• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of culturing retinal ganglion cells and myelination

Research Project

Project/Area Number 01570983
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

TAKAHASHI Hiroshi  UOEH, Dept Ophthalmol., Assistant Professor, 医学部, 講師 (00129511)

Co-Investigator(Kenkyū-buntansha) MIYATA Hiroshi  Keio Univ., Dept Ophthalmol., Instructor, 医学部, 助手 (60174190)
FURUKAWA Hiroshi  UOEH, Dept Anatomy, Assistant Professor, 医学部, 講師 (80131936)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsRetina / Ganglion cell / Oligodendrocyte / Myelin / Tissue culture
Research Abstract

Establishment of pure method of culturing retinal ganglion cells is very important for basic science and clinic because of revealing pathogenesis of demyelination, multiple sclerosis and developing transplantation of retinal neurons, especially ganglion cells.
Enzyme digestion-Percoll density gradient, panning and filter paper methods were used for culturing pure retinal ganglion cells. We revealed these methods had some merits and some demerits. In the density grandient method, cells were separated three fractions, I to III. Large, middle and small cells were cultured, respectively. Large cells might be ganglion cells, and then they were surrounded with flat cells or Muller cells.
Therefore, ganglion cells in fraction I were mixed with other cells.
The panning method was very expensive and cells were characteristically pure, but the cell number was very low. After using filter paper method, purity of gangion cells was excellent, but the numbers were very low.
Myelination was studied in co-culturing retinal explant and oligodendrocytes. These cells were twined around retinal neurites, and were attacted with neurites. These results suggest that neurites might be interacted with oligodendrocytes. However, silver staining was negative and myelin protein was not found.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 高橋 広: "培養網膜神経節細胞突起に対する低浸透圧の影響" あたらしい眼科. 8. 409-412 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiroshi Takahashi, Shinobu Akiya, Hidenori Horie and Tesuya Ogata: "Responses of Cultured Retinal Neurites to Hypotonic Environment" J. Eye (Atarashii Ganka). 409-412 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高橋 広: "培養網膜神経節細胞突起に対する低浸透圧の影響" あたらしい眼科. 8. 409-412 (1991)

    • Related Report
      1991 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi