| Project/Area Number |
01570725
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Tokyo Women's Medical College, Kianey Center |
|---|
Principal Investigator |
TERAOKA Satoshi Tokyo Women's Medical college, Assistant Professor, 医学部, 助教授 (20147383)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KANETSUNA Yukiko Tokyo Women's Medical College, Assistant Professor, 医学部, 助手 (40231261)
SHINKAI Masato Tokyo Women's Medical College, Assistant Professor, 医学部, 助手 (10216210)
FUJITA Shogo Tokyo Women's Medical College, Assistant Professor, 医学部, 助手 (70209055)
TOJINBRA Tamotsu Tokyo Women's Medical College, Assistant Professor, 医学部, 助手 (80197847)
FUCHINOUE Shouhei Tokyo Women's Medical College, Assistant Professor, 医学部, 講師 (10147382)
金綱 友木子 東京女子医科大学, 医学部, 助手
|
|---|
| Project Period (FY) |
1989 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Keywords | Hemodialysis / Brain metabolism / ^<31>P-MRS / 慢性〓不全 / 透析脳症 / 細胞内高エネルギ-リン酸化合物 / 細胞内pH / ATP / Magnetic Resonance Spectroscopy / 腎不全 |
|---|
| Research Abstract |
The effect of hemodialysis on intracellular pH in brain tissue was studied in ten uremic hares weighing about 2 kg. Uremia was induced by right nephrectomy and left subtotal nephrectomy. Dialysis was done with a small-sized hollow fiber unit and bicarbonate dialysate. The pH changes was monitored by use of 31 P nuclear magnetic resonance spectroscopy, using JEOL FX-200 NMR spectrometer (JOEL, Tokyo), operating at 80.75 MHz for 31 P-NMR. Each Spectrum was acquired by accumulation of 600 pulses with a pulse delay of 4.8 microsec. and acquisition time of about 0.5 seconds. Dialysis of 180 minutes with a blood flow rate between 200 ml/hr and 414 ml/hr was found to lower plasma urea and creatinine concentrations. However, no significant changes of the relative concentrations of the energy rich phosphates ATP, phosphomonoester, phosphodiester, inorganic phosphate and the pH were found in the front parietal brain. There was a possibility that the extent of uremia and the efficacy of dialysis in this experiment was insufficient to induce brain water and electrolyte metabolism alterations. MRS was a powerful new tool for studying effects of various methods of dialysis on brain pH and energy metabolism in vivo.
|
