| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Research Abstract |
(1) Epidermal interleukin 1 in Contact Hypersensitivity (CHS) and UVB irradiation.
Using C3H/HeN mice, we studied the levels of epidermal IL-1 activity and mRNA for IL-1 alpha and beta of DNFB-induced CHS skin and UVB-burned skin. The lysates of the epidermis and dermis were used to estimate IL-1 activity, which was measured by C3H/HeJ mouse thymocyte proliferation assay. The mRNA levels for epidermal IL-1 alpha and beta were estimated by cytoplasmic dot hybridization. Both the IL-1 activity and the mRNA level for IL-1 alpha and beta were increased at 6 hrs after UVB irradiation. However, concerning CHS reaction at 6-24 hrs after challenge, the IL-1 activity decreased in epidermis but increased in dermis, although IL-1 mRNA levels in epidermis did not change. These data suggest that further production of epidermal IL-1 may not occur and stored epidermal IL-1 may be used during CHS reaction.
(2) The effects of interleukin 1 (IL-1) alpha and beta on cultured normal human epidermal keratinocytes (NHEK)
Although IL-1 alpha and beta are distinct gene products, they recognize the same receptor and share almost the same biological properties. To find out effects of both forms of IL-1 on proliferation and differentiation of keratinocytes, NHEK were cultured with IL-1 alpha, IL-1 beta or Ca2+. To estimate their proliferation, the number of cultured NHEK was counted and the 3H-thymidine uptake by NHEK was measured. To estimate their differentiation, epithelial keratins were studied by immunoblotting using two monoclonal anticytokeratin antibodies (PKK-1 and MA-904), a monoclonal antihuman filaggrin antibody, and rabbit antiserum against involucrin. The results of these experiments show that only IL-1 alpha, but not beta, suppresses the proliferation of NHEK and produces differentiation of them. These findings suggest that some biological properties may be different in IL-1 alpha and beta.
|
