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Modification of the cell cycle of the dormant hemopoietic stem cells --Interleukin-6, G-CSF--

Research Project

Project/Area Number 01570694
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionInstitute of Medical Science, Tokyo University (1990)
Saitama Medical University (1989)

Principal Investigator

IKEBUCHI Kenji  Institute of Medical Science, Tokyo University Department of Cell Culture and Blood Transfusic Assistant Professor, 医科学研究所, 講師 (20175194)

Co-Investigator(Kenkyū-buntansha) TOHYAMA Hiroshi  Saitama Medical University Department of Laboratory Medicine Professor, 医学部, 教授 (00008278)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsHemopoietic stem cell / Cell cycle / Self-renewality / Interleukin-6 / Granulocyte colony-stimulating factor / Bone marrow stroma cell / インタ-ロイキン6 / 顆粒球コロニ-刺激因子 / 静止期(Go期) / 造血因子 / 老化
Research Abstract

Hemopoietic stem cells have been reported to stay in Go phase of the cell cycle and transfer to the active cell cycle at a random manner (so-called Stochastic theory). We found that human interleukin-6 (IL-6), granulocyte colony-stimulating factor (G-CSF) and IL-1 enhanced IL-3 dependent blast cell colony formation by shortening the Go poriod of the dormant stem cells.
Self-renewal capabilities of the hemopoietic stem cells are unique and necessary for keeping the stem cell population constant. In the presence of IL-3, probability for self-renewing was reported to be 0.589. Addition of IL-6, G-CSF or IL-1 did not change this probability. Addition of IL-4 or Leukemia inhibitory factor (LIF) decreased this probability to 0.52. Recently IL-11 and Stem cell factor (SCF) were reported to have the effects on the dormant stem cells, so we are planning to clieck their activities in the same manner.
There are controversies whether the dormant stem cells require any growth factors for their survival or not. Using the bone marrow cells from 5-FU-treated mice, we examined and suggested that IL-3 and G-CSF were necessary for their survival and the stem cells were dead in their absence.
We introduced the cinema method to analyze the cellular divisions and interactions. Following several divisions from a single immature hemopoietic cell showed that the paired daughter cells did not always divide at the same time and their cellular doubling times were variable. Stochastic manner in the proliferation process was directly shown. It is interesting to compare the leukemic cells with normal hemopoietic cells in their proliferative attitude, analyze the cell-cell interaction and cellular kinetic phenomena by using the cinema method.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 池淵 研二: "Interleukin-6の新しい造血因子活性" Progress in Medicine. 9. 1481-1495 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池淵 研二: "インターロイキン研究の現状(IL-1〜IL-7):IL-3" Biotherapy. 3. 1445-1451 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池淵 研二: "造血幹細胞の分化と造血因子" 代謝. 27. 535-536 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Itoh,Y.,Ikebuchi,K.,Asano,S.,Hirashima,K.: "IL-3 and G-CSF,as the survival factors for murine hemopoietic stem cells." (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi,K.,Itoh,Y.,Hirashima,.K,Asano,S.: "Observations of cellular proliferation process of the hemopoietic progenitor cells by the cinema method:The cellular doubling times are not always equal for the paired daugther cells." (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi,K.,Itoh,Y.,Hitashima,K.,Asano,S.: "The effects of synergistic factors on self-renewality of hemopoietic stem cells." (in preparation).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 三浦 恭定: "造血因子 研究の潮流" 中外医学社, 272 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高久 史麿: "Annual Review 血液 1991" 中外医学社, 253 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K.: "A new hemopoietic activity of interleukin-6" Progress in medicine. 9. 1481-1495 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K.: "Recent studies of interleukins (IL-1-IL-7) : IL-3" Biotherapy. 3. 1445-1451 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K.: "Hemopoietic activities of interleukin-3 in vitro, in vivo" Medical Immunology. 19. 493-500 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K.: "Differentiation of the hemopoietic stem cells and hemopoietic growth factors" Metabolism and disease. 27. 535-536 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Itoh, Y., Ikebuchi, K., Asano, S., Hirashima, K.: "IL-3 and G-CSF,as the survival factors for murine hemopoietic stem cells." (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K., Itoh, Y., Hirashima., K.Asano, S.: "Observations of cellular proliferation process of the hemopoietic progenitor cells by the cinema method : The cellular doubling times are not always equal for the paired daugther cells." (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ikebuchi, K., Itoh, Y., Hitashima.K., Asano, S.: "The effects of synergistic factors on self-renewality of hemopoietic stem cells." (in preparation).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 池淵 研二: "Interleukinー6の新しい造血因子活性" Progress in Medicne. 9. 1481-1495 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池淵 研二: "インタ-ロイキン研究の現状(ILー1〜ILー7):ILー3" Biotherapy. 3. 1445-1451 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池淵 研二: "ILー3 in vitro,in vivoの造血活性" Medical Immanology. 19. 493-500 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池淵 研二: "未分化造血幹細胞の増殖と分化" 免疫薬理. 8. 263-267 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池淵 研二: "造血幹細胞の分化と造血因子" 代謝. 27. 535-536 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 池淵 研二: "ILー11" 造血因子. 2. 68-74 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 三浦 恭定: "造血因子 研究の潮流" 中外医学社, 272 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高久 史麿: "Annual Review 血液 1991" 中外医学社, 253 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kenji Ikebuchi: "The effects of synergistic factors on self-renewality of hemopoietic stem cells."

    • Related Report
      1989 Annual Research Report
  • [Publications] Kenji Ikebuchi: "The modifications of characters of the hemopoietic stem cells by glucocorticoid."

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshihiro Itoh: "Modification of self-renewalities of murine hemopoietic stem cells by human leukemia inhibitory factor(LIF)."

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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