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The roles of glutamic acid and gamma-aminobutyric acid in the convulsive mechanisms of El mice.

Research Project

Project/Area Number 01570607
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionOkayama University

Principal Investigator

YOKOI Isao  Okayama Univ. Med. Sch. Instructor, 医学部, 助手 (80150366)

Co-Investigator(Kenkyū-buntansha) HIRAMATSU Midori  Okayama Univ. Med. Sch. Instructor, 医学部, 助手 (70124790)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsExperimental epilepsy / El mice / Glutamate Release / Asparate Release / gamma-aminobutyrate Receptor / gamma-aminobutyrate Agonist / Glutamate Receptor / Glutamate Antagonist / E1マウス / アスパラギン酸 / グルタミン酸 / リリ-ス / 切片 / 大脳皮質 / 海馬 / 抗てんかん薬 / 放出 / 取り込み / オ-ト・レセプタ-
Research Abstract

El mice, which are inbred mutant and highly susceptible to convulsive seizures, have been considered a model of complex partial seizures with secondary generalization. In our study, we have observed(1)release of excitatory(aspartate(Asp)and Gglutamate(Glu))and inhibitory(gamma-aminobutyrate(GABA))neurotransmitters from cerebral cortical and hippocampal slices of El mice and(2)effects of Glu antagonists and GABA agonists on the occurrence of El mice convulsions in order to investigate the roles of amino acid neurotransmitters in the convulsive mechanisms of El mice.
Our experimental results are as follows.
1. Spontaneous release of Asp increased in the cortical slices.
2. Valproate, which inhibits the El mice convulsions, decreased spontaneous and K@AA+@BB -stimulated Asp release from cortical and hippocampal slices, but it had no effect on Glu or GABA release.
3. Baclofen, which is GABA_B receptor against, inhibit the occurrence of convulsions.
4. Some high affinity GABA_A receptor agonists inhibit the occurrence of convulsions.
5. ZAPA, which is a low affinity GABA_A receptor against, had no effect.
6. Glu receptors have been classified into N-methyl-D-aspartate(NMDA)type, kainate type and quisqualate type receptors. NMDA receptor antagonists and metabotropic NMDA receptor antagonist inhibit the occurrence of convulsions.
7. Kainate or quisqualate receptor antagonists had no effect.
8. These results suggest that GABA_A, NMDA and metabotropic Glu receptor may be involved in the occurrence mechanisms of the El mice convulsions.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Hideaki Kabuto: "Effects of muscimol and baclofen on levels of monoamines and th eir metabolites in the El mouse brain." Neurochem.Res.13. 1157-1161 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 陶山 ケイ子: "成長に伴うElマウス脳内モノアミンの変動。" Neurosciences. 17. 207-210 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Keiko Suyama: "Developmental changes of serotonin in El mouse brain with and without "tossing-up" stimulation." Neurosciences. 17. 317-323 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideaki Kabuto: "Effects of serotonin reuptake inhibitor on El mouse convulsion." Neurochem.Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N.A.Janjua: "Gamma-aminobutyric acid uptake is decreased in the hippocampus in a genetic modle of human temporal lobe epilepsy." Epilepsy Res.8. 71-74 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Midori Hiramatsu: "Increased aspartate release from brain slices of epilices of epileptic experimental animals and effect of valproate on it." Jpn.J.Psychiat.Neurol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Midori Hiramatsu: "Combined treatment of Shosai-ko-to-go-keshi-ka-shakuyaku-to (Tj-960) with low dose of diphenylacetate increased the latent time for pentylenetetrazol incduced convulsion and decreased brain lipid peroxidation in mice." Neurosciences.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akitane Mori: "International Perspective on Self-Regulation and Health." J.G.Carlson and A.R.Seifert (Eds.) Plenum Publishing Co.,New York, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideaki Kabuto: "Effects of muscimol and baclofen on levels of monoamines and their metabolites in the El mouse brain." Neurochem. Res.13. 1157-1161 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Keiko Suyama: "Developmental changes of serotonin in El mouse brain." Neurosciences. 17. 207-210 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Keiko Suyama: "Developmental changes serotonin in El mouse brain with and without "tossing-up" stimulation." Neurosciences. 17. 317-323 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] N. A. Janjua: "Gamma-aminobutyric acid uptake is decreased in the hippocampus in a genetic modle of human temporal lobe epilepsy." Epilepsy Res.8. 71-74 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Midori Hiramatsu: "Increased aspartate release from brain slices of epileptic experimental animals and effect of valproate on it." Jpn. J. Psychiat. Neurol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Midori Hiramatsu: "Combined treatment of Shosai-ko-to-go-keishi-ka-shakuyaku-to (Tj-960) with low dose of diphenylacetate increased the latent time for pentylenetetrazol induced convulsion and decreased brain lipid peroxidation in mice." Neurosciences.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideaki Kabuto: "Effects of serotonin reuptake inhibitor on El mouse convulsion." Neurochem. Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 陶山 ケイ子: "成長に伴うElマウス脳内モノアミンの変動" Neurosciences. 17. 207-210 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Keiko Suyama: "Developmental changes of serotonin in El mouse brain with and without “tossingーup"stimulation." Neurosciences. 17. 317-323 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hideaki Kabuto: "Effects of serotonin reuptake inhibitor on El mouse convulsion." Neurochem.Res.

