝細胞ポリマ-化アルブミンレセプタ-(肝細胞ーpAR)・肝細胞ーpAR抗体の臨床的意義" 消化器と免疫. 24. 73-77 (1990)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi