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Isolation of Phospholipase A_2 Activating Peptide From Inflamed Gingival Tissues.

Research Project

Project/Area Number 01571014
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionOsaka University, Faculty of Dentistry

Principal Investigator

SAITO Kihachi  Osaka University, Faculty of Dentistry, Pharmacology, Associate Professor, 歯学部, 助教授 (40110788)

Co-Investigator(Kenkyū-buntansha) MAEDA Sadaaki  Osaka University, Faculty of Dentistry, Pharmacology Research Assistant, 歯学部, 助手 (00135732)
INOKI Reizo  Osaka University, Faculty of Dentistry, Pharmacology Professor, 歯学部, 教授 (60028719)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPhospholipase A_2 / Peptide / Gingival tissues / Carrageenin induced granuloma / ホスホリパ-ゼA_2 / 実験的炎症 / 起炎 / 血管透過性
Research Abstract

It is well known that prostaglandins are intimately involved in inflammatory responses. Prostaglandins are synthesized by phospholipase A_2 (PLase A_2), a rate-limiting enzyme for their synthesis. Thus, group of drugs inhibiting PLase A_2 activity possess an anti-inflammatory action. On the contrary, we have reported the existence of PLase A_2 activation components in the extract of granuloma induced in the rat by carrageenin. The present study was initiated aiming for the demonstration of possible existence of this component in inflammatory gingival tissues.
1) Component activating PLase A_2 activity was isolated from an extract of granuloma induced in the rat by carrageenin. The isolation procedures consisted of extraction in 2 % phenol, adsorption on and desorption from charcoal, gel filtration on Sephadex G-25, G-10 and DEAE cellulose column. The material was finally isolated to homogeneity in high pressure liquid chromatography.
2) Approximate molecular weight estimation in Sephadex G-10 column gave 800. Digestion of component with leucine aminopeptidase resulted in the loss of PLase A_2 activation. These results indicated that PLase A_2 activating component is a peptide having small molecular weight.
3) Amino acid analysis of the PLase A_2 activating component gave a composition of amino acid of alanine, aspartic acid, leucine, phenylalanine and serine.
4) Trials of isolating the material from inflamed gingival tissues was unsuccessful. Another trial estimating the component in inflamed gingival tissues will be made following the production of antibody against the peptide prepared from carrageenin induced granuloma.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Wei E.ーQ: "Interaction between bradykinin and enkephalins in rat dental pulp" Acta Pharmacologica Sinica. 10. 500-503 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kudo T.: "Involvement of lysosomal enzymes in enkephalin production in dental pulp of the rat" Dentistry in Japan. 26. 41-44 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inoki R.: "A possible modulation of sensory transmission by opioid peptides at primary afferent neurons in trigeminal ganglion" Advances in Biosciences. 75. 427-430 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Inoki R.: "Influence of morphine on the descending monoaminergic systems to the trigeminal nucleus caudalis of the rabbit" Advances in Biosciences. 75. 423-426 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yonehara N.: "Involvement of descending monoaminergic system in the transmission of dental pain in the trigeminal nucleus caudalis of the rabbit" Brain Res.508. 234-240 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kudo T.: "KININSーV" Plenum, 5 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kudo T.: "The International Narcotics Research Conference(INRC)" Liss, 4 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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