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Roles of Cytoskeletal Components for the Orientation and the Permeability of Cultured Endothelial Cells Exposed to Shear Stress

Research Project

Project/Area Number 01570464
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionUniversity of Tsukuba

Principal Investigator

SATO Masaaki  Univ. of Tsukuba, Inst. Basic Med. Sci., Associate Prof., 基礎医学系, 助教授 (30111371)

Co-Investigator(Kenkyū-buntansha) OHSHIMA Norio  Univ. of Tsukuba, Inst. Basic Med. Sci., Prof., 基礎医学系, 教授 (50015971)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCultured endothelial cell / Cytoskeleton / Shear stress / Extracellular matrix / Atherosclerosis / Mechanical property / Platelet thrombus / 内皮細胞 / 物質透過性 / マイクロフィラメント / 光-色素反応
Research Abstract

Porcine aortic endothelial cells (PAECs) were cultured and exposed to shear stress using the parallel plate flow chamber. The elongation and orientation of the cells were observed as compared to controls. This effect was highly dependent on such parameters as the level of shear stress and the duration of exposure. Staining of actin filament of endothelial cells showed changes in cytoskeletal structure prior to morphological changes of cell shape at an early stage of shear stress loading. After the flow experiment the mechanical properties were studied using the micropipette technique. The cells exposed to shear stress demonstrated a mechanical stiffness was significantly greater than that of control cells. The influence of shear stress on endothelial cell mechanical stiffness may be related to alteration in cytoskeletal structure. Using the same technique actin filaments were also shown to play important roles in viscoelastic behavior of the cells.
Effects of extracellular matrices (ECM … More s) on the cell shape and the cytoskeletal structure were analyzed on PAECs. The components of ECM used were type IV collagen, heparan sulfate, chondroitin sulfate and dermatan sulfate. The PAECs cultured on the mixed ECMs showed marked elongation and segmental orientation with randomly distributed cell axis even under a no-flow condition. The actin filaments were mostly observed in parallel with the cell axis. These findings suggest that ECM is one of the most important factors that acts together with shear stress to induce the variation of endothelial cell configuration in vivo.
Using the fluorescent dye-light technique platelet adhesion was found to be induced on PAECs and the platelet deposition grew on the light-irradiated area of the cells. In this in vitro thrombus model, a scanning electron microscopy showed that the platelets directly adhered to PAECs without the denudation.
Experiments on the permeability through PAECs are still continued while we have tried to remodel the apparatus. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 佐藤 正明: "ずり応力に対する培養内皮細胞の反応" 脈管学. 30. 1153-1156 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 正明: "血管内皮細胞とレオロジ-" 日本バイオレオロジ-学会誌. 4. 56-68 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato: "Mechanical properties of cultured endothelial cells exposed to shear stress" Frontiers Med. Biol. Engng.2(3). 171-175 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato: "Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties" ASME J. Biomech. Eng.112(3). 263-268 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Ookawa: "Changes in microstructure of cultured porcine aortic endothelial cells in the early stage after applying fluidーimposed shear stress" J. Biomechanics.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Ookawa: "MorPhological changes of cultured porcine aortic endothelial cells induced by extracellular matrices" Arteriosclerosis.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 正明(分担): "培風館" バイオエンジニアリングー計測と力学,生体への応用ー.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato et al.: "Morphological and functional changes of cultured endothelial cells in response to shear stress." J. Jap. Coll. Angiol.30(10). 1153-1156 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato et al.: "Vascular endothelial cells and rheology." J. Jap. Soc. Biorheol.4(2). 56-68 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato et al.: "Mechanical properties of cultured endothelial cells exposed to shear stress." Frontiers Med. Biol. Engng.2(3). 171-175 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. Sato et al.: "Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties." ASME J. Biomech. Eng.112(3). 263-268 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Ookawa et al.: "Changes in microstructure of cultured porcine aortic endothelial cells in the early stage after applying fluid-imposed shear stress." J. Biomechanics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Ookawa et al.: "Morphological changes of cultured porcine aortic endothelial cells induced by extracellular matrices." Arteriosclerosis.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M. sato et al.: "Bioengineering- Measurements and Mechanics, Its Application to Living Tissues-" Baifukan Press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 正明: "ずり応力に対する培養内皮細胞の反応" 脈管学. 30. 1153-1156 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 正明: "血管内皮細胞とレオロジ-" 日本バイオレオロジ-学会誌. 4. 56-68 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Sato: "Mechanical Properties of cultured endothelial cells exposed to shear stress." Frontiers Med.Biol.Engng.2(3). 171-175 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Sato: "Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties." ASME J.Biomech.Eng.112(3). 263-268 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Ookawa: "Changes in microstructure of cultured porcine aortic endothelial cells in the early stage after applying fluidーimposed shear stress." J.Biomechanics.

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Ookawa: "Morphological changes of cultured porcine aortic endothelial cells induced by extracellular matrices." Arteriosclerosis.

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤正明: "血管内皮細胞とレオロジ-" 日本バイオレオロジ-学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 佐藤正明: "ずり応力に対する培養内皮細胞の反応" 脈管学.

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Sato: "An in vitro platelet thrombus model produced on cultured endothelial cells" Experientia.

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Sato: "Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties" Journl of Biomechanical Engineering.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Ookawa: "Changes in microstructure of cultured endothelial cells in early stage after appling shear stress" Jounal of Cell Biology.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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