| Project/Area Number |
01570997
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Tokyo dental college |
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Principal Investigator |
KATO Tetsuo Tokyo Dental College, Department of Microbiology, Research Assistant, 歯学部・微生物 助手 (00159253)
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| Co-Investigator(Kenkyū-buntansha) |
NAITO Yuko Tokyo Dental College, Department of Microbiology, Assistant Professor, 歯学部・微生物, 講師 (00147258)
OKUDA Katsuji Tokyo Dental College, Department of Microbiology, Professor, 歯学部・微生物, 教授 (40085741)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Periodontopathic Bacteria / Lipopolysaccharide / Lipid A / Mitogenic Activity / Polyclonal B-cell Activation / Monoclonal Antibody / Antiーidiotype Antibody / 歯周病 / Fusobacterium nucleatum / マイトジェン活性 |
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| Research Abstract |
The antigenic structure of lipopolysaccharides (LPS) of Fusobacterium nucleatum and Eikenella corrodens was analyzed with prepared murine monoclonal antibodies. Three monoclonal antibodies (isotype IgM) against F. nucleatum LPS were crossーreactive with LPS from E. corrodens. Two out of 3 monoclonal antibodies (isotype IgM) produced by immunization with E. corrodens LPS crossーreacted with LPS from F. nucleatum. Western immunoblotting analysis demonstrated that the 5 antibodies reacted with the common core epitope of LPSs from F. mucleatum and E. corrodens. Another monoclonal antibody (isotype IgG3) against E. corrodens LPS reacted with the O-side chain epitope of E. corrodens LPS and crossーreacted with the core epitopes of LPSs from Fusobacterium necrophorum and Capnocytophaga ochracea. Four monoclonal antibodies (isotype IgM) against E. corrodens LPS reacted with lipid A preparations from E. corrodens, Escherichia coli and Salmonella minesota.
It was found that the mitogenic activity and polyclonal B-cell activation of LPSs from E. corrodens and F. nucleatum were comparable to those of E. coli LPS. And these activities were partially inhibited by the monoclonal antibodies against LPS.
We produced antiーidiotype antibodies to antibody to lipid A from E. corrodens. The sera of mice and rabbits immunized with the antiーidiotype antibodies revealed that the antiーidiotype antibody induced the production of IgG antibodies which crossー reacted with lipid A. Immunization of mice with the antiーidiotype antibody resulted in prevention of lethal toxicity from LPS.
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