| Project/Area Number |
01570889
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Kyoto University |
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Principal Investigator |
KAKEHI Yoshiyuki (1990) Kyoto University, 1. Department Urolgy , Instructor 2. Faculty of Medicine, 医学部, 助手 (20214273)
西尾 恭規 (1989) 京都大学, 医学部, 講師 (50180584)
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| Co-Investigator(Kenkyū-buntansha) |
HORII Yasuki Kyoto University, 1. Department of Urolgy , Instructor 2. Faculty of Medicine, 医学部, 助手 (80211548)
筧 善行 京都大学, 医学部, 講師 (20214273)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Urinary bladder carcinogenesis / Rat / Inhibitor of Arachidonic acid / Anti-tumor-promoting / NDGA |
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| Research Abstract |
Previously, we reported that colony formation in soft agar was shown with rat bladder cells prior to papilloma development in rat treated BBN. And we provided a short-term screening method for detecting new carcinogens, promoters and antiーtumor-promoters. We also evaluated NDG A inhibited saccharin-induced colony formation, suggesting that it has anti-tumor-promoting activity in rat bladder carcinogenesis. We studied long-term experiment on the anti-tumor-promoting effect of NDGA on urinary bladder carcinogenesis in rats induced by BBN. Six-wk-old male Fischer 344 rats were distributed among 8 groups. The were given drinking with (groups 5-8) or without (groups 1-4) 0.05% BBN for 4weeks, and then for 36weeks, they were given powdered diet containing 5% Sodium Saccharin (groups 2and 6). All surviving animals were killed in week 40. Histological abnormalities were investigated. The incidence and numbers of precarcinomatous lesions were significantly lower in group 6 (87%) than 7 (55%). Group 1, 2, 3, and 4 did not show any abnormalities of the bladder epithelium. These results confirm that NDGA have anti-tumor-promoting effects on bladder carcinogenesis in rats.
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