| Project/Area Number |
01570567
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Yamaguchi University School of Medicine |
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Principal Investigator |
YAMAMOTO Toshihiko Yamaguchi Univ. Sch. Med. Dept. Dermatology Research Assistant, 医学部, 助手 (40182638)
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| Co-Investigator(Kenkyū-buntansha) |
TAKATA Ichiro Yamaguchi Univ. Sch. Med. Dept. Dermatology Research Assistant, 医学部, 助手 (40216651)
NAKANO Junji Yamaguchi Univ. Hospital Dept. Dermatology Research Assistant, 医学部附属病院, 助手 (40198156)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | 表皮 / 糖脂質 / Epidermoside / glycolipid / esterocerebroside / O-acylglucosylceramide / linoleic acid / W-hydroxy fatlyacid |
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| Research Abstract |
Human epidermis has lipid-water barrier, the role of which owes much to seramides and glycolipids with linoleic acids. Acylglucosylceramide of glycolipids in human epidermis was different from that in pig epidermis. In order to study the biochemical function, we analyze the structure of acylglucesylceramide in human epidermis.
We extracted total lipids from human epidermis with chloroform-methanol and isolated glycolipids by DEAE Sephadex A-25 and Unisil column chromatography following high pertormance lipuid chromatography with a slica gel column. Structural determination of glycolipids was performed by means of TLC, GLC, GLC-mass spectrometry and ^1H-NMR.
As a result, human epidermal glycolipids contain acylglucosylceramides which consist of glucoses, linoleic acids, omega-hydroxy-fatty acids and long chain bases. Linoleic acids bound to omega-portion of omega-hydroxy-fatty acid with ester bounds and long chain bases are composed of sphingosines and phyto-sphingosines. Acylglucosylceramides containing phyto-sphingosines was first determined in epidermis.
Acylglucosylceramides having those structures are unique in epidermis, and we named them Epidermosides.
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