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A Monoclonal Antibody Directed Against Activation Antigen on Alveolar Macrophages.

Research Project

Project/Area Number 01570427
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionSecond Division, Department of Internal Medicine, Hamamatsu University School of Medicine.

Principal Investigator

CHIDA Kingo  Hamamatsu University School of Medicine, Assistant Professor, 医学部, 助手 (40197611)

Co-Investigator(Kenkyū-buntansha) SATO Atsuhiko  Hamamatsu Univertity School of Medicine, Associate Professor, 医学部, 助教授 (60027109)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
KeywordsAlveolar Macrophage / Activated Macrophage / Anti Rabbit Antibody / Function Associated Antigen / Monoclonal Antibody / Phagocytosis / BCG / 抗肺胞マクロファ-ジ単クロ-ン性抗体 / 家兎肺胞マクロファ-ジ / 表面膜抗原
Research Abstract

We produced a monoclonal antibody against rabbit activated alveolar macrophage, in order to clarify the immunological roles of lung macrophages. BALB/c mice were immunized with bronchoalveolar lavage (BAL) cells obtained from a rabbit sensitized by heat-killed BCG intravenous injection. Spleen cells prepared from these mice were fused with the NS-1 plasmacytoma cell line using hybridoma technology, and we raised a monoclonal antibody called AM-1. The screening and characterization of this monoclonal antibody were carried out employing cellular radioimmunoassay, flow cytometric analysis, and immunohistochemical methods. AM-1 did not react with either blood monocytes or BAL-macrophages obtained from normal rabbits, but was positive for BAL-macrophages from BCG vaccinated rabbits. The percentage of positive cells for BAL-macrophages from rabbits at 7 days after vaccination was about 90%. The positive rate gradually decreased in relation to the time since BCG sensitization. The immunoglobulin subclass of AM-1 was IgG2b checked by Ouchterlony's method. Interestingly, incubation with either 10% fetal calf serum or macrophage activating factor caused increases in AM-1 reactivity of alveolar macrophages, and pretreatment of alveolar macrophages with AM-1 resulted in significantly reduced phagocytic capacity.
These results indicates that expression of surface anti AM-1 antigen is associated with an early activation process of alveolar macrophages, and also suggests that expression of anti AM-1 antigen appears to be involved in some of the macrophage functions.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 志知 泉,佐藤 篤彦,千田 金吾: "家兎の活性化肺胞マクロファ-ジを認識する単クロ-ン抗体,AMー1" 日本胸部疾患学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] I.Shichi,A.Sato,K.Chida: "A monoclonal antibody,AMー1;directed against activation antigen on rabbit alveolar mauophages" American Review Respiratory Disease.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] I. Shishi, A. Sato, K. Chida: "A Monoclonal Antibody, AM-1, Directed Against Activation Antigen in Rabbit Alveolar Macrophages" Journal, Volume-Number Pages Concerned, Year Am. Rev. Respir. Dis. 141(4). 647

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 志知 泉、佐藤 篤彦、千田 金吾: "家兎の活性化肺胞マクロファ-ジを認識する単クロ-ン抗体,AMー1" 日本胸部疾患学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] I.Shichi,A.Sato,K.Chida: "A monoclonal antibody,AMー1,directed against activation antigen on rabbit alveolar macrophages" American Review Respiratory Disease.

    • Related Report
      1990 Annual Research Report
  • [Publications] 志知泉,佐藤篤彦,千田金吾: "家兎の活性化肺胞マクロファ-ジを認識する単クロ-ン抗体、AM-1" 日本胸部疾患学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] I.SHICHI,A.Sato,R.Chida.: "A Monoclonal Antibody,AM-1,Ditrcted Against Activation antigen on Rabbit Alveolan Macrophages" American Review of Respiratory Disease.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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