| Project/Area Number |
01570722
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Teikyo University, School of Medicine Trauma and Critical Care Center |
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Principal Investigator |
KOBAYASHI Kunio Teikyo University, School of Medicine, Professor, 医学部, 教授 (80082147)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Hiromasa Teikyo University, School of Medicine, Instructor, 医学部, 助手 (00187759)
HASEBE Masaharu Teikyo University, School of Medicine, Assistant Professor, 医学部, 講師 (00082264)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Burned Rat / Infection (Complication) / Protein and Energy Metabolism / Indirect Calorimetry / Energy Expenditure / LCT (Long-Chain Triglyceride) / MCT (Medium-Chain Triglyceride) / Ketone Bodies / 熱傷感染モデル / エネルギ-基質 / 間接熱量測定 / MCT / ICT |
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| Research Abstract |
Utilization of glucose (G), long-chain triglycerides (LCT) and medium-chain triglycerides (MCT) as energy sources were investigated in two different models : burned rat without infection (B-model) and with infection (BI-model). 20% burn was induced in male Sprague-Dawley rats weighing 320-340g. Normal saline was infused for the first 10 hours post-burn. And 10^9 CFU of Staphylococcus aureus was injected subcutaneously into the back of the animals in the BI-model. Both models were divided into 3 groups according to the TPN energy source (G,LCT or MCT), and were given isocaloric (156kcal//kg/day) and isonitrogenous (1gN/kg/day) TPN consisting one of the following : 100% of non-protein calories (NPC) as glucose (group G), 50% of NPC as glucose and 50% of NPC as LCT (group L), 50% of NPC as glucose and 50% of NPC as MCT (group M). After 12 hours of TPN, the animals were artificially ventilated and the energy expenditure (EE) was measured for the following 8 hours using indirect calorimetry
… More
. At the end of the EE measurement, blood, liver and muscle were harvested for measuring various metabolites.
EE in the B-model showed no differences among the three groups ; however, EE in group M(136<plus-minus>6kcal/kg/day) was significantly higher than that in group L(114<plus-minus>2) in the BI-model(p<0.01), indicating greater oxidation of MCT than LCT at the highly stressed condition of bacterial infection. Although was no difference in muscle protein content in the BI-model, in both models urinary nitrogen loss was significantly less in group M than in group G, suggesting a better nitrogen sparing effects of MCT with glucose than glucose alone. In the BI-model, serum total ketone bodies (TKB) were lower in group M(334<plus-minus>49 mumol/L) than in group L(612<plus-minus>96). This is contrast to the B-model where group M(866<plus-minus>82) had the highest TKB among the groups. This indicates an increased utilization of ketone bodies by peripheral tissues during infections complication. All the data suggest that MCT may be a highly oxidized and protein sparing energy source for burn injury specifically complicated with Less
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