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Effects of Extracellular Matrix on Adopose Conversion

Research Project

Project/Area Number 01570663
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionTokyo Metrop. Inst. of Med. Sci.

Principal Investigator

SATO Mayumi  Tokyo Metrop. Inst. of Med. Sci. Dept. of Tumor cell, 腫瘍細胞, 研究員 (50124459)

Co-Investigator(Kenkyū-buntansha) HIRAGUN Akiyoshi  Tokyo Metrop. Inst. of Med. Sci. Dept. of Tumor cell, 腫瘍細胞研究部門, 室長 (10109944)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsPreadipocyte / Differentiation / Fibronectin / Extracellular matrix / Serum free culture / Collagen / 無血清培養 / プロテア-ゼインヒビタ-
Research Abstract

A method is presented by which murine preadipose cells may be successfully grown and converted into mature adipocytes in the complete absence of serum. ST 13 preadipocyte were grown in a 1 : 1 mixture of RPMI 1640 and Ham F12 supplemented with sodium selenite, Hepes, transferrin, insulin, thrombin, and fetuin on various substrates, including four types of collagen, fibronectin, and laminin. A highly potent adipogenic agent, 3-hydroxyciglitazone (ADD), was essential for the induction of adipose conversion of ST 13 cells grown under serum-free conditions. As was true for cells in serum-supplemented medium, ADD treatment must begin in the logarithmic growth phase to induce efficient adipose conversion. Two major differences were observed, however, between serum-free and serum-supplemented adipose conversion systems. First, ST 13 cells grown without serum require a collagenous substrate (especially collagen type IV) for adipose conversion and for maintenance of a confluent monolayer, through not for cell proliferation. Second, insulin, which plays relatively minor role in the serum-supplemented system, is required absolutely in the serum-free system. The present observation demonstrates rhat ST 13 cells are tightly held in the preadipose state under serum-free conditions and appropriate adipogenic stimuli (such as ADD plus insulin) are essential for adipose conversion. The serum-free system will be useful for the identification and investigation of regulatory factors affecting adipose conversion.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Hiragun A: "Preadipocyte differentiation under serumーfree conditions:Essential requirements for collagenous substrate and adipogenic stimulus" in preparation.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawakami M: "Supression of lipoprotein lipase in 3T3ーL1 cells by a mediator produced by SEKI melanoma,a cachexiaーinducing human melanoma cell line." J.Biochem.109. 78-82 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato M: "Retinoic acid controls the adipogenic differentiation of ST 13 murine preadipose cell line." Int.J.of Obesity.14. 123 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato M.: "Demonstration of 1α25ーDihydroxyvitamin D_3 receptor in ST 13,and3T3 L1 preadipocytes and its inhibitory effect on preadipocyte differentiation." Int.J.of Obesity.14. 124 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiragun A: "Antidiabetic agent,ADDー4743,is potent inducer of adipose conversion in vitro." Int.J.of Obesity.14. 125 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oikawa,T.: "Inhibition of angiogenesis by vitamin D_3 analogues." Eur.J.Pharmacol.178. 247-250 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐藤 真友美: "細胞工学3章細胞培養技術" 丸善, 20 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 平郡 昭義: "暮らしと健康、がんとビタミン44巻" 保健同人社, 3 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiragun A. and Sato M.: "Preadipocyte Differentiation Under Serum-Free Conditions : Essential Requirements for Collagenous Substrate and Adipogenic Stimulus"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawakami M., Kondo Y., Imai Y., Hashiguchi M., Ogawa H., Hiragun A., Aotsuka S., Shibata S., Oda T., Murase T., and Takaku F.: "Supression of lipoprotein lipase in 3T3-L1 cells by a mediator produced by Seki melanoma, a cachexia-inducing human melanoma cell line." J. Biochem.109. 78-82 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato M., Hiragun A.: "Retinoic acid controls the adipogenic differentiation of ST 13 murine preadipose cell line." Int. J. of Obesity.,. vol 14. 123 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato M., and Hiragun A.,: "Demonstration of 1 alpha 25-Dihydroxyvitamin D3 receptor in ST 13, and 3T3 L1 preadipocytes and its inhibitory effect on preadipocyte differentiation." Int. J. Obesity.vol 14. 124 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiragun A. and Sato M.: "Antidiabetic agent, ADD-4743, is potent induce of adipose conversion in vitro." Int. J. of Obesity.vol 14. 125 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oikawa, T., Hirotani, K., Ogasawara, H. Katayama, K.: "Nakamura. H.,, and Iwaguti T., Hiragun. A. : Inhibition of angiogenesis by vitamin D_3 analoues." Eur. L. Pharmacol.178. 247-250 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sofue K , kanda k., Iwai M., Iida K., Yahara I., Hirai R.: "Hiragun A., Comparison of distribution of calspectin (nonerythroid spectrin or fodrin), alpha-actinin and vinculin between normal and avian sarcoma virus-transformed NRK cells." Exp. Cell Res.181. 256-262 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiragun A: "Preadipocyte differentiation under serumーfree conditions:Essential requirements for collagenous substrate and adipogenic stimulus" in preparation.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kawakami M: "Supression of lipoprotein lipase in 3T3ーL1 cells by a mediator produced by SEKI melanoma,a cachexiaーinducing human melanoma cell line." J.Biochem.109. 78-82 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sato M: "Retinoic acid controls the adipogenic differentiation of ST 13 murine preadipose cell line." Int.J.of Obesity.14. 123 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sato M: "Demonstration of 1α25ーDihydroxyvitamin D_3 receptor in ST 13,and 3T3 L1 preadipocytes and its inhibitory effect on preadipocyte" Int.J.of Obesity.14. 124 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiragun A: "Antidiabetic agent,ADDー4743,is potent inducer of adipose conversion in vitro." Int.J.of Obesity.14. 125 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Oikawa,T.: "Inhibition of angiogenesis by vitamin D_3 analogues." Eur.J.Pharmacol.178. 247-250 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 真友美: "細胞工学 3章 細胞培養技術" 丸善, 20 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 平郡 昭義: "暮らしと健康、がんとビタミン 44巻" 保健同人社, 3 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤真友美: "分化する脂肪細胞株" 化学と生物. 26. 590-593 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,M.,: "Functional studies of newly synthesized benzoic acid derivatives:Identification of highly potent retinoidーlike activity." J.Cell.Physiol.135. 179-188 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,M.: "Demonstration of 1α.25ーdihydroxy vitaminD_3 receptorーlike molecule in ST 13 and 3T3 L1 preadipocytes and its inhibitory effects on preadipocyte differentiation." J.Cell.Physiol.135. 545-550 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiragun,A.: "Preadipocyte differentiation in vitro:Identification of a highly active adipogenic agent" J.Cell.Physiol.134. 124-130 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,M.: "Differentiation associated increase of cAMPーdependent typeII protein kinase in a murine preadipose cell line(ST 13)." Biochem.Biophys.Acta.844. 296-305 (1985)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sato,M.: "Effects of synthetic analogs of tereocidine,indolactamーV,on the differentiation of murin preadipose cells." Cancer Letters,. 25. 261-269 (1985)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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