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Receptor Analysis of the Cholinergic and Dopaninergic Neuronal Graft in the Central Nervous System-in Vitro Macro-Autoradiographical Investigation

Research Project

Project/Area Number 01570814
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionOkayama University Medical School

Principal Investigator

MIZUKAWA Kiminao  Department of Anatomy, Lecturer, 医学部, 講師 (40137154)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordsneuronal transplantation / Dopamine receptor / muscarinic cholinergic receptor / receptor autoradiography / レセプタ- / レセプタ-・オ-トラジオグラフィ-法 / ムスカリニック・コリンレセプタ- / ドパミン・レセプタ-
Research Abstract

A. Cases of Dopaminergic graft :
Catecholamine producing cells (suspension of fetal mesencephalic implants cells) were transplanted in the ipsilateral striatum of rats with hemi-parkinsonism induced by injection of 6-OHDA on the left side of the substantia nigra. We investigated the alterations of the straital D1, D2 dopaminergic receptors and dopamine uptake sites of three groups of rats, i. e control, heim-parkinsonism rats. After grafting, straital D1 and D2dopaminergic receptors were normalized. There were very dense dopaminergic uptake sites in the control striatum by mazindol binding. On the ipsilateral lesion side of the straitum, dopamine uptake sites had disappeared. In the transplanted striatum, dopamine uptake sites were observed around of the graft.
B. Cases of cholinergic Graft :
The animal model for dementic state were made by the injection of ibotenic acid in the left Mynert nucleus of the rats. Small amount of cholinergic cells suspensions which were obtained from the basal ganglia of the fetus brains were transplanted in the ipsilateral cortex and/or striatum of the animal models. By using in vitro macro-autoradiography, it was shown that muscarinic cholinergic receptors were clearly induced in the grafted areas. In these studies, it is stressed that the modes of cholinergic receptor inductions in the grafted areas are different from those of the dopaminergic neurons.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Mizukawa,K.,H.Takayama,H.Sato,Z.Ota,K.Haba and N.Ogawa:"Alteration of muscarinic cholinergic receptors in the hippocampal formation of stressed rat:in vitro quantitative autoradiographic analysis" Brain Research. 478. 187-192 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa,K.,IーMing Tseng and N.Otsuka:"Quantitative electron microscopic analysis of postnatal development of zinc positive nerve endings in rat amygdala using Timm′s sulphatide silver technique" Developmental Brain Research. 50. 197-203 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa,K.,Y.Kimura and N.Otsuka:"Ultrastructure of tyrosine hydroxylase(TH)ーimmunoreactive cell bodies and nerve terminals in the rat midbrain periaqueductal central gray" Biochemical Research (Supplement1). 10. 269-276 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ogawa,N.,K.Mizukawa, K.Haba and H.Sato:"Neurotransmitter and receptor alterations in the rat persistent dyskinesia model induced by iminodipropionitricle" European Neurology (Supplement1). 30. 31-40 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa,K.,Y.H.Sora and N.Ogawa:"Ultrastructural changes of the substantia nigra,ventral tegmental area and striatum in 1ーmethylー4ーphenylー1,2,3,6ーtetrahydropyridine(MPTP)ーtreated mice" Res.Commu.Chem.Pathol.Pharmacol.,. 67. 307-320 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa,K.,K.Haba and N.Ogawa:"Alterations of protein kinase C in rat brain by intraventricular administration of thyrotropin releasing hormone(TRH):quantitative investigation by in vitro macroーautoradiography" Neurosciences. 16. 353-358 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 水川 公直、端谷 毅、西野 仁雄、小川 紀雄:"脳のレセプタ-と運動(永津俊治、吉田充男、金澤一郎、小川紀雄 編) 黒質細胞移植による運動機能の修復とド-パミン産生およびレセプタ-の変化" 平凡社, 211-224 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小川 紀雄、羽場 久美子、水川 公直:"脳のレセプタ-と運動(永津俊治、吉田充男、金澤一郎、小川紀雄 編) ジスキネジア・モデルにおける脳内神経伝達機構の異常" 平凡社, 225-239 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa, K., H. Takayama, H. Sato, Z. Oka, K. Haba and N. Ogawa: "Alteration of muscarinic cholinergic receptors in hippocampal formation of stressed rat : in vitro quantitative autoradiographic analysis." Brain Research. 478. 187-192 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa, K., I-Ming Tseng and N. Otsuka: "Quantitative electron microscopic analysis of postnatal development of zinc positive nerve endings in rat amygdala using Timm's sulphatide silver technique." Developmental Brain Research. 50. 197-203 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa, K., Y. Kimura and N. Otsuka: "Ultrastructure of tyrosine hydroxylase (TH)-immunoreactive cell bodies and nerve terminals in the rat midbrain periaqueductal central gray." Biochemical Research. 10 : (Supplement 1). 269-276 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ogawa, N., K. Mizukawa, K. Haba and H. Sato: "Neurotransmitter and receptor alterations in the rat persistent dyskinesia model induced by iminodipropionitricle." European Neurology. 30 : (Supplement 1). 31-40 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa, K., K. Haba and N. Ogawa: "Alterations of protein kinase C in rat brain by intraventricular administration of thyrotropin releasing hormone (TRH) : quantitative investigation by in vitro macro-autoradiography." Neurosciences. 16 :. 353-358

