| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
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| Research Abstract |
The purpose of this study was to investigate whether adhesive polyphenolic protein (ppp) which was extracted from the marine mussels and some phenol derivatives were effective or not in protecting collagen fibers against proteolytic enzymes.
We purchased methyldopa, (+)-catechin, hesperitin, rutin, tannic acid, geranin which were examples of phenol derivatives with different molecular weights. Albumin and fibronectin were employed as controls. Azocoll, which is an insoluble azodye-bound collagen was mixed with various concentrations of these chemicals in dimethyl sulfoxide and acetic acid, respectively. The reaction was terminated by filtration and the product was washed several times to remove any excess of these chemicals. Azocoll specimens coated with the chemicals were lyophilized and suspended in tris buffer, and then the coloring reaction was started by adding the protease. The color yield obtained was measured at 520nm.
No significant protease digestion occurred when the azocoll was treated with ppp and tannic acid, and gradual decrease in the digestion of azocoll was observed after exposure to increasing concentration of geranin. In contrast, azocoll treated with the other phenol derivatives was completely digested by the protease.
An electron macroscopic view showed that the surface of the azocoll was entirely covered by ppp. On the other hand, azocoll treated with tannic acid and geranin has a rather similar surface to that of the controls.
From these results, there is a good possibility the ppp or some phenol compounds could be used to coat soft tissue, and could effect
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