| Project/Area Number |
01570581
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Tokyo Metropolitan Institute of Medical Science. |
|---|
Principal Investigator |
TANAKA Shinobu Tokyo Metropolitan Institute of Medical Science, Department of Laboratory Animal Science, research, 実験動物研究部門, 研究員 (50124475)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Takakazu Tokyo Metropolitan Institute of Medical Science, Department of Medical Chemistry, 医化学研究部門, 研究員 (60050514)
|
|---|
| Project Period (FY) |
1989 – 1990
|
| Project Status |
Completed (Fiscal Year 1990)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
|
|---|
| Keywords | Syphilis / Westernーblotting / IgG subclass |
|---|
| Research Abstract |
Changes in reactivities of Ig class and IgG subclass of anti-Treponema pallidum antibodies after treatment in sera from patients with clinical syphilis were assessed by sodium dodecyl sulfateーpolyacrylamide gel electrophoresis immunoblotting. A marked decrease in reactivity of IgM antibodies was noted after treatment and IgM antibody proved to be effective as a marker for evaluating the result of treatment. In occasional cases, however, IgM reactivity remained below the lower limit of sensitivity of the assay or failed to decline despite treatment. IgA antibodies decreased after treatment in limited cases of primary and secondary syphilis. No significant fall of IgG antibody was observed after treatment in the majority of syphilitic patients. Assessment made at the levels of IgG subclass antibodies demonstrated a decline in reactivity of one or more IgG subclass antibodies after treatment. The IgG subclasses and types of T. pallidum antigen(s) reacting with them that responded in conjunction with treatment, nevertheless, varied among patients. However, 56Kd antigen proved to be the most reliable marker antigen for the assessment of IgM reactivity. These results indicated that it is useful in evaluating the effect of treatment to monitor IgA and IgG subclass antibodies in those cases where IgM antibody fails to serve as a marker for the evaluation.
|
