| Project/Area Number |
01570883
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Urology
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
KOSHIDA Kiyoshi (1990-1991) Kanazawa University Urology Lecturer, 医学部附属病院, 講師 (70186667)
西野 昭夫 (1989) 金沢大学, 医学部附属病院(泌尿器科学), 助手 (80198481)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRANO Kazuyuki Gitu pharmaceutical University Professor, 薬剤学, 教授 (90057365)
UCHIBAYASHI Tadao Kanazawa University Urology Lecturer, 医学部附属病院, 講師 (90151894)
HISAZUMI Haruo Kanazawa University Urology Professor, 医学部, 教授 (70019537)
越田 潔 金沢大学, 医学部附属病院(泌尿器科学), 助手 (70186667)
|
|---|
| Project Period (FY) |
1989 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | Testicular tumor / placental-like / Tumor marker / Immunolocalization / セミノ-マ / 胎盤性アルカリフォスファタ-ゼ / 睾丸腫瘍 |
|---|
| Research Abstract |
The role of PLAP-like as a tumor marker was evaluated in 62 cases of testicular germ cell tumors. Positive rates in different histology as well as stages were as follows ; seminoma, stage I : 42%, stages II-III, 90%, nonseminoma, stage I : 17%, stages II-III : 44%. Serum PLAP-like values in stages II-III were significantly higher than those in stage I. Tissue PLAP-like concentrations in 32 seminomas and 15 nonseminomas were also investigated. Significant differences in PLAP-like concentrations were disclosed between seminoma tissues and nonseminoma tissues as well as between the cases with elevated serum levels and the cases with normal serum levels. Serum values in stage I turned to normal within a week after orchiectomy. Half-life in the circulation was calculated at 2 days. Four out of 93 cases(4.3%), 17 of 594 samples(2.9%)in a follow-up study were regarded as false positive. Thus PLAP-like appears to be a useful marker for seminoma.
To investigate a potential use of an anti-PLAP monoclonal antibody for detecting tumors, HPMS-1 MAb labeled with I-125 was injected to athymic mice bearing tumors producing PLAP. Although accumulation rate(%dose/g tissue)was gradually decreased in the tumor, clearance of the MAb in other organs were more rapidly. Thereby an increase of tumor/organ ratio with time was observed, resulted in a clear tumor imaging after 9 days of injection. PLAP concentrations in the transplanted tumors were lower than those of primary seminomas or metastatic lymphnodes of seminoma. Therefore a potential use of the anti-PLAP MAb(HPMS-1)for detecting metastatic lesion of seminoma could be expected.
|
