| Project/Area Number |
01571102
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Showa University |
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Principal Investigator |
KANEMOTO Keiko (1990) Showa University, Dental School, Assistant, 歯学部, 助手 (30177547)
吉屋 誠 (1989) 昭和大学, 歯学部, 講師 (00119281)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Atushi Showa University, Dental School, Assistant, 歯学部, 助手 (30192090)
YOSHIYA Makoto Showa University, Dental School, Assistant Prof., 歯学部, 兼任講師
金本 恵子 昭和大学, 歯学部, 助手 (30177547)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Squamous cell carcinoma / Fibronectin / Metastasis of cancer / Cancer cell line / Differentiation of tumor / Immunoperoxidase technique / ELISA |
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| Research Abstract |
Regarded to play some roles in metastasis of squamous cell carcinoma (SCC), fibronectin might be a useful marker for grade of tongue SCC. A fibronectin-producing cell line, called NA cell, has been established from explant culture of a primary squamous cell carcinoma (SCC) of tongue.
The location of fibronectin was investigated with an abidin-biotin complex immunoperoxidase technique (ABC), revealing fibronectin distributed on the cell surface and in the cytoplasm around the nuclei. The synthesis of fibronectin was then examined by an enzyme-linked immunosorbent assay (ELISA), revealing an increase of fibronectin in the conditioned media in a time-dependent manner until the cell reached the stationary phase.
As these data indicate, NA cells may be useful for studying mechanisms of metastasis and invasion of tongue cancer.
The distribution of fibronectin in patients who had tongue SCC was investigated with an ABC technique. Five specimen from tongue, and two specimen from metastatic lesional lymph nods were examined. Two cases of well-differentiated SCC showed fibronectin in tumor cells and stroma. Three cases of undifferentiated SCC revealed fibronectin in stroma, but little distribution was seen in tumor cells. And one of these three cases which only stroma showed positive staining for fibronectin proceeded poor progress. Two cases of metastatic lymph nods revealed very little positive staining for fibronectin both in stroma and basal layer of tumor cells also had poor progress.
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