| Project/Area Number |
01570937
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Oita Medical University |
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Principal Investigator |
MIYAKAWA Isao Dept. of Obstet. & Gynecol., Oita Medical University, Professor., 医学部, 教授 (70040607)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Hiroyuki Dept. of Obstet. & Gynecol., Oita Medical University, Instructor., 医学部, 助手 (50210050)
SADANAGA Akemi Dept. of Obstet. & Gynecol., Oita Medical University, Instructor., 医学部, 助手 (00196205)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | steroid sulfatase / estrogen metabolism / fete-placental function / ichthyosis |
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| Research Abstract |
Materials
Blood samples were collected from 7 patients with RXLI(age ; 2 - 22 yr.), 6 carriers of RXLI(32- 48 yr.), and 12 nonpregnant women(22 - 38 yr.), 30 pregnant women(24- 36 yr. ; 1st trimester n=9, 3rd trimester n=21), 15 fetuses(cord blood), 18 male adults(26- 46 yr.), 5 male children(2- 10 yr.)as controls.
Results
Steroid sulfatase activity was extremely lower in patients with RXLI of below 0.005 and 0.079 <plus-minus> 0.018(mean <plus-minus> SD)p mol/mg protein/8 h. in carriers of RXLI. Also, activities in control groups were 0.108 + 0.028 in nonpregnant women, 0.104 <plus-minus> 0.029 in the 1st trimester 0.139 <plus-minus> 0.029 in 3rd trimester of pregnancy, 0.116 <plus-minus> 0.024 in fetuses(umbilical cord blood), 0.088 <plus-minus> 0.014 in male adults and 0.107 <plus-minus> 0.019 p mol/mg protein/8 h. in male children,
Steroid sulfatase activity was significantly lower(p<0.001)in patients with RXLI than other groups and significantly greater in the 3rd trimester pregnant women of 0.139 <plus-minus> 0.029 than nonpregnant women of 0.108 <plus-minus> 0.028(P<0.01)and males of 0.088 <plus-minus> 0.014(P<O. 001). Also, sulfatase activity in the 3rd trimester pregnant women was higher(P<0.02)that of fe
We found only a small difference(p<0.05)in sulfatase activity in carriers of RXLI and normal females with considerable overtaping between the two groups.
Those results suggest that our assay method of steroid sulfatase activity in leucocytes offers a fast and safe method for the diagnosis of placental steroid sulfatase deficiency and the identification of patient with X-linked ichthyosis.
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