    • Related Report
      1991 Annual Research Report
  • [Publications] J.G.Carlson and A.R.Seifert(Eds.),Akitane Mori: "International Perspective on SelfーRegulation and Health." Plenum Publishing Co.,New York, 11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Osaki,Y.,Hiramatsu,M.and Mori,A.: "Uptake and K^+ ーstimulated release of glutamate from cerebral cotrical and hippocampal slices in El mice,and dose effect of glutamate" NEUROSCIENCES. 16. 561-567 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiramatsu,M.,Edamatsu,R.,Kabuto,H.,Higashihara,Y.and Mori,A.: "Increased seizure susceptibility induced by guanidinoethane sulfonate in E1 mice and its relation to glutamatergic neurones" Neurochem.Res.14. 85-89 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Higashihara,Y.,Hiramatsu,M.and Mori,A.: "Aspartic acid release from cerebral cortical slices of El mice with high seizure susceptibility" Res.Comun.Chem.Pathol.Pharmacol.70. 155-171 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sato,K.,Morimoto,K.,Hiramatsu,M.,Mori,A.and Otsuki,S.: "Effect of a noncompetitive antagonist(MK801)of NMDA receptors on convulsions and brain amino acids level in El mice" Neurochem.Res.14. 741-744 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiramatsu,M.,Edamatsu,R.,Suzuki,S.,Shimada,M.and Mori,A.: "Regional excitatory and inhibitory amino acid levels in epileptic El mouse brain" Neurochem.Res.15. 821-825 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Janjua,N.A.,Mori,A.and Hiramatsu,M.: "Increased aspartic acid release from the ironーinduced epileptic focus" Epilepsy Res.6. 215-220 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mori,A.,Hiramatsu,M.,Kabuto,H.and Marescau,B: "Perspectives on Research in Emotional Stress Systems Research in Physiology Vo13" Gordon and Breach Science Publishers, 22 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mori,A.,Yokoi,I.,Kabuto,H.,Hiramatsu,M.,Kwon,M.J.,Shimada,M.and Akiyama,K.: "International Perspecctives on selfーregulation and health" Plenum Publishing Corporation, 11 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Isao Yokoi,Hideaki Kabuto,Kenji Akiyama,Akitane Mori,and Masayori Ozaki: "Tannins inhibit the occurrence of epileptic focus induced by FeCl_3 imjection in rats" The Japanese Journal of Psychiatry and Neurology. 43. 552-553 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideaki Kabuto,Isao Yokoi,Kiminao Mizukawa,and Akitane Mori: "Effects of an N-methyl-D-aspartate receptor agonist and antagonist CPP on the levels of dopamine and serotonin metabolites in rat striatum collected in vivo by using a brain dialysis technique" Neurochemical Research. 14. 1075-1080 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Midori Hiramatsu,Rei Edamatsu,Hideaki Kabuto,Yoshikazu Higashihara,and Akitane Mori: "Increased seizure susceptibility induced by guanidinoethane sulfonate in El mice and its relation to glutamatergic neurons" Neurochemical Research. 14. 85-90 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 平松緑,東原慶和,尾崎雄一郎,鈴木茂樹,大庭幸子,森昭胤: "Elマウス大脳皮質からのアスパラギン酸releaseとけいれんとの関係について" NEUROSCIENCES. 15. 118-120 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Akitane Mori,Midori Hiramatsu,Hideaki Kabuto and Bart Marescau: "Effect of emotional stress an convulsions and brain monoamines in El mice" perspective on Research in Emotional Stress Systems Reserch in Physiology. 13. 65-77 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Najma A.Janjua,Akitane Mori and Midori Hiramatsu: "Aspartic acid and glutamic acid release and uptake in the iron-induced model of post-traumatic epilepsy in the rat." NEUROSCIENCES. 15. 136-138 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiroshi Shiraga,Midori Hiramatsu and Akitane Mori: "GUANIDINES 2" Plenum Publishing Corporation, 10 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Midori Hiramatsu,Rei Edamatsu,Masahiro Kohno and Akitane Mori: "GUANIDINES 2" Plenum Publishing Corporation, 9 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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