Basic and Clinical Study in the Pathogenesis and Treatment of Multiple Sclerosis

• Project/Area Number: 01570446
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Shinshu University
• Principal Investigator: SHOSEI Koh Shinshu University School of Medicine, Assistant Professor, 医学部, 講師 (80143981)
• Project Period (FY): 1989 – 1990
• Project Status: Completed (Fiscal Year 1990)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
• Keywords: Gangliosides / Experimental autoimmune encephalomyelitis (EAE) / Multiple sclerosis

Research Abstract

Gangliosides are known to suppress T-cell responses to mitogens and alloantigens. Gangliosides treatment (50mg/kg/rat subcutaneously every 12 hours) suppressed clinical signs of both actively induced and cell-transferred experimental allergic encephalomyelitis (EAE) in Lewis rats and guinea pig myelen basic protein (GPMBP) sensitized and cultured lymphocytes : mean score for gangliosides-treated rats 17.9, controls 32.4 in actively induced EAE ; gangliosides-treated rats 0, controls 1.4 in cell-transferred EAE. Lymph node cells or spleen cells from GPMBP-sensitized rats were stimulated in vitro with the specific sensitizing antigen, GPMBP, on with Con A. Purified bovine brain gangliosides significantly suppressed GPMBP-induced proliferation as a dose dependent manner. Gangliosides treatment during sensitization phase (from day-5 to day 3) did not suppress actively induced EAE. There were no significant differences in T-cell subsets of peripheral blood or spleen cells between gangliosides treated rats and controls by flow cytometry analysis. Taken together, these findings indicate that gangliosides primarily act on the immune effector phase of EAE.

Research Products

• [Publications] KOH, Shosei et al: "Fibrinolytic activities in the plasma and whole spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE)." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] KOH, Shosei: "Gangliosides suppress experimental autoimmune encephalomyelitis (EAE) by acting principally on immune effector phase of the disease." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] KOH, Shosei et al: "Expression of ICAMー1 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] S, Koh. et al.: "Fibrinolytic activities in the plasma and whole spinal cords of Lewis rats with experimental autoimmune encephalomyelitis (EAE)." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] S, Koh. et al.: "Gangliosides suppress experimental autoimmune encephalomyelitis (EAE) by acting principally on immune effector phase of the disease." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] S, Koh. et al.: "Expression of ICAM-1 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis." Journal of Neuroimmunology.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] KOH,Shosei et al: "Fibrinolytic activities in the plasma and whole spinal cords of Lewis rats with experimental autoimmune encephalomyelitis(EAE)." Journal of Neuroimmunology.
• [Publications] KOH,Shosei et al: "Gangliosides suppress experimental autoimmune encephalomyelitis(EAE) by acting principally on immune effector phase of disease." Journal of Neuroimmunology.
• [Publications] KOH,Shosei et al: "Expression of ICAMー1 in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis." Journal of Neuroimmunology.
• [Publications] KOH Shosei: "Gangliosides suppress experimental allergic encephalomyelitis(EAE)by acting principally on immune effector phase of disease." Journal of Neuroimmunology.