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa, K., Y. H. Sora and N. Ogawa: "Ultrastructural changes of the substantia nigra, ventral tegmental area and striatum in 1-menthyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-treated mice." Res. Commu. Chem. Pathol. Pharmacol.67(3). 307-320 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishino, H., Hashitani, T., Mizukawa, K., Ogawa, N. and Shiosaka, S.: "Long-term survival of grafted cells, dopamine synthesis/ release, receptor activity, and functional recovery after transplantation of fetal nigral cells in model animals of hemiparkinson's diesease." Alzheimer's and Parkinson's Diseases : Basic and Therapeutic Strategies, The Second International Conference on Alzheimer's and Parkinson's Diseases. Plenum Publishing Corporation. (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizukawa,K.et al.,: "Alterations of protein kinase C in rat brain by intraventricular administration of thyrotropin releasing hormone(TRH):quantitative investigation by in vitro macroーautoradiography" Neurosciences. 16. 353-358 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mizukawa,K.et al.,: "Ultrastructural changes of the substantia nigra,ventral tegmental area and striatum in 1ーmethylー4ー1,2,3,6ーtetrahydropyridine(MPTP)ーtreated mice" Res.Commu.Chem.Pathol.Pharmacol.,. 67. 307-320 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小川 紀雄 他: "老齢ラット脳におけるNMDAレセプタ-の減少とBifemelane Hydrocholoride投与による回復" Geriatric Medicine(老年医学). 28. 1845-1849 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐藤 博彦 他: "老齢ラット脳におけるソマトスタチン・レセプタ-の減少と塩酸b:femelane投与による増加作用ーレセプタ-・オ-トラジオグラフィ-法による検討ー" 日本老年医学会雑誌. 28. 23 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 水川 公直、他: "[脳と運動のレセプタ-] 黒質細胞移植による運動機能の修復とド-パミン産生およびレセプタ-の変化" 平凡社, (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishino,H.,et al.,: "[Alzheimer's and Parkinson's Disease II:Basic and Therapeutic Strategies] Longーterm survival of grafted cells,dopamine synthesis/release,receptor activity,and functional recovery after grafting of fetal cells in model animals of hemiーParkinson's disease" Plenum Publishing Corporation,

    • Related Report
      1990 Annual Research Report
  • [Publications] Mizukawa K.,et al.,: "Quantitative electron microscopic analysis of postnatal development of zinc positive nerve endings in rat amygdala using Timm's sulphatide silver technique" Developmental Brain Research. 50. 197-203 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogawa N.,et al.,: "Neurotransmitter and receptor alterations in the rat persistent dyskinesia model induced by iminodipropionitricle" European Neurology(Supplement). 30. 31-40 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Mizukawa K.,et al.,: "Aletrations of protein kinase C in rat brain by intraventricular administration of thyrotropin releasing hormone(TRH):quantitative investigation by in vitro macro-autoradiography" Neurosciences.

    • Related Report
      1989 Annual Research Report
  • [Publications] 水川公直 他: "『脳と運動のレセプタ-』黒質細胞移植による運動機能の修復とド-パミン産生およびレセプタ-の変化" 平凡社,

    • Related Report
      1989 Annual Research Report
  • [Publications] Nishino H.,et al.,: "Long-term Survival of Grafted Cells,Dopamine Synthesis/Release,Receptor Activity,and Functional Recovery after Grafting of Fetal Nigral Cells in Model Animals of Hemi-Parkinson's Disease「Alzheimer's and Parkinson's Disease II:Basic and Therapeutic Strategies」in press" Plenum Publishing Corporation,

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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