Regulation of Type I Collagen Gene Expression in Fibrotic Skin Disorders
| Project/Area Number |
01570580
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
HATAMOCHI Atsushi Kawasaki Medical School, 医学部, 講師 (90172923)
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| Co-Investigator(Kenkyū-buntansha) |
OKA Daisuke Kawasaki Medical School, 医学部, 講師 (70148708)
UEKI Hiroaki Kawasaki Medical School, 医学部, 教授 (30069017)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Type I collagen / Gene expression / Transcription / Scleroderma / Werner's syndrome / Transformed / fibroblasts / Cutis laxa / 転写因子 / プロモ-タ- |
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| Research Abstract |
To a large extent the macromolecular organization and physiological function of connective tissue depends on the selection of the right collagen types. For the normal functioning of connective tissue an exact control of collagen gene expression is therefore required. Studies using cultured skin fibroblasts have already shown that fibroblasts from the involved skin from scleroderma and keloid patients show increased collagen synthesis as compared with those from normal controls. We studied collagen gene expression in dermal fibroblasts from patients with morphea and Werner's syndrome and transformed human fibroblasts. The level of m-RNA for type I collagen in fibroblasts derived from the inflammatory lesion were approximately 60% above those obtained from the normal looking lesion of the skin of a patient with morphea. Increased collagen synthesis accompanying elevated m-RNA levels incultured Werner's syndrome was observed. Decreased type I and type III collagen RNA transcription was observed in transformed human fibroblasts. We also studied collagen metabolism in cutis laxa fibroblasts. And we observed that increased collagenase gene expression associated with unaltered expression of type I and type III collagen.
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Report
(3 results)
Research Products
(15 results)
