| Project/Area Number |
01571036
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
MORI Masakazu Osaka Dental University, Pharmacology, Professor, 歯学部, 教授 (20066963)
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| Co-Investigator(Kenkyū-buntansha) |
OGATA Keigo Osaka Dental University, Pharmacology, Assistant, 歯学部, 助手 (30214000)
SHINOHARA Mitsuko Osaka Dental University, Pharmacology, Lecturer, 歯学部, 講師 (40067187)
OHURA Kiyoshi Osaka Dental University, Pharmacology, Associate Professor, 歯学部, 助教授 (20131378)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Local anesthetic / Rat / Human / Leukocytes / Active oxygens / Chemotaxis / Phagocytosis / 貧食能 |
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| Research Abstract |
The effects of 5 local anesthetics (lidocaine, prilocaine, mepivacaine, procaine and tetracaine) on the production of oxygen radicals (O_2 - and H_2O_2) together with chemotaxis and phagocytosis of both human and rat leukocytes (polymorphonuclear leukocytes and macrophages) were examined. Production of oxygen radicals was measured in microtiter plates using opsonized zymosan (OPZ), phorbol myristate acetate or calcium ionophore A23187 as a stimulant. Chemotaxis was measured by membrane filter method using serum or FMLP as a chemoattractant. Phagocytosis was measured by light microscopic count of OPZ engulfing cells. Each local anesthetic dose-dependently inhibited O_2 - and H_2O_2 production of both human and rat leukocytes and the inhibition was reversible. Stimulants showed different oxygen radical production ability among tree. Local anesthetics also dose-dependently inhibited both chemotaxis and phagocytosis. These results suggest that correlation between overuse of a local anesthetic in dental therapy such as an extraction of a tooth and postoperative infection.
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