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Experimental study on protection of pulmonary edema after lung and heart-lung transplantation

Research Project

Project/Area Number 01570787
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionNagasaki University

Principal Investigator

KAWAHARA Katsunobu  Nagasaki university School of Medicine Lecturer, 医学部, 講師 (80152990)

Co-Investigator(Kenkyū-buntansha) ITOYANAGI Noriake  Nagasaki university School of Medicine, 医学部, 医員
AKAMINE Sinji  Nagasaki university School of Medicine, 医学部, 医員
AYABE Hiroyoshi  Nagasaki university School of Medicine Assistant Professor, 医学部, 助教授 (60128147)
TOMITA Masao  Nagasaki university School of Medicine Professor, 医学部, 教授 (70039808)
本庄 誠司  長崎大学, 医学部, 医員
高橋 孝郎  長崎大学, 医学部, 医員
久野 博  長崎大学, 医学部, 医員
碇 秀樹  長崎大学, 医学部, 医員
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥700,000 (Direct Cost: ¥700,000)
KeywordsLung transplantation / Heart-lung transplantation / Oxygen radicals production / University of Wisconsin solution / Superoxide dismutase / alpha-tocopherol / Aserbic acid / glutathione / 心肺保存 / 心肺同時移植 / 単純浸漬冷却保存 / 肺水腫 / 肺血管外水分量 / 肺血管抵抗 / 組織過酸化脂質 / UWH / SOD / 還元型グルタチオン / CoQ10 / vitamine E
Research Abstract

