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Effects of extracellular ATP and nerve growth factor on pheochromocytoma PC12 cells.

Research Project

Project/Area Number 01571027
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionMeikai University School of Dentistry

Principal Investigator

YAMADA Shozo  Meikai Univ. Sch. Dent. Biochemistry, Professor, 医学部, 教授 (30049358)

Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsExtracellular ATP / Pheochromocytoma PC12 / Nerve growth factor / Intracellular calcium ion / Cyclic AMP / P_2 purinergic receptor / Catecholamine release / Dopamine production / カルシウムチャンネル / 神経細胞分化 / 神経突起形成
Research Abstract

Effects of extracellular ATP on pheochrowxytoma PC12 and PC12h cells, and on the responses of the cells to nerve growth factor (NGF) were studied. 1) ATP inhibited the PC12h cell growth regardless with the presence or absence of NGF in the culture medium. 2) ATP enhanced the NGF-induced neurite outgrowth from PC12h cells : it increased the neurite length rather than the frequency of the neurite formation. ADP, AMP and adenosine (ADO) also showed the similar effect. Rank order of their potency was ATP>ADP>AMP=ADO. 3) ATP stimulated phosphorylation of a nuclear protein, SMP, of PC12 cells. SMP is a protein of which phosphorylation has been known to be enhanced by NGF. At lower concentration this enhancing effect was specific for ATP. At higher concentration (1 mM) AMP and ADO also showed the similar effect. 4) ATPinduced increase in cytosolic Ca^<2+> concentration of Pcl2h cells. Even in the absence of extracellular Ca^<2+>, about 40% of the maximal[Ca^<2+>]i increase was induced by ATP. ADP had the similar effect but AMP and ADO did not. 5) ATP induced a release of dopamine from PC12h cells. In the absence of extracellular Ca^<2+>, less than 10% of dopamine release detected in the presence of Ca^<2+> was observed, indicating that Ca^<2+> introduced into the cells by influx may play the major role in ATP-evoked catecholamine release. In the short term responses of the cells to ATP, adenine nucleotide specificity for ATP was quite clear. On the other hand, ATP is known to metabolized to ADP, AMP and ADO in sermcontaining culture medium and in body fluid. Therefore, the responses of the cells observed by long-term treatment with ATP may be resulted from a complex effect of ATP mediated by P_2 receptors and that of AMP and ADO mediated by Adenosine receptors.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] 中川 幹也: "Teratoma 402X細胞のNADPH特異性ジヒドロプテリジン還元酵素に対するretinoic acid,dibutyyl cyclic AMP,および神経成長因子の影響." 明海歯学誌. 19. 94-101 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Simultaneous determination of GTP cyclohydrolase activity and biopterin content in pheochromocytoma PC12h cells." J.Meikai Univ.Sch.Dent.19. 197-203 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kaoruko AONO: "Increase in intracellular Ca^<2+> level and modulation of nerve growth factor action on pheochromocytoma PC12h cells by extracellular ATP." J.Meikai Univ.Sch.Dent.19. 221-229 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小野沢 智史: "In vivoおよびin vitroにおけるpheochromocytoma PC12h細胞のdopmaine生成能." 明海歯学誌. 20. 151-157 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小野沢 智史: "褐色細胞腫細胞PC12hにるノルエピネフリン取り込みと細胞内ド-パ,ド-パミン含量に対するreserpineおよびnomifensineの影響." 明海歯学誌. 22.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Effect of nerve growth factor and dibutyryl cyclic AMP on biopterin and catecholamine levels of PC12h pheochromocytoma cells." Pteridines. 3. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "ATP-induced release of catecholamines from pheochromocytoma PC12h cells."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Cyclic AMP inhibition of catecholamine transport by pheochromocytoma PC12h cells."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Enzymes in glycolytic pathway.In:Dynamic Aspect of Dental Pulp (Inoki,R.,Kudo,T.,& Olgart,L.,eds.)" Chapman and Hall,New York, 18 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Effect of actinomycin D and cycloheximide on the nerve growth factor (NGF)-induced increase in biopterin level of pheochromocytoma PC12h cells.in Pterins and biogenic Amines in Neurolog,Pediatrics,and Immunology.(Blau,N.,Curtius,H.C.,Levine,R.A.,& Cotton,R.G.H.,eds.)" Lakeshore Publishing,Grosse Pointe,USA, 8 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "GTP cyclohydrolase of pheochromocytoma PC12h cells:Effect of nerve growth factor (NGF) treatment of the cells.In:Pterins and Biogenic Amines in Neurology,Pediatrics,and Immunology.(Blau,N.,Curtius,H.C.,Levine,R.A.,&Cotton,R.G.H.,eds.)" Lakeshore Publishing,Grosse Pointe,USA, 9 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mikiya NAKAGAWA: "Effect of retinoic acid, dibutyryl cyclic AMP, and nerve growth factor on NADPH-specific dihydropteridine reductase of teratoma 402AX cells in cyulture." J. Meikai Univ. Sch. Dent.19. 94-101 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Simultaneous determination of GTP cyclohydrolase activity and biopterin content in pheochromocytoma PC12h cells." J. Meikai Univ. Sch. Dent.19. 197-203 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kaoruko AONO: "Increase in intracellular Ca^<2+> level and modulation of nerve growth factor action on pheochromocytoma PC12h cells by extracellular ATP." J. Meikai Univ. Sch. Dent.19. 221-229 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Enzymes in glycolytic pathway." Dynamic Aspect of Dental Pulp. Chapman and Hall, New York. 203-220 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoshi ONOZAWA: "In vivo and in vitro assays for the dopamine-producing activity of rat pheochromocytoma PC12h cells." J. Meikai Univ. Sch. Dent.20. 151-157 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Effect of actinomycin D and cycloheximide on the nerve growth factor (NGF)-induced increase in biopterin level of pheochromocytoma PC12h cells." Pterins and Biogenic Amines in Neurolog, Pediatrics, and in Neurolog, Pediatrics, and Immunology.Lakeshore Publishing, Grosse Ponite. 101-108 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "GTP cyclohydrolase of pheochromocytoma PC12h cells : Effect of nerve growth factor (NGF) treatment of the cells." Pterins and Biogenic Amines in Neurolog, Pediatrics, and in Neurolog, Pediatrics, and Immunology.Lakeshore Publishing, Grosse Ponite. 108-117 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Satoshi ONOZAWA: "Inhibition of norepinephrine uptake of pheochromo-cytoma cells by reserpine and nomifensine and their effects on dopa and dopamine contents of the cells." J. Meikai Univ. Sch. Dent. 21.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Effect of nerve growth factor and dibutyryl cyclic AMP on biopterin and catecholamine levels of PC12h pheochromocytoma cells." Pteridines. 3.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "ATP-Induced release of catecholamines from pheochromocytoma PC12h cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nobuo NAKANISHI: "Cyclic ATP inhibition of catecholamine transport by pheochromocytoma PC12h cells."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小野沢 智史: "In vivoおよびin vitroにおけるpheochromocytoma PC12h細胞のdopmaine生成能。" 明海歯学誌. 20,. 151-157 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小野沢 智史: "褐色細胞腫細胞PC12hにるノルエピネフリン取り込みと細胞内ド-パ,ド-パミン含量に対するreserpineおよびnomifensineの影響。" 明海歯学誌. 21. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Effect of nerve growth factor and dib utyryi cyclic AMP on biopterin and catecholamine levels of PC12h Pheochromoc" Pteridines. 1992.

