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Isolation and characterization of an novel oncodevelopmentalprotein from human malignant ascitic fluid.

Research Project

Project/Area Number 01570939
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKitasato University School of Medicine

Principal Investigator

NAKAI Mitsuo  Kitasato University School of Medicin, Dept. of OB/GYN., Professor, 医学部, 教授 (50050399)

Co-Investigator(Kenkyū-buntansha) IINO Joji  北里大学, 医学部, 助手 (30176052)
ISHIKAWA Masakazu  北里大学, 医学部, 助手 (40184494)
YAMAURA Noboru  北里大学, 看護学部, 助教授 (60050621)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsTumor-associated protein / Oncedevelopmental protein / Pregnancy-associated protein / Tumor marker / Isolation / Characterization / Serum concentration / Gynecological malignant diseases / 腹水抗原 / 卵巣癌 / 精製 / 特性解析
Research Abstract

A tumor-associated antigen that shares antigenicity with a pregnancy-associated protein has been detected in ascitic fluid of patients with advanced ovarian can cer. The protein was prepared from malignant ascitic fluid by the combination of poly (ethylene glycol) 4000 fractionation, DEAE-Sepharose column chromatography and prepardtive polyacrylamide gel electrophresis (PAGE). Antiserum against an malignant ascitic antigen (MASA) was raised in rabbits. The rabbit antiserum was applied to a column of normal human plasma (NHP)-coupled Sepharose, and specific antibodies against MASA (anti-MAsA) was highly purified. Subsequently, by the use of anti-MAsA-coupled Sepharose, highly purified MASA was obtained. The preparation thus obtained was analyzed on sodium dodecyl sulfate (SDS)-PAGE, Using this technique, a single antigenically active component was identified" The moleculermass of this MASA species was calculated to be 27 kDa by SDS-PAGE.
Highly sensitive assay method employed ELISA was established by the use of the monospecific anti-MAsA. With this methody- the smallest detectable amount detectable in a sample was 0.00125 U/ml. The sensitivity 6f the ELISA is 1, 000-fold higher than that of a previous electroimmunoassay employed a modified Laurell's method. With this method, it will be possible to establish the normal level range, which is a prerequisite for precise evaluation of the increase in abnormal conditions. In conclusion, the present study shows that estimation of the MASA will be an usefulladfunct for the management of patients with gynecological malignant diseases.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] NAKAI M,ATSUMI M,SONE Y and ARAI M: "Quantitative estimation of an alpha 2 pregnancy-asociated protein in normal preqnancy by means of an electroimmunoassay." Clinica Chimica Acta. 182. 103-109 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 石川 雄一,剣持 稔,黒須 不二男,中居 光生: "悪性腹水における新しい腫瘍関連蛋白の精製と性質について" 第10回腫瘍マ-カ-研究会記録. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 馬嶋 恒雄 中居 光生: "新しい妊娠関連蛋白の単純放射免疫拡散による測定法の考案と妊娠時動態" 日本不妊学会雑誌. 36. 101-106 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] NAKAI M,ISHIKAWA M and KAWAUCHI H: "Identification of an ectopic developmental antigen that appears in malignant asctitic fluid." European Journal of Obstetrics & Gynecology and Reproductive Biology. 42. 61-69 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] NAKAI M,KAWAUCHI H,ATSUMI M,MAJIMA,T and ISHIKAWA M: "A novel tumor-associated protein:clinical significance of serum levels in various clinical condutions with special reference to gynecological malignant diseases." European Journal of Obsterics & Gynecology and Reproductive Biology. 43. 143-147 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中居 光生: "新しい「がん発生蛋白」の特性解析とその臨床知見" 病態生理.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 馬嶋 恒雄: "新しい「がん発生蛋白」の血中濃度測定の臨床的意義ー産婦人科諸種病態とくに悪性における動態ー" 日本産科婦人科学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakai M: "Quantitative estimation of an alpha 2 pregnancy-associated protein in normal pregnancy by means of an electroimmunoassay." Clin Chim Acta. 182. 