Graft failure after lung or heart-lung transplantation is often caused by pulmonary edema resulting from ischemic reperfusion injury of the transplanted lung. It is well known that oxygen free radicals produced by neutrophils create reperfusion injury in iscliemic organs. We studied the prevelition of pulmonary reperfusion injury after canine lung and heart-lung allotransplantation.
1. To evaluate the efficacy of antioxidants such as glutathione, ascorbic acid (vitamin C), alphatocopherol (vitamin E), superoxide dismutase (SOD), and CoQ10, the left lung was flushed with Euro-Collins solution containing one of these antioxidants, and then a hilar clamp was performed in this lung. Ascorbic acid and alpha-tocopherol were administered intravenously prior to declamping. In the lungs treated with antioxidants, the production of oxygen free radicals decreased, and oxygenation was comparable to that in untreated lungs. Therefore, infusion of these antioxidants may be useful for preventing pulmo … More nary edema after transplantation.
2. Ischemic cold storage of canine left lungs at 4゚C for 24 hours was performed using a technique of flushing with preservative solution, and the lungs were reperfused with whole blood or leukocytedepleted blood. In the lungs flushed with Euro-Collins Solution, the lung water volume after reperfusion with leukocyte-depleted blood was lower than that with whole blood. On the other hand, in the lungs flushed with UW solution, which contained antioxidants such as glutathione and allopurinol, there was no difference between the lung water volume after reperfusion with whole vs leukocytedepleted blood. Results suggested that oxygen free radicals originating from neutrophils are one of the most important factors in the occurrence of ischemic reperfusion injury in grafted lungs, acid that perfusion with leukocyte-depleted blood prior to recirculation of the iscliemic lung is effective in preventing or alleviating pulmonary edema.
3. We successfully allotransplaned canine lungs after cold strage for 8 hours during which the lungs were flushed and preserved with Euro-Collins solution containing antioxidants such as glutathione, allopurinal, and SOD. Furthermore, allotransplantation of canine lungs flushed acid preserved with UW solution at 4゚C for 24 hours was also successful. However, the safety limit of the cold preservation time of a canine heart-lung block for transplantation was 4 hours even when using the UW solution for flushing and preserving the graft.
4. To evaluate the optimum potassium concentration in the UW solution, the lung water volume after reperfusion was evaluated using an isolated canine lung perfusion model. Canine left lungs were flushed with UW solution containing potassium at 120, 30, or 4 mEq/L, and preserved at 4゚C for 24 hours, then the lungs were reperfused with whole blood for 120 minutes. The lung water volume was lowest in the lungs flushed with UW solution containing potassium at 30 mEq/L, suggesting that this is the optimum potassium concentration in UW solution for preserving heart-lung grafts. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Katsunobu Kawahara: "Twenty-four-hour cauine lang pnservation using uw solution" Transplanfation. 51. 584-587 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsunobu Kawahara: "An experimental study of isolatcd double lung transplatition" Acta Medica Nagasahiensia. 36. 55-60 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Seiji Honjyo: "Ia expression and RNA content in BALF of allotrasplanted lungs in dogs" Acta Medica Nagasakiensia. 36. 153-161 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Hisano: "Experimental study on reperfusion injuryu to warm rschemic lunj-protective of CV-3611 and α-tocopherol on reperfusion lunj-" Acta Medica Nagasakieusia. 36. 243-251 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 川原 克信: "イヌにおける両肺分離移植" 今日の移植. 4. 631-635 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 川原 克信: "臓器移植の実際と臨床検査6.肺移植" 臨床病理. 時90. 67-81 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 谷口 英樹: "各種抗酸化剤の肺Reimplantation responseに対する効果ー特に組織学的見地ー" 第25回日本移植学会総会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 碇 秀樹: "イヌ摘出左肺潅流モデルによる保存液の評価" 第6回肺および心肺移植研究会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久野 博: "SOD,COQ10の阻血再潅流障害におよぼす抑制効果" 第6回肺および心肺移植研究会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 孝郎: "肺移植の再潅流障害に対するsuperoxide dismutase(SOD)の効果" 第8回Cytoprotection研究会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 孝郎: "肺移植の再潅流障害に対するsuperoxide dismutase(SOD),verapamilの効果" 第7回日本呼吸器外科学会総会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 孝郎: "肺移植の虚血再潅流障害に対するSODの効果" 文部省早田班プラグラム.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 谷口 英樹: "雑種成犬温阻血肺における各種抗酸化剤の効果に関する実験的検討" Cytoーprotection & biology. 7. 433-441 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Taniguchi: "The roles of GSH,SOD and solcoseryl in the prerention of ischemic Damage and reperfusion injury to narm ischemic lung-study of canine warm ischemic lung-" Acta Meclica Nagasakiensia. 35. 110-115 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Kawahara: "Graft function associated with oxygen free radicals immediately after transplantation" Acta Medica Nagasakinsia. 35. 225-227 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsunobu Kawahara: "Twenty-four hour canine lung preservation using UW solution" Transplantation. 51. 584-587 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsunobu Kawahara: "An experimental study of isolated double lung transplantation" Acta Medica Nagasakiensia. 36. 55-60 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Hisano: "Experimental study on reperfusion injury to warm ischemic lung -protective effect CV-3611 and alpha-tocopherol on reperfusion lung-" Acta Medica Nagasakiensia. 36. 243-251 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsunobu Kawahawa: "Graft function associated with oxygen free radicals immediately after transplantation" Acta Medica Nagasakiensia. 35. 225-227 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideki Taniguchi: "The roles of GSH, SOD, and solcoseryl in the prevention of ischemic damage and reperfusion injury to warm ischemic lung -study of canine warm ischemiclung-" Acta Medica Nagasakiensia. 35. 110-115 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsunobu Kawahara: "Twentyーfourーhour canine lung preservation using UW solution" Transplantation. 51. 584-587 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Katsunobu Kawahara: "An experimental study of isolated double lung transplantation" Acta Medica Nagasakiensia. 36. 55-60 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seiji Honjyou: "Ia expression and DNA content in BALF of allotransplantated lungs in dogs" Acta Medica Nagasakiensia. 36. 153-161 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiroshi Hisano: "Experimental study on reperfusion injyury to warm ischemic lung ーProtective effect of CVー3611 and αーtocopherol on reperfusion lungー" Acta Medica Nagasakiensia. 36. 243-251 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 川原 克信: "イヌにおける両肺分離移植" 今日の移植. 4. 631-635 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 川原 克信: "臓慢移植の実際と臨床検査 6.肺移植" 臨床病理. 特:90. 67-81 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久野 博,川原 克信,高橋 厚郎,碇 秀樹,本庄 誠司,村田 謙,綾部 公懿,富田 正雄: "肺移植の再潅流障害に対する.SOD CVー3611・αーfocopherolの効果" 日本外科学会雑誌. 臨時増刊号92. 104 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,村田 謙,綾部 公懿,富田 正雄: "Low potassium UW液による24時間肺保存の実験的検討" 日本呼吸器外科学会雑誌. 5. 324 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高橋 孝郎,川原 克信,久野 博,碇 秀樹,本庄 誠司,林田 謙,綾部 公懿,富田 正雄: "肺移植の再潅流傷害に対するSODの効果" 日本呼吸器外科学会雑誌. 5. 377 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,林田 謙,綾部 公懿,富田 正雄: "24時間保存肺の再潅流時の過酸化脂質の検討" 日本移植学会雑誌. 第27回総会臨時号26. 394 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高橋 孝郎,川原 克信,赤嶺 晋治,小林 誠博,林田 謙,糸柳 則昭,綾部 公懿,富田 正雄: "Cross circulation回路を用いたイヌ摘出肺機能評価モデル作成の試み" 第24回日本胸部外科学会九州地方会総会発表H3,7.18〜19.

    • Related Report
      1991 Annual Research Report
  • [Publications] 小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,林田 謙,綾部 公懿,富田 正雄: "Low K^+ UW液による24時間肺保存の実験的検討" 第24回日本胸部外科学会九州地方会総会発表H3.7.18〜19.