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "ATPーinduced release of catecholamines from pheochromocytoma PC12h cells."

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Cyclic AMP inhibition of catecholamine transport by pheochromocytoma PC12h cells."

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Effect of actinomycin D and cycloheximide on the nerve growth factor(NGF)ーinduced increase in biopterin level of pheochromocytoma PC12h cells.in Pterins and Biogenic Amines in Neurolog,Pediatrics,and Immunology.(Blau,N.,Curtius,H.C.,Levine,R.A.,& Cotton,R.G.H.,eds.)" Lakeshore Publishing,Grosse Pinte,USA, 8 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "GTP cyclohydrolase of pheochromocytoma PC12h cells.in Pterins and Biogenic Amines in Neurolog,Pediatrics,and Immunology.(Blau,N.,Curtius,H.C.,Levine,R.A.,& Cotton,R.G.H.,eds.)" Lakeshore Publishing,Groose Pointe,USA, 9 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Simultaneous determination of GTP cyclohydrolase activity and biopterin content in pheochromocytoma PC12h cells." J.Meikai Univ.Sch.Dent.19. 197-203 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kaoruko AONO: "Increase in intracellular Ca^<2+> level and modulation of nerve growth factor action on pheochromocytoma PC12h cells by extracellular ATP." J.Meikai Univ.Sch.Dent.19. 221-229 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Effect of actinomycin D and cycloheximide on the nerve growth factorーinduced increase in biopterin level of pheochromocytoma PC12h cells." Pteridines and Related Biogenic Amines in Neuropsychiatry, Pediatrics Pediatrics, and Immunology 1990.(Blau,N.,Curtius,H.Ch.,Levine,R.A.&Cotton,G.H.R.,eds.)Lakeshore Publishing,Grosse Pointe.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "GTP cyclohydrolase of pheochromocytoma PC12h cells: Effect of nerve growth factor treatment of the cells." Pteridines and Related Biogenic Amines in Neuropsychiatry,Pediatrics Pediatrics,and Immunology 1990.(Blau,N.,Curtius,H.Ch.,Levine,R.A.&Cotton,G.H.R.,eds.)Lakeshore Publishing, Grosse Pointe.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "Effect of actinomycin D and cycloheximide on the nerve growth factor(NGF)-induced increase in BP level of pheochromocytoma PC12h cells." Pteridiens. 2. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "GTP cyclohydrolase of pheochromocytoma PC12h cells:Effect of nerve growth factor(NGF)treatment of the cells." Pteridiens. 2. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 中川幹也: "Teratoma 402AX 細胞のNADH特異性ジヒドロプテリジン還元酵素に対するretinoic acid,dibutyryl cyclic AMP、および神経成長因子の影響" 明海大学歯学雑誌. 19. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kaoruko AONO: "Extracellular ATP increases cytosolic calcium ion level and enhances nerve growth factor-induced neurite outgrowth from PC12h pheohromocytoma cells."

    • Related Report
      1989 Annual Research Report
  • [Publications] Nobuo NAKANISHI: "“Enzymes in glycolytic pathway"in Dynamic Aspects of Dental Pulp(Inoki,R.,Kudo,T.,& Olgart,L.,eds.)pp.203-220" Chapman and Hall ltd., (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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