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Analysis of Oncogene and Tumor Suppressor Gene Mutations in Human Kin Tumors

Research Project

Project/Area Number 01570571
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

YASUNO Hirokazu  Kyoto Prefectural University of Medicine, Department of Dermatology, Professor, 医学部, 教授 (20079908)

Co-Investigator(Kenkyū-buntansha) YAMANISHI Kiyofumi  Kyoto Prefectural University of Medicine, Department of Dermatology, Research As, 医学部, 助手 (10182586)
池田 佳弘  京都府立大学, 医学部, 助手 (50202908)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSkin tumor / Oncogene / Ras gene / Point mutation / PCR / Tumor suppressor gene / p53 / ゲノム解析 / PCR
Research Abstract

The oncogenes and tumor suppressor genes have been suggested to be involved in tumorigenesis of human cell. In this project, on the basis of mechanisms of epidermal keratinocytes growth and its deregulation, we tried to detect the mutations of these genes in human skin tumors, especially derived from keratinocytes. The growth of keratinocytesis regulated by various growth control system including cAMP system. We first showed that the expression of Ha-ras oncogene introduced into cultured keratinocytes caused deregulation of the growth of the cells. Then we investigated the significance of the mutations of the Ha-ras gene in several kinds of human epidermal tumors. DNA from paraffin-embedded tissues of benign and malignant human epidermal tumors (27 samples from 25 patients ; seborrheic keratosis, keratoacanthoma, Bowen's disease, basal cell epithelioma, squamous cell carcinoma) were prepared and examined for point mutations of codons 12, 13, and 61 of Ha-ras gene by polymerase chain reaction and oligonucleotide hybridization. Only one sample of basal cell epithelioma and one sample of keratoacanthoma were found to carry an A to T transversion at the second position of codon 61. Th low incidence of Ha-ras mutations suggests that the mutational activation of the gene may not be primarily involved in human epidermal tumorigenesis. In addition, the research examining the tumor suppressor gene p53 mutations in 4 hot spots of mutation in various skin tumors is in progress.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Yamanishi,K.,et al.: "Cyclic AMP as a negative regulator of DNA synthesis in FRSK cells,a fetal rat epideral cell line." Journal of Dermatology. 16. 2-6 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamanishi,k.,et al.: "Growth advantage by overexpression of normal Harveyーras protoーoncogene in cultured rat epidermal keratinocytes." Archives of Dermatological Research. 282. 330-334 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Liew,FーM.,et al.: "Low incidence of Haーras oncogene mutations in human epidermal tumors." Cancer letter.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YAMANISHI, K., et al.: "Cyclic AMP as a negative regulator of DNA synthesis in FRSK cells, a fetal rat epidermal cell line." J. Dermatol.16. 2-6 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YAMANISHI, K., et al.: "Growth advantage by overexpression of normal Harvey-ras proto-oncogene in cultured rat epidermal keratinocytes." Arch. Dermatol. Res.282. 330-334 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Liew, F-M. et al.: "Low incidence of Ha-ras oncogene mutations in human epidermal tumors." Cancer Letter.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamanishi,K.,et al.: "Cyclic AMP as a negative regulator of DNA synthesis in FRSK cells,a fetal rat epideral cell line." Journal of Dermatology. 16. 2-6 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamanishi,k.,et al.: "Growth advantage by overexpression of normal Harveyーras protoーoncogene in cultured rat epidermal keratinocytes." Archives of Dermatological Research. 282. 330-334 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Liew,FーM.,et al.: "Low incidence of Haーras oncogene mutations in human epidermal tumors." Cancer letter.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kiyofumi Yamanishi: "Growrh advantage by ouerexpression of Ha-ras proto-oncoyene in cultured rat epidumal kerahinocytes." Archives of Dermatoloyical Peseach.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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