    • Related Report
      1991 Annual Research Report
  • [Publications] 高橋 孝郎,川原 克信,村岡 昌司,田川 務,中村 昭博,山本 聡,綾部 公懿,富田 正雄: "Cross circulationモデルによる保存肺の評価" 第8回肺および心肺移植研究会発表H4.1.25.

    • Related Report
      1991 Annual Research Report
  • [Publications] 田川 務,川原 克信,高橋 孝郎,中村 昭博,山本 聡,村岡 昌司,綾部 公懿,富田 正雄: "肺の阻血再潅流に対するPAF拮抗剤の効果" 第8回肺および心肺移植研究会発表H4.1.25.

    • Related Report
      1991 Annual Research Report
  • [Publications] 田川 務,川原 克信,高橋 孝郎,中村 昭博,山本 聡,村岡 昌司,綾部 公懿,富田 正雄: "長期保存肺における血小板活性化因子拮抗剤の効果" 第10回cytoprotection研究会発表H4.2.15.

    • Related Report
      1991 Annual Research Report
  • [Publications] 田川 務,川原 克信,高橋 孝郎,中村 昭博,山本 聡,村岡 昌司,綾部 公懿,富田 正雄: "保存肺における虚血再潅流障害に対するPAF拮抗剤の効果" 第19回臓器保存研究会(H4,3,24).

    • Related Report
      1991 Annual Research Report
  • [Publications] 田川 務,川原 克信,高橋 孝郎,中村 昭博,山本 聡,村岡 昌司,綾部 公懿,富田 正雄: "24時間保存肺における血小板活性化因子(PAF)拮抗剤の効果" 第9回日本呼吸外科学会(H4.5.21).

    • Related Report
      1991 Annual Research Report
  • [Publications] 村岡 昌司,川原 克信,高橋 孝郎,山本 聡,中村 昭博,田川 務,綾部 公懿,富田 正雄: "CMH solutionによる24時間冷却保存肺の機能に関する実験的検討" 第19回臓器保存研究会(H4.3.23).

    • Related Report
      1991 Annual Research Report
  • [Publications] 村岡 昌司,川原 克信,高橋 孝郎,山本 聡,中村 昭博,田川 務,綾部 公懿,富田 正雄: "m・CMH solutionによる24時間冷却保存肺の機能に関する実験的検討" 第9回日本呼吸器外科学会(H4.5.21).

    • Related Report
      1991 Annual Research Report
  • [Publications] 小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,林田 謙,綾部 公懿,富田 正雄: "肺保存におけるLow K^+ UW液の有用性" 第92回日本外科学会(H4,3.25).

    • Related Report
      1991 Annual Research Report
  • [Publications] 小林 誠博,川原 克信,高橋 孝郎,赤嶺 晋治,糸柳 則昭,林田 謙,綾部 公懿,富田 正雄: "Low potassium UW液による24時間肺保存の実験的検討" 第18回臓器保存研究会発表(H3,4.7).

    • Related Report
      1991 Annual Research Report
  • [Publications] Katsunobu Kawahara: "24ーhour canine lung preservation using UW solution" Transplantation. 51. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 碇 秀樹,: "Verapamilの阻血再潅流障害に対する効果(特に6時間冷却保存肺について)" 日本呼吸器外科学会雑誌. 4. 91 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 碇 秀樹: "24時間肺保存におけるUW液の評価" 日本胸部外科学会雑誌. 38. 415 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 碇 秀樹: "24時間保存肺の白血球除去血による潅流" 移植(日本移植学会雑誌). 25. 262 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久野 博: "肺移植の再潅流障害に対するSOD,CVー3611,αーtocopherolの効果" 移植(日本移植学会雑誌). 25. 407 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久野 博: "肺移植の再潅流障害に対するSOD,CVー3611,αーtocopherolの効果" 日本外科学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideki TANIGUCHI: "Experimental study on active oxygen species to warm ischemic lung--Availability of GSH,SOD,and alloprinol--" ACTA MEDICA NAGASAKIENSIA. 34. 177-187 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 谷口英樹,川原克信,富田正雄: "雑種成犬温阻血肺に対するGSH及びSODの効果についての実験的検討" 日本外科学会会誌. 89. 227

    • Related Report
      1989 Annual Research Report
  • [Publications] 高橋孝郎,川原克信,富田正雄: "肺移植の再灌流傷害に対するsuperoxide dismutase(SOD)verapamilの効果" 日本呼吸器外科学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 碇秀樹,川原克信,富田正雄: "Verapamilの阻血再灌流傷害に対する効果-特に6時間冷却保存肺について-" 日本呼吸器外科学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 谷口英樹,川原克信,富田正雄: "雑種成犬温阻血肺における各種抗酸化剤の効果に関する実験的検討" Cyto-protection & Biology. 7. 433-441 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 谷口英樹,川原克信,富田正雄: "各種抗酸化剤の肺Reimplantation responseに対する効果-特に組織学的見地-" 移植. 24. 167 (1984)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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