103-109 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishikawa M: "Purification and characterization of an novel tumor-associated protein in malignanat ascitic fluid." Proceedings of the Xth Annual Meeting of the Tumor Marker.226-228 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Majima T: "Quantitation of a new pregnancy-associated protein in pregnancy sera by means of a single redial immunodiffusion technique." Jpn J Fertil Steril. 36. 444-451 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakai M: "Identification of an ectopic developmental antigen that appears in malignant ascitic fluid." Eur J Obstet Gynecol Reprod Biol. 42. 61-69 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kami K: "Immunocytochemical investigation of an ectopic developmental antigen (a_2-PAP) in human placentae." Acta Anat Nippon. 66. 290 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakai M: "A Novel tumor-associated protein : clinical significance of serum levels in various clinical conditions with special reference to gynecological malignant diseases." Eur J Obstet Gynecol ReproduBiol. 43. 143-147 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakai M: "Characterization and clinical application of an novel oncodevelopmental protein" Medicine phylosophica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kami K: "Cell-biological study of a novel tumor-associated ascitic antigen." Acta Histochem Cytochem. 24. 518 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuo Nakair,Masakazu Ishikawa and Hirohito Kawauchi: "Identification of an ectopic developmental antigen that appears in malignant ascitic fluid" European Journal of Obstetrics & Gynecology and Reproductive Biology. 42. 61-69 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mitsuo Nakai,Hirohito Kawauchi,Masanori Atsumi,Tsuneo Majima and Masakazu Ishikawa: "A Novel tumorーassociated protein:clinical significance of serum levels in various clinical conditions with special reference to gynecological malignant diトeases" European Journal of Obstetrics & Gynecology and Reproductive Biology. 43. 143-147 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 馬嶋 恒雄,中居 光生: "新しい妊娠関連蛋白の単純放射免疫拡散による測定法の考案と妊娠時動態" 日本不妊学会雑誌. 36. 444-451 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 中居 光生: "新しい「がん発生蛋白」の特性解析とその臨床知見" 病態生理. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 馬嶋 恒雄: "新しい「がん発生蛋白」の血中濃度測定の臨床的意義ー産婦人科諸種病態,とくに悪性疾患における動態ー" 日本産科婦人科学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 馬嶋 恒雄: "新しい妊娠関連蛋白の単純放射免疫拡散法による測定法の考案と妊娠時動態" 日本不妊学会雑誌. 36. 110-117 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 馬嶋 恒雄: "新しい「がん発生蛋白」の血中濃度測定の臨床的意義ー産婦人科諸痙病態、とくに悪性疾患における動態ー" 日本産科婦人科学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAI,MITSUO: "Identification of an ectopic developmental antigen that appears in in malignant ascitic fluid" European Journal of Obstetrics and Gynecology,and Reproductive Biology(under revision).

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAI,MITSUO: "A novel tumorーassociated protein: clinical significance of serum levels in various clinical conditions with special reference to gynecological malignant diseases" European Journal of Obstetrics and Gynecology,and Reproductive Biology.

    • Related Report
      1990 Annual Research Report
  • [Publications] NAKAI,MITSUO: "Quantitative estimation of a_2 pregnancy-associated protein in normal pregnancy by electroimmunoassay" Clinica Chimica Acta. 182. 103-108 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] NAKAI,MITSUO: "A novel tumor-associated protein in malignant ascitic fluid:purification and characterization" International Journal of Cancer(submitted for publication).

    • Related Report
      1989 Annual Research Report
  • [Publications] 馬嶋恒雄: "新しい妊娠関連蛋白の単純放射免疫拡散による測定法の考案と妊娠時動態" 日本産科婦人科学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 馬嶋恒雄: "新しい「ガン発生蛋白」の血中濃度測定の臨床的意義-産婦人科諸種病態とくに悪性疾患における動態-" 日本産科婦人科学会誌.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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