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Effect of sex hormone and IGF-I on skeletal qrowth and maturation--Activity of aromatase and 5-reductase in chondrocyte--

Research Project

Project/Area Number 01570519
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionYamanashi Medical College

Principal Investigator

OHYAMA Kenji  Yamanashi Med. College, Dept. of Pediatrics, 医学部, 講師 (80051861)

Co-Investigator(Kenkyū-buntansha) NAKAGOMI Yoshiko  Yamanashi Med. College, Dept. of Pediatrics Clinical Fellow, 医学部, 医員 (20198055)
FUJIMOTO Masatoshi  Yamanashi Med. College, Dept. of Pediatrics Clinical Fellow, 医学部, 助手 (20156912)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
KeywordsAromatase / 5-alpha-reductase / IGF-I / Chondrocyte / Androgen / Estrogen / 軟骨細胞 / 身長発育 / 骨成長 / 静止軟骨 / 5α-リダクタ-ゼ活性
Research Abstract

Using isolated rabbit chondrocytes, we investigated the effect of sex steroids produced by cartilarges on qrowth and maturation of bone. Both aromatase activity and 5-reductase activity existed in chondrocytes from ribs and articular cartilarges of 2-week-old Japanese white nibs. Chondrocytes from articular cartilarges had higher aromatase activity than those from rib cartilarges. In rib cartilarges, aromatase activity in growth plate was significantly higher than those in resting cartilarges. Preincubation of cultured chondrocytes with dexamethasone stimulated aromatase expression in all cell strains. The increment of dexamethasone-induced aromatase activity was significantly higher in chondrocytes from growth plate than in resting chondrocytes ( articular chondrocyte > rib growth chondrocyte > rib resting chondrocyte ), and was significantly higher in female chondrocyte than in male one. In contrast, 5-reductase activity was similar in each chondrocyte and in both sexes. Preincubation of chondrocytes with IGF-I did not induced activities of aromatase and 5-reductase.
These results suggest that chondrocytes can produce estrogen and androgen, and estrogen production increases, and the maturation of cartilarge and bone may be advanced by paracrine action of those steroids, especially estrogen action.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 大山 建司: "男児の血漿総SomatomedinーC濃度、血清Testosterone濃度の加齢変化に関する検討" 日本小児科学会雑誌. 93. 270-275 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 矢守 利次: "骨の成長、成熟に及ぼす性ホルモンの影響に関する研究" 日本小児科学会雑誌. 95. 47-54 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大山 建司: "成長の異常ー身長と骨年齢" 小児科臨床. 42. 789-797 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤本 昌敏: "Cyproterone acetateの培養外陰皮膚線維芽細胞aromatase活性に及ぼす影響に関する研究" 日本内分泌学会雑誌. 65. 585-602 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大山 建司: "小児科Mook(59):性の分化と成熟の異常" 金原出版(編集, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ooyama K, Fujimoto M, Nakagomi Y, Ohta M, et al: "Age-related changes in plasma somatomedin-c and serum testosterone concentrations in boys." The Journal of the Japan Pediatric Society. 93. 270-275 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fujimoto M: "Effect of cyproterone acetate on aromatase activity in cultured human genital skin fibroblasts." Folia Endoclinologica Japonica. 65. 585-602 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamori T: "Effect of sex steroid hormone on skeletal growth and maturation" The Journal of the Japan Pediatric Society. 95. 47-54 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大山 建司: "男児の血漿総SomatomedinーC濃度、血清Testo sterone濃度の加齢変化に関する検討" 日本小児科学会雑誌. 93. 270-275 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 矢守 利次: "骨の成長、成熟に及ぼす性ホルモンの影響に関する研究" 日本小児科学会雑誌. 95. 47-54 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大山 建司: "成長の異常ー身長と骨年齢" 小児科臨床. 42. 789-797 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤本 昌敏: "Cyproterone acetateの培養外陰部皮膚線維芽細胞aromatase活性に及ぼす影響に関する研究" 日本内分泌学会雑誌. 65. 585-602 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大山 建司: "小児科Mook(59):性の分化と成熟の異常" 金原出版(編集), (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 矢守利次,藤本昌敏,大山建司 他: "培養軟骨細胞におけるアロマタ-ゼ活性及び5α-レダクタ-ゼ活性について" 日本内分泌学会雑誌. 65. 886- (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 藤本昌敏,矢守利次,大山建司 他: "培養軟骨細胞における性ステロイドホルモン合成について" 第23回日本小児内分泌学会抄録集. 42- (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fujimoto,M.et al.: "Time-dependent Biphasic response of aromatase to Dexamethasone in cultured human skin fibroblasts." J.Clin.Endocrinol.Metab. 63. 468-474 (1986)

    • Related Report
      1989 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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