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Preclinical Diagnosis of Hepatocellular Carcinoma by Lectin Affinity Electrophoresis

Research Project

Project/Area Number 01570401
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionHealth Research Center, Kagawa University

Principal Investigator

TAKETA Kazuhisa  Kagawa Univ. Health Res. Center Professor, 保健管理センター, 教授 (50033080)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordshepatocellular carcinoma alpha-fetoprotein / alpha-fetoprotein / lectin affinity electrophoresis / α-フェトプロテイン / レクチン / 親和電気泳動 / 抗体親和転写法 / 画像診断 / 慢性肝炎 / 肝硬変
Research Abstract

1. Alpha-Fetoprotein (AFP) was fractionated by lectin affinity electrophoresis with lentil Lectin (LCA-A) and erythroagglutinating Phytohemagglutinin (E-PHA) on added cases of chronic hepatits, cirrhosis and Hapatocellular Carcinama (HCC). AFP-L3 and/or AFP-P4 was positive (AFP-L3 > 15%, AFP-P4 > 12%) in all the 44 patients with HCC having serum AFP levels greater than 1,000 ng/ml, in 85% of 20 HCC cases having 1,000 ng/ml - 200 ng/ml, and in 88% of 16 HCC cases having AFP below 200 ng/ml.
2. Patients, including added cases, with chronic hepatitis and liver cirrhosis having increased serum levels of AFP without localized lesions of the liver were analyzed for lectin reactivities of AFP by affinity electrophoresis and antibody-affinity blotting. Among 51 patients without increased proportions of lentil lectin-reactive AFP-L3 and E-PHA-reactive AFP-P4, 4 patients had HCC during the follow-up period of 1 - 2 years. On the other hand, 5 patients with increased proportions of AFP-L3 and/or AFP-P4 all had HCC within 1 year and 10 months.
3. Cirrhotic patients having non-diagnostic space-occupying lesions of the liver with increased AFP-L3 and/or AFP-P4 were Ultimately diagnosed as having HCC.
4. The results indicated that AFP-L3 and AFP-P4 could serve as preclinical markers for diagnosis, or for defining a high-risk group, of patients with HCC before localization of HCC by imaging modalities.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 武田 和久: "肝細胞癌の発生と診断 5.画像診断以前の早期診断" 消化器癌. 1. 51-57 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuhisa Taketa: "Preclinical diagnosis of hepatocellular carcinoma before imaging" Shokakigan. 1-1. 57-57 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 武田 和久: "肝細胞癌の発生と診断 5.画像診断以前の早期診断" 消化器癌. 1. 51-57 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 武田和久、他: "AFPのレクチン反応性の変化に基づく肝細胞癌の高危険群の設定(第26回日本肝癌研究会発表)"

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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