The establishment of the cell line which expresses both tyrosine hydroxylase and serotonin

• Project/Area Number: 01570454
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Jichi Medical School
• Principal Investigator: OGAWA Matsuo (1991) Jichi medical school Dept. Neurology, assistant, 医学部, 助手 (50194452); 新島 健司 (1989-1990) 自治医科大学, 医学部, 講師 (80150613)
• Co-Investigator(Kenkyū-buntansha): NIIJIMA Kenji Jichi Medical School Dept. Neurology, lecturer, 医学部, 講師 (80150613) ; 小川 松夫 自治医科大学, 医学部, 助手 (50194452)
• Project Period (FY): 1989 – 1991
• Project Status: Completed (Fiscal Year 1991)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: cell fusion method / tyrosine hydroxylase / serotonin / in situ hybridization / インサイチュハイブリダイゼ-ション / hybridization / cell line / 5ーhydroxytriptamine / serotonin / 5-hydroxytriptamine

Research Abstract

We established a cell line by the cell fusion method using polyethylene glycol. Results of chemical analysis showed that this cell line contained predominantly serotonin. Immunoreactivities for neuron-specific enolase, tyrosine hydroxylase and serotonin were observed in the cells of this fused cell line, but these cells were not stained by anti-glial fibrillary acidic protein antibody. From double immunostaining, serotonin immunoreactive cells of this cell line also showed TH-immunoreactivity. In in situ hybridization methods, mRNA of TH was expressed in the cells of this cell line. The morphologies of the cells in this cell line were of a variety of forms and staining intensities in immunostaining. We could not divide the homogeneous cells from this cell line. We assume that the morphological heterogeneity is characteristic of this cell line.

Research Products

• [Publications] Matsuo Ogawa et al.: "The establishment of the cell line which expresses both tyrosine hydroxylase and serotonin" Biogenic Amines.
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] M. Ogawa et al.: "The establishment of the cell line which expresses both tyrosine hydroxylase and serotonin" Biogenic Amines.
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] M.Ogawa et al.: "The establishment of the cell line which expresses both tyrosine hydroxylase and serotonin" Biogenic Amines.
• [Publications] Mieko Otsuka,et al.: "Marked Decrease Mitochondrial DNA With Multiple Deletions In a Patient With Familial Mitochondrial Myopathy." BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIOMS. 167(2). 680-685 (1990)
• [Publications] Masahiro Kumagai,et al.: "Interleukin 3 As a Trophic Factor for Central Cholinergic Neurons In Vitro and In Vivo." NEURON. 2. 429-436 (1990)
• [Publications] Kenji Niijima,et al.: "Enhanced Survival of Cultured Dopamine Neurons by Treatment With Solube Extracts From Chemically Deafferentiated Striatum of Aduit Rat Brain." BRAIN RESEARCH. 528. 151-154 (1990)
• [Publications] Niijima,K.,Araki,M.,Ogawa,M.,Nagatsu,I.,Sato,F.,Kimura,H.,and Yoshida,M.: "Enhanced survival of cultured dopamine neurons by soluble extracts from chemically deafferented striatum." Brain Research.
• [Publications] Niijima,K.,Araki,M.,Mizuno,Y.,Ogawa,M.,Nagatsu,I.,and Yoshida,M.: "N-methylisoquinolinium ion destroys mesencephalic dopamine neurons in culture." Neuroscience letter.
• [Publications] Niijima,K.,Araki,M.,Ogawa,M.,Nagatsu,I.,and Yoshida,M.: "Striatal extracts from patients with parkinson's disease promote neurite outgrowth from mesencephalic dopamine neurons." Neurology.
• [Publications] Fujimoto,K.,Yoshida,M.,Ikeguchi,K.,and Niijima,K.: "Imparement of active avoidance produced after destruction of pedunculopontine nucleus area in the rat." Neuroscience Research. 6. 321-328 (1989)
• [Publications] Kamegai,M.,Niijima,K.,Kunishita,T.,et al: "Interleukin 3 as a nerve trophic factor for central cholinergic neurons in vitro and in vivo." Neuron.(1990)
• [Publications] Ogawa,M.,Araki,M.,Nagatsu,I.,Nagatsu,T.,and Yoshida,M.: "Astroglial cell alterration caused by neurotoxins;immunohistochimical observations with antibodies to glial fibrillary acidic protein,laminin,and tyrosine hydroxylase" Experimental Neurology. 106. 187-196 (1989)