| Project/Area Number |
01570647
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Nagasaki University School of medicine |
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Principal Investigator |
IZUMI Motonori Nagasaki University School of Medicine I'Depart. Med associate Prof, 医学部・第一内科, 助教授 (80039552)
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| Co-Investigator(Kenkyū-buntansha) |
NAGATAKI Shigeki Nagasaki University School of Medicine I'Depart. Med. assistant, 医学・第一内科, 助手 (00220062)
山下 俊一 長崎大学, 医学部, 助手 (30200679)
平湯 秀司 長崎大学, 医学部, 助手 (50165213)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | thyroid cancer / oncogene / PTC oncogene / retinoci acid / interleukin 1 / TSH receptor / thyroglobulin / thyroid peroxidase / レチノ-ル酸 / 分子生物学 |
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| Research Abstract |
Recently a new oncogene PTC has been reported to cause human papillary thyroid carcinoma, therefor we investigated whether this PTC gene exist in thyroid carcinoma using four thyroid cancer cell lines and 22 patients with papillary thyroid carcinoma. PTC gene was detected in only one thyroid carcinoma cell line and was not detect in other three cell lines and the whole 22 patients suggesting that PTC oncogene does not at least in Japan cause thyroid carcinoma.
Thyroid cell line NPA and WRO were investigated to find that ^<125>I TSH could find specfically these cell but TSH could not stimulate CAMP production and that cholexa toxin, forskolin and isoprotenol could stimuilate CAMP productor suggesting some abnormality in Gprotein coupled with TSH receptor. NPA could be stimulated to increase in the uptake of ^3H thymidine by CAMP or forskolin and this increase uptake is inhibited by IL_1 in dore dependent manner, suggesting that IL_1 has antitumorgenecity in some thyroid cancer.
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