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Cell-Biological Study of Degenerative Structures (Rosenthal Fibers) in Gliomas.

Research Project

Project/Area Number 01570825
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionTokyo Metropolitan Institute of Gerontology

Principal Investigator

KOBAYASHI Shu  Tokyo Metropolitan Institute of Gerontology, 生理老化学研究系・神経生理部門, 研究員 (30161988)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Shu  Division of Physiological Aging Research, (30161988)
池内 聡  東京都老人総合研究所, 生理学部臨床第二研究室, 研究員
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsRosenthal fiber / Glioma / Degeneration / Ubiquitin / GFAP / Crystallin
Research Abstract

The aim of this project was to evaluate the degenerative process occuring in the cytoplasms of low-grade gliomas, such as Rosenthal fibers (RFs), and to clarify the biological characteristics of gliomas. Pertinent findings are described as below ; (1) Immunohistochemically we investigated RFs on specimen surgically removed from patients with qliomas (three cerebellar astrocytomas, three optic gliomas, two spinal cord astrocytomas, one spinal ganglioglioma).
We utilized sections from the formalin-fixed paraffin-embedded tissues and stained them with anti-GFAP antibody (Ab) and two anti-ubiquitin Abs... anti-PHF (paired helical filament) monoclonal Ab (DF2) which recognizes ubiquitin and anti-ubiquitin polyconal Ab provided by Dr. Haas. RFs were eosinophilic (brightred on H&E), purply-stained with PTAH (metachromasia), black with Heidemhein's iron-hematoxylin, and negative with PAS. The peripheral parts of RFs were intensely stained with anti-GFAP. DF2 showed intense staining on the ring … More around negative central core of most RFs in every specimen. Anti-ubiquitin polyclonal Ab gave the similar results as DF2. From the study on the serial sections, the peripheral halo of RFs was positive with both anti-GFAP and anti-ubiquitin Abs.
Both GFAP and ubiquitin were considered to exist in the periphery of RFs and they might play an important role to produce the amorphous core RFs. (2) We recognized granular bodies (Gb) in the cystoplasm of the glioma tissues among several cases described above, which are also considered to be degenerative change. Whole parts of Gbs were dark-blue on PTAH and positively stained with both GFAP and ubiquitin (a different finding from RFs). (3) We also showed the positive staining with GFAP and ubiguitin in the peripheral parts of RFs which appeared on the cyst wall of cerebellar hemangioblastoma in an autopsied case with massive cerebral hemorrhage. Recently, T. Iwaki reported that alphaB crystallin is the major constituent of RFs from the brain of Alexander disease. In progress is the investigation to evaluate what kind of role alphaB crystallin is playing in the glia and glioma cells by making a specific anti-alphaB crystallin antibody. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 小林 秀,他: "神経膠腫におけるRosenthal fiberの免疫組織化学的研究" 脳と神経. 42. 59-64 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 秀,他: "高齢者の頭部外傷に対する救急手術" 医学のあゆみ. 151. 371-373 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 和田 博史,他: "頸椎症、腰椎すべて卵を伴った下部脊髄の硬膜内髄外腫瘍と診断された高齢者の一症例" 臨床神経. 30. 869-872 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 福井 信介,他: "高齢者悪性リンパ腫と痴呆" Geriatric Neurosurgery. 3. 109-113 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shu Kobayashi,et al: "Rosenthal fibers in the cyst wall of a heming'oblastoma ー case report" Neusologiiaーmedico chirurgica.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小林 秀,他: "神経膠腫におけるgrauular bodyの免疫組織化学的検討"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shu Kobayashi, et al: "Immunohistochemical study on Rosenthal fibers in gliomas using anti-GFAP and anti-ubiquitin antibodies." Brain Nerve (Tokyo). 42. 59-64 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shu Kobayashi, et al: "Rosenthal fibers in the cyst wall of a hemangioblastoma, case report" Neurologica-medico Chirurgica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shu Kobayashi, et al: "Immunohistochemical study on granular bodies in gliomas using anti-GFAP and anti-ubiquitin antibodies"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 和田 博夫、他: "頸椎症、腰椎すべり症を伴った下部骨髄の硬膜内髄外腫瘍と診断された高齢者の一症例" 臨床神経. 30. 869-872 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 福井 信介、他: "高齢者悪性リンパ腫と痴呆" Geriatric Neurosurgery. 3. 109-113 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shu Kobayashi、et al: "Rosenthal fibers in the cyst wall of a hemangioblastonaーcase report" Neurologicaーmedico chirurgica^^ー.

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 秀、他: "神経膠腫におけるgranular bodyの免疫組織化学的検討"

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林秀: "神経膠腫におけるRosenthal fiberの免疫組織化学的研究" 脳と神経. 42. 59-64 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shu Kobayashi: "Rosenthal fiber in a case with cerebellar hemangioblastoma" Neurologica medicoーchirurgica submitted.

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林秀: "神経膠腫におけるgranulated bodyの免疫組織化学的検討" 投稿予定.

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林秀: "高齢者の頭部外傷に対する救急手術" 医学のあゆみ. 151. 371-373 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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