| Project/Area Number |
01571101
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Showa university |
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Principal Investigator |
SAKURADA Shigeyo Showa University, Dental School, Assistant Prof., 歯学部, 講師 (00112730)
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| Co-Investigator(Kenkyū-buntansha) |
IWASE Masayasu Showa University, Dental School, Assistant Prof., 歯学部, 講師 (50193743)
西村 明之 昭和大学, 歯学部, 助手 (50150652)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | cancer cell line / anticancer drugs / superoxide dismutase / catalase / superoxide anion / bleomycin / superoxide dismutase / 口腔悪性腫瘍 / 口腔癌由来株細胞 |
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| Research Abstract |
The effect of anticancer drugs on superoxide dismutase (SOD) was investigated. Two cancer cell lines were used in this experiment, they are HeLa S3 cells and NA cells which originated from a human squamous cell carcinoma of the tongue, were established as a cell line in our laboratory in 1981. Bleomycin, mitomycin C, cisplatin, and 5-fluorouracil were investigated as anticancer drugs. SOD activity in the cancer cells were found to be enhanced after administration of these drugs ; SOD activity increased in a dose-dependent menner at concentrations from 1.2 to 30 mug/ml of bleomycin, and reached maximam 6 hores after the administration of 2.0 mug/ml of the drug. NaF, a glycolysis inhibitor, enhanced SOD activity both in the presence and absence of bleomycin, while KCN decreased SOD activity significantly. NaF and bleomycin showed and additive effect. These results suggest that SOD activitymay be enhanced by anticancer drugs through several mechanism.
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