| Project/Area Number |
01570438
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Tohoku University |
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Principal Investigator |
OHARA Yoshiro Department of Neurological Sciences Associate Professor, 医学部, 助教授 (50203914)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Tetsuro Fukushima Medical College Department of Neurology Assistant Professor, 講師 (20171978)
KONNO Hidehiko Department of Neurological Sciences Associate Professor, 医学部, 助教授 (10091688)
IWASAKI Yuzo Department of Neurological Sciences Associate Professor and Chairman, 医学部, 教授 (00142927)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Theiler's virus / demyelination / oligodendrocyte / infectious cDNA / primary culture / 感染症cDNA |
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| Research Abstract |
Theller's virus (TV) is divided into two subgroups. GDVII subgroups strains cause acute polloencephalomyelitis in the spinal cords of mice, whereas DA subgroup strains cause a persistent demyelinating disease in spite of the presence of high antibody titers against TV in the sera and CSF. As a possible mechanism to discriminate two subgroups with regard to the demyelinating activity, different cytotropism to oligodendrocytes (OL) has been proposed. The major goal of this project is to clarify this point by using recombinant virus from those subgroup strains and by using primary culture of OL.
AL first, we generated a full-length cDNA of DA strain from three overlapping clones which had been already reported (Virology 164 : 245-255, 1988). Virus derived from transfection of transcripts produced a demyelinating disease indistinguishable from that of wild-type virus. The infectious clone provides a critical reagent for the production of interstrain recombinant viruses to help indentify genetic loci responsible for the cytotropism in OL and demyelination as well.
On the other hand, two strains, i. e. DA and GDVII, were inoculated into the primary culture of OL derived from C3H mice and the infection and the propagation were studied. Double staining using anti-galactocerebroside and anti-TV antibodies revealed both strains infected OL. In addition, the kinetics of cell-free and cell-associated infectivity were not significantly different between two strains. These in vitro observation suggests that neither replication nor cell-associated infectivity is a sole factor in discriminating those two subgroup strains.
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